Biological Trace Element Research

, Volume 154, Issue 1, pp 28–44

Selenium Supplementation Alters Gene Expression Profiles Associated with Innate Immunity in Whole-Blood Neutrophils of Sheep

  • Hugejiletu Hugejiletu
  • Gerd Bobe
  • William R. Vorachek
  • M. Elena Gorman
  • Wayne D. Mosher
  • Gene J. Pirelli
  • Jean A. Hall
Article

DOI: 10.1007/s12011-013-9716-6

Cite this article as:
Hugejiletu, H., Bobe, G., Vorachek, W.R. et al. Biol Trace Elem Res (2013) 154: 28. doi:10.1007/s12011-013-9716-6

Abstract

Footrot (FR) is a common, contagious bacterial disease of sheep that results in lameness and significant economic losses for producers. We previously reported that sheep affected with FR have lower whole-blood (WB) selenium (Se) concentrations and that Se supplementation in conjunction with routine control practices accelerates recovery from FR. To determine whether oral Se-yeast administered at supranutritional levels (>4.9 mg Se/week) alters the ability of sheep to resist or recover from FR infection, 60 ewes with and 60 ewes without FR were drenched once weekly for 62.5 weeks with 0, 4.9, 14.7, or 24.5 mg organic Se-yeast (30 ewes per treatment group). Footrot prevalence and severity were measured at 0, 20, 28, 40, and 60 weeks of Se supplementation. Genomic expression of eight WB-neutrophil genes for selenoproteins and seven WB-neutrophil genes for proteins involved in innate immunity was determined at the end of the treatment period using SYBR Green and quantitative polymerase chain reaction methodology. Supranutritional Se-yeast supplementation successfully increased Se status in sheep but did not prevent FR. Supranutritional Se-yeast supplementation increased WB-neutrophil expression of genes involved in innate immunity: l-selectin, interleukin-8 receptor, and toll-like receptor 4, which were or tended to be lower in ewes affected with FR. Furthermore, supranutritional Se-yeast supplementation altered the expression of selenoprotein genes involved in innate immunity, increasing selenoprotein S and glutathione peroxidase 4 and decreasing iodothyronine deiodinases 2 and 3. In conclusion, supranutritional Se-yeast supplementation does not prevent FR, but does alter WB-neutrophil gene expression profiles associated with innate immunity, including reversing those impacted by FR.

Keywords

Blood neutrophils Footrot Gene expression Immune function genes Selenium supplementation Selenoprotein genes Sheep 

Abbreviations

COX II

Cyclooxygenase II

Dio

Iodothyronine deiodinases

FBS

Fetal bovine serum

FOS

FBJ murine osteosarcoma viral oncogene homolog or c-FOS, and a member of the activator protein-1 family

FR

Footrot

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GPx

Glutathione peroxidases

HBSS

Hank's balanced saline solution

ICP-MS

Ionized coupled plasma mass spectrometry

IFN-γ

Interferon-gamma

IL-8R

Interleukin-8 receptor

l-Sel

l-Selectin

NFκB

Nuclear factor kappa B

PBS

Phosphate buffered saline

PPM1A

Protein phosphatase magnesium-dependent 1A

qPCR

Quantitative polymerase chain reaction

RBC

Red blood cells

ROS

Reactive oxygen species

RPL19

Ribosomal protein large subunit family member-19

Se

Selenium

SeCys

Selenocysteine

SeMet

Selenomethionine

SEP

Selenoproteins

TBK1

TANK-binding kinase 1

TLR4

Toll-like receptor 4

TxnR

Thioredoxin reductase

WB

Whole blood

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Hugejiletu Hugejiletu
    • 1
    • 2
  • Gerd Bobe
    • 3
    • 4
  • William R. Vorachek
    • 1
  • M. Elena Gorman
    • 1
  • Wayne D. Mosher
    • 3
  • Gene J. Pirelli
    • 3
  • Jean A. Hall
    • 1
  1. 1.Department of Biomedical Sciences, College of Veterinary MedicineOregon State UniversityCorvallisUSA
  2. 2.College of Veterinary MedicineInner Mongolian Agricultural UniversityHohhotChina
  3. 3.Department of Animal and Rangeland Sciences, College of Agricultural SciencesOregon State UniversityCorvallisUSA
  4. 4.Linus Pauling InstituteOregon State UniversityCorvallisUSA

Personalised recommendations