Biological Trace Element Research

, Volume 154, Issue 2, pp 299–303 | Cite as

Influence of Selenium on Mast Cell Mediator Release

  • Reza Safaralizadeh
  • Maryam Nourizadeh
  • Ahad Zare
  • Gholam Ali Kardar
  • Zahra PourpakEmail author


Selenium supplementation still enhanced the immune response even in individuals who, according to current standards, would be considered as not being overtly selenium deficient. Mast cells are granulated cells that play a pivotal role in allergic reactions. In this study, we investigated the modulatory effect of sodium selenite on mediator release and degranulation of murine mast cell line (MC/9). Cells were pre-treated with selenium selenite (1, 2, 3 μg/ml) for 24 h and controls left untreated. Then, cells were sensitized overnight with anti-dinitrophenyl (DNP) IgE and challenged with DNP/HSA for degranulation induction. The histamine and prostaglandin D2 (PGD2) were measured by ELISA, and β-hexosaminidase was measured by spectrophotometery method. Selenium-treated cells revealed significant decrease in concentration of PGD2 (P = 0.019) and β-hexosaminidase (P = 0.009). In addition, a slight reduction of histamine release by the selenium-treated cells was observed, based on our intracellular and extracellular assessments. The most inhibitory effect of selenium supplementation on mediator release of MC/9 cells was obtained in the presence of 3 μg/ml of sodium selenite. The results of the present study demonstrate beneficial effects of supplemental selenium in attenuating clinical manifestations of allergy and asthma.


Murine mast cell line Selenium Histamine Prostaglandin D2 β-Hexosaminidase 



This research has been supported by a grant from the Tehran University of Medical Sciences (grant code 88-04-40-10238).


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Reza Safaralizadeh
    • 1
  • Maryam Nourizadeh
    • 2
  • Ahad Zare
    • 2
  • Gholam Ali Kardar
    • 2
  • Zahra Pourpak
    • 2
    • 3
    Email author
  1. 1.Department of Animal Biology, Faculty of Natural ScienceUniversity of TabrizTabrizIran
  2. 2.Immunology Asthma and Allergy Research InstituteTehran University of Medical SciencesTehranIran
  3. 3.Departmen of Immunology and Allergy, Children Medical CenterTehran University of Medical SciencesTehranIran

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