Biological Trace Element Research

, Volume 152, Issue 3, pp 297–299 | Cite as

Evaluating the Suitability of Postnuclear Supernatants as In Vitro Models for Assessing Cadmium- and Other Xenobiotic-Induced Neurotoxicity on Crucial Enzymatic Parameters

  • Apostolos Zarros
  • Ryan T. Cameron
  • Stylianos Tsakiris
  • George S. Baillie

Govil et al. [1] have recently provided an interesting report on the use of murine brain-derived postnuclear supernatants as an in vitro model for the assessment of neurotoxic effects of cadmium (Cd). The authors focus on an important topic within the neurotoxicity arena and describe an extensive neurochemical screening via the study of the in vitro effects of different Cd-chloride (CdCl2) concentrations (1, 5, and 10 mM) on the following crucial enzymatic and nonenzymatic parameters: catalase activity, superoxide dismutase activity, glutathione-S-transferase activity, acetylcholinesterase (AChE) activity, sodium–potassium adenosinetriphosphatase (Na+, K+-ATPase) activity, reduced glutathione content, and thiol(s) content analysis [1]. It is our opinion that the methodology used by the authors for the neurochemical analysis is appropriate and so is the studied biomarker selection. However, there are certain important issues addressed within this report [1], on which we would like to...


CdCl2 Adenosinetriphosphatase CdSO4 Concentration Window Toxic Encephalopathy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Conflict of interest

All authors (AZ, RTC, ST, and GSB) declare that there are no conflicts of interest.


  1. 1.
    Govil N, Chaudhary S, Waseem M, Parvez S (2012) Postnuclear supernatant: an in vitro model for assessing cadmium-induced neurotoxicity. Biol Trace Elem Res 146:402–409PubMedCrossRefGoogle Scholar
  2. 2.
    Carageorgiou H, Tzotzes V, Pantos C, Mourouzis C, Zarros A, Tsakiris S (2004) In vivo and in vitro effects of cadmium on adult rat brain total antioxidant status, acetylcholinesterase, (Na+, K+)-ATPase and Mg2+-ATPase activities: protection by L-cysteine. Basic Clin Pharmacol Toxicol 94:112–118PubMedCrossRefGoogle Scholar
  3. 3.
    Schulpis KH, Kalimeris K, Bakogiannis C, Tsakiris T, Tsakiris S (2006) The effect of in vitro homocystinuria on the suckling rat hippocampal acetylcholinesterase. Metab Brain Dis 21:21–28PubMedCrossRefGoogle Scholar
  4. 4.
    Simintzi I, Schulpis KH, Angelogianni P, Liapi C, Tsakiris S (2007) The effect of aspartame metabolites on the suckling rat frontal cortex acetylcholinesterase. An in vitro study. Food Chem Toxicol 45:2397–2401PubMedCrossRefGoogle Scholar
  5. 5.
    Zarros A, Theocharis S, Skandali N, Tsakiris S (2008) Effects of fulminant hepatic encephalopathy on the adult rat brain antioxidant status and the activities of acetylcholinesterase, (Na+, K+)-and Mg2+-ATPase: comparison of the enzymes’ response to in vitro treatment with ammonia. Metab Brain Dis 23:255–264PubMedCrossRefGoogle Scholar
  6. 6.
    Sgaravatti AM, Vargas BA, Zandoná BR, Deckmann KB, Rockenbach FJ, Moraes TB, Monserrat JM, Sgarbi MB, Pederzolli CD, Wyse AT, Wannmacher CM, Wajner M, Dutra-Filho CS (2008) Tyrosine promotes oxidative stress in cerebral cortex of young rats. Int J Dev Neurosci 26:551–559PubMedCrossRefGoogle Scholar
  7. 7.
    Moraes TB, Zanin F, da Rosa A, de Oliveira A, Coelho J, Petrillo F, Wajner M, Dutra-Filho CS (2010) Lipoic acid prevents oxidative stress in vitro and in vivo by an acute hyperphenylalaninemia chemically-induced in rat brain. J Neurol Sci 292:89–95PubMedCrossRefGoogle Scholar
  8. 8.
    Fernandes CG, Leipnitz G, Seminotti B, Amaral AU, Zanatta A, Vargas CR, Dutra Filho CS, Wajner M (2010) Experimental evidence that phenylalanine provokes oxidative stress in hippocampus and cerebral cortex of developing rats. Cell Mol Neurobiol 30:317–326PubMedCrossRefGoogle Scholar
  9. 9.
    Skrzycki M, Czeczot H, Majewska M, Podsiad M, Karlik W, Grono D, Wiechetek M (2010) Enzymatic antioxidant defense in isolated rat hepatocytes exposed to cadmium. Pol J Vet Sci 13:673–679PubMedGoogle Scholar
  10. 10.
    Xie HR, Hu LS, Li GY (2010) SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson’s disease. Chin Med J (Engl) 123:1086–1092Google Scholar
  11. 11.
    Chan KH, Lam KS, Cheng OY, Kwan JS, Ho PW, Cheng KK, Chung SK, Ho JW, Guo VY, Xu A (2012) Adiponectin is protective against oxidative stress induced cytotoxicity in amyloid-beta neurotoxicity. PLoS One 7:e52354PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Apostolos Zarros
    • 1
    • 2
  • Ryan T. Cameron
    • 1
  • Stylianos Tsakiris
    • 2
  • George S. Baillie
    • 1
  1. 1.Gardiner Laboratory (535), Wolfson Link Building, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life SciencesUniversity of GlasgowGlasgowUK
  2. 2.Laboratory of Physiology, Medical SchoolNational and Kapodistrian University of AthensAthensHellenic Republic

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