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Biological Trace Element Research

, Volume 152, Issue 1, pp 82–90 | Cite as

Influence of Rare Earth Elements on Metabolism and Related Enzyme Activity and Isozyme Expression in Tetrastigma hemsleyanum Cell Suspension Cultures

  • Peng Xin
  • Zhou Shuang-Lin
  • He Jun-Yao
  • Ding Li
Article

Abstract

The effects of rare earth elements (REEs) not only on cell growth and flavonoid accumulation of Tetrastigma hemsleyanum suspension cells but also on the isoenzyme patterns and activities of related enzymes were studied in this paper. There were no significant differences in enhancement of flavonoid accumulation in T. hemsleyanum suspension cells among La3+, Ce3+, and Nd3+. Whereas their inductive effects on cell proliferation varied greatly. The most significant effects were achieved with 100 μM Ce3+and Nd3+. Under treatment over a 25-day culture period, the maximal biomass levels reached 1.92- and 1.74-fold and the total flavonoid contents are 1.45- and 1.49-fold, than that of control, respectively. Catalase, phenylalanine ammonia-lyase (PAL), and peroxidase (POD) activity was activated significantly when the REE concentration range from 0 to 300 μM, whereas no significant changes were found in superoxide dismutase activity. Differences of esterase isozymes under REE treatment only laid in expression level, and there were no specific bands. The expression level of some POD isozymes strengthened with increasing concentration of REEs within the range of 50–200 μM. When REE concentration was higher than 300 μM, the expression of some POD isozymes was inhibited; meanwhile, some other new POD isozymes were induced. Our results also showed REEs did not directly influence PAL activity. So, we speculated that 50–200 μM REEs could activate some of antioxidant enzymes, adjust some isozymes expression, trigger the defense responses of T. hemsleyanum suspension cells, and stimulate flavonoid accumulation by inducing PAL activity.

Keywords

Tetrastigma hemsleyanum Suspension cells Rare earth Metabolism Isozyme 

Abbreviations

REEs

Rare earth elements

PAL

Phenylalanine ammonia-lyase

SOD

Superoxide dismutase

NAA

Naphthalene acetic acid

EST

Esterase

FW

Fresh weight

CAT

Catalase

ROS

Reactive oxygen species

POD

Peroxidase

6-BA

6-Benzyladenine

DW

Dry weight

Notes

Acknowledgments

This work was supported by the Natural Science Foundation of Ningbo City (grant no. 2012A610179) and the New Shoot Talents Program of Zhejiang Province (grant no. 2010R433006).

References

  1. 1.
    Xu CJ, Ding GQ, Fu JY et al (2008) Immunoregulatory effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et. Gilg on immune functions of ICR mice. Biomed Environ Sci 21(4):325–331PubMedCrossRefGoogle Scholar
  2. 2.
    He FG (2010) Research progress in anticancer effect of Tetrastigma hemsleyanum Diels et Gilg and its mechanism. J Oncol 16:75–77 (in Chinese)Google Scholar
  3. 3.
    Peng X, Zhang J, He JY (2012) Comparison on accumulation of flavonoids in loose and compact callus suspension cell culture of Tetrastigma hemsleyanum. Chin Tradit Herb Drugs 43:577–580 (in chinese)Google Scholar
  4. 4.
    Karwasara VS, Jain R, Tomar P et al (2010) Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn. In Vitro Cell Dev Biol Plant 46:354–362CrossRefGoogle Scholar
  5. 5.
    Chakraborty A, Chattopadhyay S (2008) Stimulation of menthol production in Mentha piperita cell culture. In Vitro Cell Dev Biol Plant 44:518–524CrossRefGoogle Scholar
  6. 6.
    Karwasara VS, Dixit VK (2012) Culture medium optimization for improved puerarin production by cell suspension cultures of Pueraria tuberosa (Roxb. ex Willd.) DC. In Vitro Cell Dev Biol Plant 48:189–199CrossRefGoogle Scholar
  7. 7.
    Ge F, Wang XD, Zhao B, Wang YC (2006) Effects of rare earth elements on the growth of Arnebia euchroma cells and the biosynthesis of shikonin. Plant Growth Regul 48:283–290CrossRefGoogle Scholar
  8. 8.
    Yuan XF, Zhao B, Wang YC (2005) Application of rare earth elements in medicinal plant cell and tissue culture. Chin Bull Bot 22:115–120Google Scholar
  9. 9.
    Zhou M, Gong X, Wang Y et al (2011) Improvement of cerium of photosynthesis functions of maize under magnesium deficiency. Biol Trace Elem Res 142:760–772PubMedCrossRefGoogle Scholar
  10. 10.
    Liu XQ, Ze YG, Liu C et al (2009) Effects of Ce3+ on improvement of spectral characteristics and function of chloroplasts damaged by linolenic acid in spinach. J Rare Earths 27:288–293CrossRefGoogle Scholar
  11. 11.
    Huang H, Chen L, Liu XQ et al (2008) Absorption and transfer of light and photoreduction activities of spinach chloroplasts under calcium deficiency: promotion by cerium. Biol Trace Elem Res 122:157–167CrossRefGoogle Scholar
  12. 12.
    Huang G, Wang L, Zhou Q (2012) Lanthanum (III) regulates the nitrogen assimilation in soybean seedlings under ultraviolet-B radiation. Biol Trace Elem Res. doi: 10.1007/s12011-012-9528-0
  13. 13.
    Olivares E, Aguiar G, Colonnello G (2011) Rare earth elements in vascular plants: a review. Interciencia 36(5):331–340Google Scholar
  14. 14.
    Diatloff E, Smith FW, Asher CJ (2008) Effects of lanthanum and cerium on the growth and mineral nutrition of corn and mungbean. Ann Bot 101(7):971–982PubMedCrossRefGoogle Scholar
  15. 15.
    d’Aquino L, Massimo M, Carboni MA (2009) Effect of some rare earth elements on the growth and lanthanide accumulation in different Trichoderma strains. Soil Biol Biochem 41:2406–2413CrossRefGoogle Scholar
  16. 16.
    Boyko A, Matsuoka A, Kovalchuk I (2011) Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation. Plant Cell Rep 30:505–518PubMedCrossRefGoogle Scholar
  17. 17.
    Wang CT, Shi GX, Xu QS (2005) Toxic effect of Cd2+ on Potamogeton crispus alleviated by exogenous Nd3+. J Rare Earths 23(6):752–755 (in chinese)Google Scholar
  18. 18.
    Liu YJ, Wang Y, Wang FB (2008) Control effect of lanthanum against plant disease. J Rare Earths 26:115–120CrossRefGoogle Scholar
  19. 19.
    Osorio S, Alba R, Damasceno CM et al (2011) Systems biology of tomato fruit development: combined transcript, protein, and metabolite analysis of tomato transcription factor and ethylene receptor mutants reveals novel regulatory interactions. Plant Physiol 157(1):405–425PubMedCrossRefGoogle Scholar
  20. 20.
    Bowler C, Camp WV, Montagy MV et al (1994) Superoxide dismutase in plants. Crit Rev Plant Sci 13(3):199–218Google Scholar
  21. 21.
    Ippolito MP, Fasciano C, d’Aquino L et al (2010) Responses of antioxidant systems after exposition to rare earths and their role in chilling stress in common duckweed (Lemna minor L.): a defensive weapon or a boomerang? Arch Environ Contam Toxicol 58:42–52PubMedCrossRefGoogle Scholar
  22. 22.
    Fl Z, Tian HE, Wang ZP et al (2003) Effect of rare earth element europium on amaranthin synthesis in Amarathus caudatus seedlings. Biol Trace Elem Res 93:271–282CrossRefGoogle Scholar
  23. 23.
    Zhishen JT, Mengcheng T, Jianming W (1999) Research on antioxidant activity of flavonoids from natural materials. Food Chem 64:555–559CrossRefGoogle Scholar
  24. 24.
    Cipollini DF Jr (1998) The induction of soluble peroxidase activity in bean leaves by wind-induced mechanical perturbation. Am J Bot 85(11):1586–1591PubMedCrossRefGoogle Scholar
  25. 25.
    Wang YS, Tian SP, Xu Y et al (2004) Changes in the activities of pro- and anti-oxidant enzymes in peach fruit inoculated with Cryptococcus laurentii or Penicillium expansum at 0 or 20°C. Postharvest Biol Technol 34:21–28CrossRefGoogle Scholar
  26. 26.
    Peng X, Jin WT, Ling QZ (2012) Changes of phenolics content and related enzymatic activities in Fritillaria thunbergii Miq. and their relationships with dormancy release. Med Plant 3(6):44–47Google Scholar
  27. 27.
    Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assay applicable to acrylamide gels. Anal Biochem 44:276–287PubMedCrossRefGoogle Scholar
  28. 28.
    Mandak B, Bimova K, Pysek P (2005) Isoenzyme diversity in Reynoutria (Polygonaceae) taxa: escape from sterility by hybridization. Plant Syst Evol 253:219–230CrossRefGoogle Scholar
  29. 29.
    Huang WH (2011) Biological effect of rare earth element and genetic transformation on Arnebia euehroma (Royle) Johnst. cell. Dissertation, University of Kunming Science and Technology (in Chinese)Google Scholar
  30. 30.
    Ozaki T, Enomoto S, Minai Y (2000) Beneficial effect of rare earth elements on the growth of Dryopteris erythrosora. J Plant Physiol 156:330–334CrossRefGoogle Scholar
  31. 31.
    Gao YS, Zeng FL, Yi A (2003) Research of the entry of rare earth elements Eu3+ and La3+ into plant cell. Biol Trace Elem Res 91:253–265PubMedCrossRefGoogle Scholar
  32. 32.
    Wei ZG, Hong FS, Yin M et al (2005) Structural differences between light and heavy rare earth element binding chlorophylls in naturally grown fern Dicranopteris linearis. Biol Trace Elem Res 106:279–297PubMedCrossRefGoogle Scholar
  33. 33.
    Liang T, Yan BZ, Zhang S (2001) Contents and the biogeochemical characteristics of rare earth elements in wheat seeds. Biogeochemistry 54:41–49CrossRefGoogle Scholar
  34. 34.
    Ouyang J, Xiaodong W, Bing Z et al (2003) Effects of rare earth elements on the growth of Cistanch deserticola cells and the production of phenylethanoid glycosides. J Biotechnol 102:129–134PubMedCrossRefGoogle Scholar
  35. 35.
    Yuan XF, Wang Q, Zhao B et al (2002) Improved cell growth and total flavonoids of Saussurea medusa on solid culture medium supplemented with rare earth elements. Biotechnol Lett 24:1889–1892CrossRefGoogle Scholar
  36. 36.
    Jing F, Bei W, Shan XQ et al (2007) Evaluation of bioavailability of light rare earth elements to wheat (Triticum aestivum L.)under field conditions. Geoderma 141:53–59CrossRefGoogle Scholar
  37. 37.
    Wu JY, Wang CG, Mei XG (2001) Stimulation of taxol production and excretion in Taxus spp cell cultures by rare earth chemical lanthanum. J Biotechnol 85:67–73PubMedCrossRefGoogle Scholar
  38. 38.
    Zhou J, Fang L, Li X et al (2012) Jasmonic acid (JA) acts as a signal molecule in LaCl3-induced baicalin synthesis in Scutellaria baicalensis seedlings. Biol Trace Elem Res 148:392–395PubMedCrossRefGoogle Scholar
  39. 39.
    Liu C, Cao WQ, Lu Y et al (2009) Cerium under calcium deficiency influence on the antioxidative defense system in spinach plants. Plant and Soil 323:285–294CrossRefGoogle Scholar
  40. 40.
    Ze YG, Zhou M, Luo LY et al (2009) Effects of cerium on key enzymes of carbon assimilation of spinach under magnesium deficiency. Biol Trace Elem Res 131(2):154–164CrossRefGoogle Scholar
  41. 41.
    Yang GM, Sun ZG, Lv XF et al (2012) Living target of Ce(III) action on horseradish cells: proteins on/in cell membrane. Biol Trace Elem Res. doi: 10.1007/s12011-012-9514-6
  42. 42.
    Guo B, Xu L, Guan ZJ et al (2012) Effect of lanthanum on rooting of in vitro regenerated shoots of Saussurea involucrata Kar. et Kir. Biol Trace Elem Res 147:334–340PubMedCrossRefGoogle Scholar
  43. 43.
    Wang L, Huang X, Zhou Q (2009) Protective effect of rare earth against oxidative stress under ultraviolet-B radiation. Biol Trace Elem Res 128:82–93PubMedCrossRefGoogle Scholar
  44. 44.
    Paola M, Paciolla C, d'Aquino L et al (2007) Effect of rare earth elements on growth and antioxidant metabolism in Lemna minor L. Caryologia 60:125–128Google Scholar
  45. 45.
    Shi P, Chen GC, Huang ZW (2005) Effects of La3+ on the active oxygen scavenging enzyme activities in cucumber seedling leaves. Russ J Plant Physiol 52:294–297CrossRefGoogle Scholar
  46. 46.
    Yuan YJ, Li JC, Ge ZQ et al (2002) Superoxide anion burst and taxol production induced by Ce4+ in suspension cultures of Taxus cuspidata. J Mol Catal B: Enzym 18:251–260CrossRefGoogle Scholar
  47. 47.
    Wang YL, Wang XD, Zhao B et al (2007) Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements. Plant Growth Regul 52:151–159CrossRefGoogle Scholar
  48. 48.
    Camm EL, Towers GHN (1973) Review article: phenylalanine ammonia lyase. Phytochemistry 12:961–973CrossRefGoogle Scholar
  49. 49.
    Lister CE, Lancaster JE, Walker JRL (1996) Phenylalanine ammonia-lyase (PAL) activity and its relationship to anthocyanin and flavonoid levels in New Zealand-grown apple cultivars. J Am Soc Hortic Sci 12(2):281–285Google Scholar
  50. 50.
    Creasy LL (1971) Role of phenylalanine in the biosynthesis of flavonoids and cinnamic acids in strawberry leaf disks. Phytochemistry 10(11):2705–2711CrossRefGoogle Scholar
  51. 51.
    Eichholza I, Rohn S, Gamm A et al (2012) UV-B-mediated flavonoid synthesis in white asparagus (Asparagus offcinalis L.). Food Res Int 48:196–201CrossRefGoogle Scholar
  52. 52.
    Huang J, Gu M, Lai Z et al (2010) Functional analysis of the Arabidopsis PAL gene family in plant growth, development, and response to environmental stress. Plant Physiol 153(4):1526–1538PubMedCrossRefGoogle Scholar
  53. 53.
    Hossain Z, Mandal AK, Datta SK et al (2006) Development of NaCl tolerant strain in Chrysanthemum morifolium Ramat. through in vitro mutagenesis. Plant Biol 8(4):450–461PubMedCrossRefGoogle Scholar
  54. 54.
    Muarlidharan J, John E, Channamma L et al (1996) Change in esterases in response to blast infection in fingermillet seedlings. Phytochemistry 43:1151–1155CrossRefGoogle Scholar
  55. 55.
    Sahoo MR, Dasgupta M, Kole PC et al (2007) Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance. Mycopathologia 163(4):241–248PubMedCrossRefGoogle Scholar
  56. 56.
    Roy S, Begum Y, Chakraborty A et al (2006) Radiation-induced phenotypic alterations in relation to isozymes and RAPD markers in Vigna radiate (L) Wilczek. Int J Radiat Biol 82(11):823–832PubMedCrossRefGoogle Scholar
  57. 57.
    Bogdanovic J, Milosavic N, Prodanovic R et al (2007) Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). Biochem Syst Ecol 35:263–273CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Peng Xin
    • 1
  • Zhou Shuang-Lin
    • 1
  • He Jun-Yao
    • 1
  • Ding Li
    • 1
  1. 1.Zhejiang Pharmaceutical CollegeNingboChina

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