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Trace Element, Antioxidant Activity, and Lipid Peroxidation Levels in Brain Cortex of Gerbils After Cerebral Ischemic Injury

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Abstract

Proper trace element level and antioxidant enzyme activity are crucial for the brain in maintaining normal neurological functions. To our knowledge, alteration of lipid peroxidation status, trace element level, and antioxidant activity in the homogenates of brain cortex after cerebral ischemia in gerbil, however, has not been investigated so far. Male Mongolian gerbils were divided into control and ischemic subjects. Cerebral ischemia was induced by occlusion of the right middle cerebral artery and right common carotid artery for 1 h. Experimental results showed that a significant increase (P < 0.01) of the malondialdehyde level was found in the ischemic brain as compared with the control group. Trace element analysis indicated that a remarkable elevation (P < 0.01) of the level of iron (Fe), chromium (Cr), and a statistical decrease of selenium (Se) and zinc (Zn) (P < 0.05) concentration were observed in the ischemic brain as compared with the control subject. No significant change (P > 0.05) of the copper (Cu) level was found in both experimental groups. Additionally, antioxidant activity of superoxide dismutase (P < 0.01) and catalase (P < 0.05) was significantly decreased in the ischemic brain as compared with the control subject. Taking all results together, it is conceivable to manifest the experimental findings that cerebral ischemia not only may result in an enhanced oxidative stress but also may lead to further oxidative injury. Moreover, disturbance of trace element level combined with declined antioxidant activity seems to play a significant role in responsible for the etiology of cerebral ischemia.

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Acknowledgments

The authors gratefully acknowledge the National Science Council of Taiwan (NSC 99-2314-B-166-001-MY3) and Central Taiwan University of Science and Technology (CTU99-PC-012) for financial support.

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Authors and Affiliations

  1. Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, No.666, Buzih Road, Beitun District, Taichung, 406, Taiwan

    Kwang-Ming Fang

  2. Department of Medical Research, Taichung Veterans General Hospital, Taichung, 406, Taiwan

    Fu-Chou Cheng

  3. Department of Medical Laboratory Science and Biotechnology, Kaoshiung Medical University, Kaoshiung, 700, Taiwan

    Yeou-Lih Huang

  4. Department of Nursing, Central Taiwan University of Science and Technology, No.666, Buzih Road, Beitun District, Taichung, 406, Taiwan

    Shu-Ying Chung

  5. Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, No.666, Buzih Road, Beitun District, Taichung, 406, Taiwan

    Zhong-Yang Jian & Ming-Cheng Lin

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  1. Kwang-Ming Fang
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Correspondence to Ming-Cheng Lin.

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Fang, KM., Cheng, FC., Huang, YL. et al. Trace Element, Antioxidant Activity, and Lipid Peroxidation Levels in Brain Cortex of Gerbils After Cerebral Ischemic Injury. Biol Trace Elem Res 152, 66–74 (2013). https://doi.org/10.1007/s12011-012-9596-1

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