Biological Trace Element Research

, Volume 151, Issue 2, pp 234–239

Changes of the Serum Cytokine Contents in Broilers Fed on Diets Supplemented with Nickel Chloride

  • Bangyuan Wu
  • Hengmin Cui
  • Xi Peng
  • Jing Fang
  • Zhicai Zuo
  • Jianying Huang
  • Qin Luo
  • Yubing Deng
  • Hesong Wang
  • Juan Liu


Cytokines are immunoregulatory proteins which play an important role in the immune system. The purpose of this study was to examine the serum cytokine contents including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interferon gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) induced by dietary nickel chloride in broilers by enzyme-linked immunospecific assay. A total of 240 one-day-old avian broilers were divided into four groups and fed on a corn–soybean basal diet as control diet or the same basal diet supplemented with 300, 600, and 900 mg/kg of nickel chloride. During the experimental period of 42 days, the results showed that the serum IL-2, IL-4, IL-6, IL-10, IFN-γ and TNF-α contents were lower (p < 0.05 or p < 0.01) in the 300, 600, 900 mg/kg groups than those in the control group. It was concluded that dietary nickel chloride in the range of 300 to 900 mg/kg could reduce the serum cytokine contents, which could finally impact the immune function in broilers.


Nickel chloride IL-2 IL-4 IL-6 IL-10 IFN-γ TNF-α Serum Broiler 


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Bangyuan Wu
    • 1
  • Hengmin Cui
    • 1
    • 2
  • Xi Peng
    • 1
  • Jing Fang
    • 1
  • Zhicai Zuo
    • 1
  • Jianying Huang
    • 1
  • Qin Luo
    • 1
  • Yubing Deng
    • 1
  • Hesong Wang
    • 1
  • Juan Liu
    • 1
  1. 1.Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary MedicineSichuan Agricultural UniversityYa’anChina
  2. 2.College of Veterinary MedicineSichuan Agricultural UniversityYa’anChina

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