Biological Trace Element Research

, Volume 151, Issue 2, pp 307–314 | Cite as

The Probiotic Bacterial Strain Lactobacillus fermentum D3 Increases In Vitro the Bioavailability of Ca, P, and Zn in Fermented Goat Milk

  • Triana Bergillos-Meca
  • Miguel Navarro-Alarcón
  • Carmen Cabrera-Vique
  • Reyes Artacho
  • Manuel Olalla
  • Rafael Giménez
  • Miriam Moreno-Montoro
  • Alfonso Ruiz-Bravo
  • Agustín Lasserrot
  • Mª Dolores Ruiz-López


We determined calcium, magnesium, phosphorus and zinc levels in a total of 27 samples of commercial goat- and cow-milk fermented products and 9 samples of a goat-milk fermented product with addition of a probiotic bacterial strain, Lactobacillus fermentum D3, manufactured experimentally by our research group. Atomic absorption spectroscopy with flame atomization and UV/VIS spectrophotometry were used as analytic techniques. The results of an in vitro digestion process showed that the bioavailability of calcium, phosphorus, and zinc was significantly higher in our fermented milk containing the probiotic bacterial strain than it was in commercial goat-milk fermented products. Furthermore, our product showed a significantly higher bioavailability of calcium and zinc compared to goat- and cow-milk fermented products made with other microorganisms. We conclude that, in in vitro assays, strain D3 seems to increase the bioavailability of these minerals and that this new product may constitute a better source of bioavailable minerals compared to other products already on the market.


Fermented goat Cow-milk products Mineral composition Dialyzable fraction Probiotic starter culture 



This research was supported by grant AGR-4915 from the Andalusian regional government in Spain. Two of the authors (T Bergillos-Meca and M Moreno-Montoro) wish to thank the Andalusian regional government and Department of Education (FPU program) for the concession of their PhD fellowships.


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Triana Bergillos-Meca
    • 1
  • Miguel Navarro-Alarcón
    • 1
  • Carmen Cabrera-Vique
    • 1
  • Reyes Artacho
    • 1
  • Manuel Olalla
    • 1
  • Rafael Giménez
    • 1
  • Miriam Moreno-Montoro
    • 1
  • Alfonso Ruiz-Bravo
    • 2
  • Agustín Lasserrot
    • 3
  • Mª Dolores Ruiz-López
    • 1
    • 4
  1. 1.Dpto. de Nutrición y Bromatología, Facultad de FarmaciaUniversidad de Granada, Campus de CartujaGranadaSpain
  2. 2.Dpto. de Microbiología, Facultad de FarmaciaUniversidad de Granada, Campus de CartujaGranadaSpain
  3. 3.Empresa Biotmicrogen S.L., Campus Tecnológico de la SaludGranadaSpain
  4. 4.Instituto de Nutrición y Tecnología de los AlimentosUniversidad de Granada, Campus de CartujaGranadaSpain

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