Biological Trace Element Research

, Volume 151, Issue 1, pp 30–37 | Cite as

Mercury Concentration in Breast Milk and Infant Exposure Assessment During the First 90 Days of Lactation in a Midwestern Region of Brazil

  • Leandro Rodrigues da Cunha
  • Teresa Helena Macedo da Costa
  • Eloisa Dutra Caldas
Article

Abstract

Breast milk samples collected from 18 nursing mothers between the 15th and 90th day of lactation were digested in nitric acid in a microwave, and total mercury (THg) levels were quantified by atomic fluorescence spectrometry. Participants responded to a 24-h dietary recall questionnaire on the 74th and 76th day of lactation and to a Food Frequency Questionnaire querying the frequency of fish intake over the last 90 days. Usual intake was estimated using the PC-SIDE software package. A meal of fish was offered on the 75th day of lactation. Mothers’ individual mean THg levels ranged from <0.76 to 22.7 ng/mL during the period, and the mean level for all samples (n = 142) was 6.47 ±6.04 ng/mL. The multilevel mixed linear model used showed high heterogeneity of the mercury levels among the mothers, and THg levels did not change significantly over the period under study. However, a significant increase in THg levels was observed after the intervention with the fish meal. Exposure increased for most infants on the 90th day of lactation, with intakes exceeding the THg provisional tolerable weekly intake (PTWI) at least once during the period for 77.8 % of samples. Mothers consumed mostly food from the fat and grain groups, and a significant correlation was detected between consumption of food of these groups and breast milk THg levels (p = 0.006 and 0.007). A significant correlation was also found between vegetable consumption and carbohydrate intake and THg levels in the samples (p = 0.015 and 0.045, respectively). No correlation was found between mothers’ daily fish consumption frequency and THg levels. Although this study showed that mercury intake by infants during lactation may exceed the toxicologically safe exposure level (PTWI), we nevertheless believe that the benefits of lactation for both the mother and the infant outweigh the eventual risks that this exposure may represent.

Keywords

Mercury Breast milk Food consumption Infant exposure Brazil 

Notes

Acknowledgments

We would like to thank the CNPq (National Technological and Scientific Development Council) for providing a master’s scholarship to L.R. Cunha. We would also like to thank Dr. Alisia L. Curriquiry for providing access to PC-Side used in the dietary analysis and Prof. Jurandir R. de Souza for his support during mercury analysis at the Laboratory of Analytical and Environmental Chemistry. We greatly appreciate the suggestions and comments provided by Prof. Jose G. Dorea and the help of Prof. Eduardo Freitas da Silva with the statistical analysis.

References

  1. 1.
    Leon-Cava N, Lutter C, Ross J, Martin L (2002) Quantifying the benefits of breastfeeding: a summary of the evidence. Food and Nutrition Program (HPN) of the Division of Health Promotion and Protection (HPP) of the Pan American Health Organization (PAHO). Washington, D.C.: PAHO© 2002Google Scholar
  2. 2.
    Jones G, Steketee RW, Black RE, Bhutta ZA, Morris SS, Bellagio Child Survival Study Group (2003) How many child deaths can we prevent this year? Lancet 362:65–71PubMedCrossRefGoogle Scholar
  3. 3.
    Pettitt DJ, Forman MR, Hanson RL, Knowler WC, Bennett PH (1997) Breastfeeding and incidence of non-insulin-dependent diabetes mellitus in Pima Indians. Lancet 350:166–168PubMedCrossRefGoogle Scholar
  4. 4.
    Wilson AC, Forsyth JS, Greene SA, Irvine L, Hau C, Howie PW (1998) Relation of infant diet to childhood health: seven year follow up of cohort of children in Dundee infant feeding study. BMJ 316:21–25PubMedCrossRefGoogle Scholar
  5. 5.
    Martin RM, Ben-Shlomo Y, Gunnell D, Elwood P, Yarnell JW, Davey Smith G (2005) Breast feeding and cardiovascular disease risk factors, incidence and mortality: the Caerphilly study. J Epidemiol Comm Health 59:121–9CrossRefGoogle Scholar
  6. 6.
    Gina M, Solomon GM, Pilar M, Weiss PM (2002) Chemical contaminants in breast milk: time trends and regional variability. Environ Health Perspect 110:A339–A347CrossRefGoogle Scholar
  7. 7.
    World Health Organization (2008) Guidance for identifying populations at risk from mercury exposure. Issued by UNEP DTIE Chemicals Branch and WHO Department of Food Safety, Zoonoses and Foodborne Diseases, Geneva, Switzerland, 2008. At http://www.who.int/foodsafety/publications/chem/mercuryexposure.pdf. Acessed 09 Nov 2012
  8. 8.
    Lechlera PJ, Millerb JR, Lacerdac LD, Vinsond D, Bonzongoe J-C, Lyonsd WB, Warwicka JJ (2000) Elevated mercury concentrations in soils, sediments, water, and fish of the Madeira River basin, Brazilian Amazon: a function of natural enrichments? Sci Total Environ 260:87–96CrossRefGoogle Scholar
  9. 9.
    Berzas Nevado JJ, Rodrıguez Martın-Doimeadios RC, Guzman Bernardo FJ, Jimenez Moreno M, Herculano AM, do Nascimento JL et al (2010) Mercury in the Tapajos River basin, Brazilian Amazon: a review. Environ Int 36(6):593–608PubMedCrossRefGoogle Scholar
  10. 10.
    Seixas TG, Moreira I, Malm O, Kehrig HA (2012) Mercury and selenium in a top-predator fish, Trichiurus lepturus (Linnaeus, 1758), from the Tropical Brazilian Coast, Rio de Janeiro. Bull Environ Contam Toxicol 89:434–438PubMedCrossRefGoogle Scholar
  11. 11.
    Botaro D, Torres JP, Schramm KW, Malm O (2012) Mercury levels in feed and muscle of farmed tilapia. Am J Indust Med 55:1159–1165Google Scholar
  12. 12.
    Azevedo JS, Braga ES, Favaro DT, Perretti AR, Rezende CE, Souza CM (2011) Total mercury in sediments and in Brazilian Ariidae catfish from two estuaries under different anthropogenic influence. Mar Pollut Bull 62:2724–2731PubMedCrossRefGoogle Scholar
  13. 13.
    Malm O, Branches FJP, Akagi H, Castro MB, Pfeiffer WC, Haradac M, Bastosa WR, Katob H (1995) Mercury and methylmercury in fish and human hair from the Tapajós river basin, Brazil. Sci Total Environ 175:141–150PubMedCrossRefGoogle Scholar
  14. 14.
    Kehrig HA, Malm O, Akagi H, Guimaraes JRD, Torres JPM (1998) Methylmercury in fish and hair samples from the Balbina Reservoir, Brazilian Amazon. Environ Res 77:84–90PubMedCrossRefGoogle Scholar
  15. 15.
    Grandjean P, Weihe P, White RF, Debes F, Araki S, Yokoyama K, Murata K, Sørensen N, Dahl R, Jørgensen PJ (1997) Cognitive deficit in 7-year-old children with prenatal exposure to methylmercury. Neurotoxicol Teratol 19:417–28PubMedCrossRefGoogle Scholar
  16. 16.
    Jensen TK, Grandjean P, Jørgensen EB, White RF, Debes F, Weihe P (2005) Effects of breast feeding on neuropsychological development in a community with methylmercury exposure from seafood. J Expo Anal Environ Epidemiol 15:423–30PubMedCrossRefGoogle Scholar
  17. 17.
    Castoldi AF, Johansson C, Onishchenko N, Coccini T, Roda E, Vahter M, Ceccatelli S, Manzo L (2008) Human developmental neurotoxicity of methylmercury: impact of variables and risk modifiers. Regul Toxicol Pharmacol 51:201–214PubMedCrossRefGoogle Scholar
  18. 18.
    World Health Organization (2004) WHO Technical Report Series. Methylmercury. Sixty-first Report of the Joint FAO/WHO Expert Committee on Food Additives (JECFA). Geneva, Switzerland, 2004. At http://whqlibdoc.who.int/trs/WHO_TRS_922.pdf. Acessed 09 Nov 2012
  19. 19.
    IOM (2005) Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids (macronutrients), Institute of Medicine. At http://www.nap.edu/catalog.php?record_id=10490. Acessed 09 Nov 2012
  20. 20.
    Daniels JL, Longnecker MP, Rowland AS, Golding J, ALSPAC Study Team, University of Bristol Institute of Child Health (2004) Fish intake during pregnancy and early cognitive development of offspring. Epidemiology 15:394–402PubMedCrossRefGoogle Scholar
  21. 21.
    Fox DA, Grandjean P, de Groot D, Paule MG (2012) Developmental origins of adult diseases and neurotoxicity: epidemiological and experimental studies. Neurotoxicology 33:810–816PubMedCrossRefGoogle Scholar
  22. 22.
    Gundacker C, Pietschnig B, Wittmann KJ, Lischka A, Salzer H, Hohenauer L, Schuster E (2002) Lead and mercury in breast milk. Pediatrics 110:873–878PubMedCrossRefGoogle Scholar
  23. 23.
    Al-Saleh K, Shinwari N, Mashhour A (2003) Heavy metal concentrations in the breast milk of Saudi women. Biol Trace Elem Res 96:21–37PubMedCrossRefGoogle Scholar
  24. 24.
    da Costa SL, Malm O, Dorea JG (2005) Breast-milk mercury concentrations and amalgam surface in mothers from Brasília, Brazil. Biol Trace Element Res 106:145–151CrossRefGoogle Scholar
  25. 25.
    Yalçin SS, Yurdakök K, Yalçin S, Engür-Karasimav D, Coşkun T (2010) Maternal and environmental determinants of breast-milk mercury concentrations. Turk J Ped 52:1–9Google Scholar
  26. 26.
    García-Esquinas E, Pérez-Gómez B, Fernández MA, Pérez-Meixeira AM, Gil E, de Paz C, Iriso A, Sanz JC, Astray J, Cisneros M, de Santos A, Asensio A, García-Sagredo JM et al (2011) Mercury, lead and cadmium in human milk in relation to diet, lifestyle habits and sociodemographic variables in Madrid (Spain). Chemosphere 85:268–276PubMedCrossRefGoogle Scholar
  27. 27.
    Grandjean P, Weihe P, Needham LL, Burse VW, Patterson DG Jr, Sampson EJ, Jørgensen PJ, Vahter M (1995) Relation of a seafood diet to mercury, selenium, arsenic, and polychlorinated biphenyl and other organochlorine concentrations in human milk. Environ Res 71:29–38PubMedCrossRefGoogle Scholar
  28. 28.
    Lohman TG, Roche AF, Martorell R (1988) Anthropometric standardization reference manual. Human Kinetics Books, ChampaignGoogle Scholar
  29. 29.
    Mills A, Tyler H (1992) Food and nutrient intakes of British infants aged 6–12 months. HMSO, LondonGoogle Scholar
  30. 30.
    da Costa THM, Haisma H, Wells JCK, Mander AP, Whitehead RG, Bluck LJC (2010) How much human milk do infants consume? Data from 12 countries using a standardized stable isotope methodology. J Nutr 140:2027–2032Google Scholar
  31. 31.
    Nusser SM, Carriquiry AL, Dodd KW, Fuller WA (1996) A semiparametric transformation approach to estimating usual daily intake distributions. J Am Stat Assoc 91:1440–1449CrossRefGoogle Scholar
  32. 32.
    Barbosa AC, Dórea JG (1998) Indices of mercury contamination during breast feeding in the Amazon Basin. Environ Toxicol Pharmacol 6:71–79PubMedCrossRefGoogle Scholar
  33. 33.
    Boischio AA, Henshel DS (2000) Linear regression models of methyl mercury exposure during prenatal and early postnatal life among riverside people along the upper Madeira river, Amazon. Environ Res 83:150–161PubMedCrossRefGoogle Scholar
  34. 34.
    Miklavcic A, Cuderman P, Mazej D, Tratnik JS, Krsnik M, Planinsek P, Osredkar J, Horvat M (2011) Biomarkers of low-level mercury exposure through fish consumption in pregnant and lactating Slovenian women. Environ Res 111:1201–1207PubMedCrossRefGoogle Scholar
  35. 35.
    Oskarsson A, Schültz A, Skerfving S, Hallén IP, Ohlin B, Lagerkvist BJ (1996) Total and inorganic mercury in breast milk in relation to fish consumption and amalgam in lactating women. Arch Environ Health 51:234–41PubMedCrossRefGoogle Scholar
  36. 36.
    Dorea JG (2004) Mercury and lead during breast-feeding. Brit J Nut 92:21–40CrossRefGoogle Scholar
  37. 37.
    Drexler H, Schaller KH (1998) The mercury concentration in breast milk resulting from amalgam fillings and dietary habits. Envirom Res 77:124–129CrossRefGoogle Scholar
  38. 38.
    Björnberg KA, Vahter M, Berglung B, Niklasson B, Blennow M, Englung GS (2005) Transport of methylmercury and inorganic mercury to the fetus and breast-fed infant. Environ Health Perspect 113:1381–1385PubMedCrossRefGoogle Scholar
  39. 39.
    Chapman L, Chan HM (2000) The influence of nutrition on methyl mercury intoxication. Environ Health Perspect 108(suppl 1):29–36PubMedGoogle Scholar
  40. 40.
    Passos CJS, Mergler D, Fillion M, Lemire M, Mertens F, Guimarães JRD, Philibert A (2007) Epidemiologic confirmation that fruit consumption influences mercury exposure in the Brazilian Amazon. Environ Res 105:183–193PubMedCrossRefGoogle Scholar
  41. 41.
    Kwon YM, Lee HS, Yoo DC, Kim CH, Kim GS, Kim JA, Lee YN, Kim YS, Kang KM, No KM, Paek OJ, Seo JH, Choi H, Park SK, Choi DM, Kim DS, Choi DW (2009) Dietary exposure and risk assessment of mercury from the Korean total diet study. J Toxicol Environ Health A72:1484–1492CrossRefGoogle Scholar
  42. 42.
    Meng B, Feng X, Qiu G, Cai Y, Wang D, Li P, Shang L, Sommar J (2010) Distribution patterns of inorganic mercury and methylmercury in tissues of rice (Oryza sativa L.) plants and possible bioaccumulation pathways. J Agric Food Chem 58:4951–4958PubMedCrossRefGoogle Scholar
  43. 43.
    Chen C, Qian Y, Chen Q, Li C (2011) Assessment of daily intake of toxic elements due to consumption of vegetables, fruits, meat, and seafood by inhabitants of Xiamen, China. J Food Sci 76:T181–188PubMedCrossRefGoogle Scholar
  44. 44.
    Behrooz RD, Esmaili-Sari A, Peer FE, Amini M (2012) Mercury concentration in the breast milk of Iranian women. Biol Trace Elem Res 147:36–43PubMedCrossRefGoogle Scholar
  45. 45.
    Sakamoto M, Kubota M, Matsumoto S, Nakano A, Akag H (2002) Declining risk of methylmercury exposure to infants during lactation. Environ Res 90:185–189PubMedCrossRefGoogle Scholar
  46. 46.
    Myers GJ, Thurston SW, Pearson AT, Davidson PW, Cox C, Shamlaye CF, Cernichiari E, Clarkson TW (2009) Postnatal exposure to methyl mercury from fish consumption: a review and new data from the Seychelles Child Development Study. Neurotoxicology 30:338–49PubMedCrossRefGoogle Scholar
  47. 47.
    Grotto D, Valentini J, Fillion M, Passos CJ, Garcia SC, Mergler D, Barbosa F Jr (2010) Mercury exposure and oxidative stress in communities of the Brazilian Amazon. Sci Total Environ 408:806–811PubMedCrossRefGoogle Scholar
  48. 48.
    US FDA (2004) What you need to know about mercury in fish and shellfish. US Food and Drugs Adminsitration; US Environmental Protection Agency. At http://www.fda.gov/downloads/Food/ResourcesForYou/Consumers/UCM182158.pdf. Acessed 09 Nov 2012
  49. 49.
    Lando AM, Fein SB, Choinière CJ (2012) Awareness of methylmercury in fish and fish consumption among pregnant and postpartum women and women of childbearing age in the United States. Environ Res 116:85–92PubMedCrossRefGoogle Scholar
  50. 50.
    IBGE (Brazilian Institute of Geography and Statistics) (2012) Pesquisa de Orçamentos Familiares: 2003–2004 and 2008–2009. IBGE: Rio de Janeiro, Brazil. At http://www.ibge.gov.br/home/mapa_site/mapa_site.php#populacao. Acessed 09 Nov 2012

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Leandro Rodrigues da Cunha
    • 1
  • Teresa Helena Macedo da Costa
    • 2
  • Eloisa Dutra Caldas
    • 1
  1. 1.Laboratory of Toxicology, Faculty of Health ScienceUniversity of BrasiliaBrasiliaBrazil
  2. 2.Department of Nutrition, Faculty of Health ScienceUniversity of BrasiliaBrasiliaBrazil

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