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Biological Trace Element Research

, Volume 150, Issue 1–3, pp 433–440 | Cite as

The Dual-Effects of LaCl3 on the Proliferation, Osteogenic Differentiation, and Mineralization of MC3T3-E1 Cells

  • Dandan Liu
  • Jinchao Zhang
  • Guifang Wang
  • Xiaolong Liu
  • Shuxiang Wang
  • MengSu Yang
Article

Abstract

A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase (ALP) activity measurement, alizarin red S stain and measurement, quantitative real-time reverse transcriptase polymerase chain reaction, and Western blot analysis were employed to assess the effects of LaCl3 on the proliferation, osteogenic differentiation, and mineralization of a murine preosteoblast cell line MC3T3-E1 at cell and molecular levels. The results indicated that LaCl3 had dual effects on the proliferation, osteogenic differentiation, and mineralization of MC3T3-E1 cells. First, LaCl3 promoted the proliferation, osteogenic differentiation, and mineralization of MC3T3-E1 cells at lower concentrations, then had no effects and further turned to inhibit the proliferation, osteogenic differentiation, and mineralization of MC3T3-E1 cells with increasing concentrations. The expression of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP2), ALP, bone sialoprotein (BSP), collagen I (Col I), and osteocalcin (OCN) genes was upregulated in the presence of 0.0001 and 0.1 μM LaCl3, but these genes were downregulated in the MC3T3-E1 cells treated with 1,000 μM LaCl3. In addition, the expression of BMP2, Runx2, and OCN proteins was promoted by LaCl3 at the concentration of 0.0001 μM, but these proteins were downregulated after 1,000 μM LaCl3 treatment. The results suggest that LaCl3 likely up- or downregulates the expression of Runx2, which subsequently up- or downregulates osteoblasts marker genes Col I and BMP2 at early stages and ALP and OCN at later stages of differentiation, thus causes to promote or inhibit the proliferation, osteogenic differentiation and mineralization of MC3T3-E1 cells. The results will be helpful for understanding the mechanisms of bone metabolism and application of lanthanum-based compounds in the future.

Keywords

Lanthanum Proliferation Osteogenic differentiation Mineralization 

Abbreviations

ARS

Alizarin red S

ALP

Alkaline phosphatase

α-MEM

Alpha minimum essential medium

BMP2

Bone morphogenetic protein 2

BSP

Bone sialoprotein

Col I

Collagen I

cDNA

Complementary DNA

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

ECL

Enhanced chemiluminescene

EDTA

Ethylenediaminetetraacetic acid tetrasodium salt

FBS

Fetal bovine serum

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

La

Lanthanum

OD

Optical density

OBs

Osteoblasts

OCN

Osteocalcin

OS

Osteogenetic induction supplement

Q-PCR

Quantitative real-time reverse transcriptase polymerase chain reaction

Runx2

Runt-related transcription factor 2

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 20971034), Natural Science Key Foundation of Hebei Province (no. B2009000161), Research Fund for the Doctoral Program of Higher Education of China (no.20111301110004).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Dandan Liu
    • 1
  • Jinchao Zhang
    • 1
  • Guifang Wang
    • 2
  • Xiaolong Liu
    • 1
  • Shuxiang Wang
    • 1
  • MengSu Yang
    • 3
  1. 1.College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei ProvinceHebei UniversityBaodingPeople’s Republic of China
  2. 2.School of MedicineHebei UniversityBaodingPeople’s Republic of China
  3. 3.Department of Biology and ChemistryCity University of Hong KongHong KongChina

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