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Biological Trace Element Research

, Volume 150, Issue 1–3, pp 424–432 | Cite as

Zinc Inhibits High Glucose-Induced Apoptosis in Peritoneal Mesothelial Cells

  • Xiuli Zhang
  • Dan Liang
  • Baolei Guo
  • Lina Yang
  • Lining Wang
  • Jianfei MaEmail author
Article

Abstract

Zinc (Zn) plays an important role in influencing many types of apoptosis. However, its function in apoptosis in peritoneal mesothelial cells (PMCs) remains unknown. Here, we studied the effects of Zn on high glucose (HG)-induced apoptosis in rat PMCs (RPMCs) and examined the underlying molecular mechanisms. We found that Zn supplementation inhibited HG-induced RPMC apoptosis significantly, by attenuating reactive oxygen species (ROS) production, inhibiting HG-induced sFasR and sFasL over-expression, caspase-8 and caspase-3 activation, and inhibiting release of cytochrome c from mitochondria to the cytosol. Further analysis revealed that Zn supplementation facilitated cell survival through activation of the phosphatidylinositol 3-kinase/Akt signaling pathway and MAPK/ERK pathways. These results indicate that Zn can inhibit apoptosis in HG-induced RPMCs by several independent mechanisms, including an indirect antioxidative effect and probably by inhibition of caspase-8 and caspase-3 activation.

Keywords

Rat peritoneal mesothelial cells Zinc High glucose Apoptosis 

Abbreviations

RPMCs

Rat peritoneal mesothelial cells

HPMCs

Human peritoneal mesothelial cells

HG

High glucose

CAPD

Continuous ambulatory peritoneal dialysis

DMEM

Dulbecco’s modified Eagle’s medium

FITC

Fluorescein isothiocyanate

TPEN

N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine

RIPA

Radioimmune precipitation assay

TGF

Transforming growth factor

PBS

Phosphate-buffered saline

DCF-DA

2,7-Dichlorofluorescein-diacetate

TBS

Tris-buffered saline

PDF

Peritoneal dialysis fluid

TNF-α

Tumor necrosis factor-α

MAPK

Mitogen-activated protein kinase

ERK

Extracellular-signal-regulated kinase

PI

Propidium iodide

ROS

Reactive oxygen species

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xiuli Zhang
    • 1
    • 2
  • Dan Liang
    • 3
    • 4
  • Baolei Guo
    • 3
  • Lina Yang
    • 1
  • Lining Wang
    • 1
  • Jianfei Ma
    • 1
    Email author
  1. 1.Department of NephrologyThe First Affiliated Hospital of China Medical UniversityShenyangPeople’s Republic of China
  2. 2.Department of NephrologyBenxi Railway HospitalBenxiPeople’s Republic of China
  3. 3.Department of OrthopedicsThe First Affiliated Hospital of China Medical UniversityShenyangPeople’s Republic of China
  4. 4.Troops of 95935 unitHaerbinPeople’s Republic of China

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