Biological Trace Element Research

, Volume 149, Issue 1, pp 29–33

Matrix Metalloproteinase-9 and the Cu/Zn Ratio as Ancillary Diagnostic Tools in Distinguishing Between the Classical and Follicular Variants of Papillary Thyroid Carcinoma

  • Vesna V. Dragutinović
  • Svetislav B. Tatić
  • Snežana Nikolić-Mandić
  • Svetlana Savin
  • Dubravka Cvejić
  • Duško Dunđerović
  • Milan Gajić
  • Ivan Paunović
Article

DOI: 10.1007/s12011-012-9404-y

Cite this article as:
Dragutinović, V.V., Tatić, S.B., Nikolić-Mandić, S. et al. Biol Trace Elem Res (2012) 149: 29. doi:10.1007/s12011-012-9404-y

Abstract

The most common histological variants of papillary thyroid carcinoma (PTC), classical (CPTC) and follicular (FPTC), have different diagnostic features, molecular biology, and prognosis. Matrix metalloproteinase-9 (MMP-9) endopeptidase which degrades the components of the extracellular matrix is essential in the invasive growth and metastasizing of malignant tumors. The serum copper (Cu)/zinc (Zn) ratios are sensitive diagnostic and prognostic indicators in oncology since Cu- and Zn-dependent enzymes play important roles in the genesis and the progression of tumors. The aim of this study was to examine the expressions of MMP-9 in tissues of CPTC and FPTC, as well as to determine the Cu/Zn ratios in the same samples. MMP-9 was determined immunohistochemically, and the concentrations of copper and zinc in thyroid tissue were determined by means of flame atomic absorption spectrometry. The results obtained revealed significantly higher expressions of MMP-9 in CPTC in comparison with FPTC, as well as higher Cu/Zn ratios in CPTC than in FPTC. Thus, determining MMP-9 activities and the Cu/Zn ratios could improve the accuracy of the standard histopathological diagnosis of these two types of PTC.

Keywords

Papillary thyroid carcinoma Matrix metalloproteinase-9 Trace elements (Cu and Zn) Immunohistochemistry 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Vesna V. Dragutinović
    • 1
  • Svetislav B. Tatić
    • 2
  • Snežana Nikolić-Mandić
    • 3
  • Svetlana Savin
    • 4
  • Dubravka Cvejić
    • 4
  • Duško Dunđerović
    • 2
  • Milan Gajić
    • 5
  • Ivan Paunović
    • 6
    • 7
  1. 1.Institute of Medical Chemistry, Faculty of MedicineUniversity of BelgradeBelgradeSerbia
  2. 2.Institute of Pathology, Faculty of MedicineUniversity of BelgradeBelgradeSerbia
  3. 3.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  4. 4.Institute for the Application of Nuclear Energy–INEPUniversity of BelgradeBelgradeSerbia
  5. 5.Institute for Medical Statistics and Informatics, Faculty of MedicineUniversity of BelgradeBelgradeSerbia
  6. 6.Center for Endocrine Surgery, Clinic for Endocrinology, Diabetes and Diseases of MetabolismClinical Center of SerbiaBelgradeSerbia
  7. 7.Faculty of MedicineUniversity of BelgradeBelgradeSerbia

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