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Biological Trace Element Research

, Volume 148, Issue 3, pp 363–370 | Cite as

Flaxseed Oil as a Neuroprotective Agent on Lead Acetate-Induced Monoamineric Alterations and Neurotoxicity in Rats

  • Ahmed E. Abdel Moneim
Article

Abstract

Lead remains a considerable occupational and public health problem, which is known to cause a number of adverse effects in both man and animals. Here, the neuroprotective effect of flaxseed oil (1,000 mg/kg) on lead acetate (20 mg/kg) induced alternation in monoamines and brain oxidative stress was examined in rats. The levels of lead, dopamine (DA), norepinephrine (NE), serotonin (5-HT), lipid peroxidation, nitrite/nitrate (NO), and glutathione (GSH) were determined; also, the activity of acetylcholinesterase (AChE) and Na+-K+-ATPase were estimated on different brain regions of adult male albino rats. The level of lead was markedly elevated in different brain regions of rats. This leads to enhancement of lipid peroxidation and NO production in brain with concomitant reduction in AChE activity and GSH level. In addition, the levels of DA, NE, and 5-HT were decreased in the brain. These findings were associated with BAX over expression. Treatment of rats with flaxseed oil induced a marked improvement in most of the studied parameters as well as the immunohistochemistry features. These data indicated that dietary flaxseed oil provide protection against lead-induced oxidative stress and neurotoxic effects.

Keywords

Lead acetate Flaxseed oil Monoamines Acetylcholinesterase Oxidative stress Rats 

References

  1. 1.
    Flora SJ, Saxena G, Mehta A (2007) Reversal of lead-induced neuronal apoptosis by chelation treatment in rats: Role of reactive oxygen species and intracellular ca(2+). J Pharmacol Exp Ther 322(1):108–116PubMedCrossRefGoogle Scholar
  2. 2.
    Verina T, Rohde CA, Guilarte TR (2007) Environmental lead exposure during early life alters granule cell neurogenesis and morphology in the hippocampus of young adult rats. Neuroscience 145(3):1037–1047PubMedCrossRefGoogle Scholar
  3. 3.
    Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater 194:250–255PubMedCrossRefGoogle Scholar
  4. 4.
    Abdel-Moneim AE, Dkhil MA, Al-Quraishy S (2011) The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity. Biol Trace Elem Res 143(1):457–467PubMedCrossRefGoogle Scholar
  5. 5.
    Saxena G, Pathak U, Flora SJ (2005) Beneficial role of monoesters of meso-2,3-dimercaptosuccinic acid in the mobilization of lead and recovery of tissue oxidative injury in rats. Toxicology 214(1–2):39–56PubMedCrossRefGoogle Scholar
  6. 6.
    Kharoubi O, Slimani M, Aoues A (2011) Neuroprotective effect of wormwood against lead exposure. J Emerg Trauma Shock 4(1):82–88PubMedCrossRefGoogle Scholar
  7. 7.
    Devi CB, Reddy GH, Prasanthi RP, Chetty CS, Reddy GR (2005) Developmental lead exposure alters mitochondrial monoamine oxidase and synaptosomal catecholamine levels in rat brain. Int J Dev Neurosci 23(4):375–381PubMedCrossRefGoogle Scholar
  8. 8.
    Dąbrowska-Bouta B, Struzyńska L, Rafałowska U (1996) Does lead provoke the peroxidation process in rat brain synaptosomes? Mol Chem Neuropathol 29(2):127–139PubMedCrossRefGoogle Scholar
  9. 9.
    Gietzen DW, Woolley DE (1984) Acetylcholinesterase activity in the brain of rat pups and dams after exposure to lead via the maternal water supply. Neurotoxicology 5(3):235–246PubMedGoogle Scholar
  10. 10.
    Reddy GR, Basha MR, Devi CB, Suresh A, Baker JL, Shafeek A, Heinz J, Chetty CS (2003) Lead induced effects on acetylcholinesterase activity in cerebellum and hippocampus of developing rat. Int J Dev Neurosci 21(6):347–352PubMedCrossRefGoogle Scholar
  11. 11.
    Lawton LJ, Donaldson WE (1991) Lead-induced tissue fatty acid alterations and lipid peroxidation. Biol Trace Elem Res 28(2):83–97PubMedCrossRefGoogle Scholar
  12. 12.
    Ercal N, Treeratphan P, Hammond TC, Matthews RH, Grannemann NH, Spitz DR (1996) In vivo indices of oxidative stress in lead-exposed c57bl/6 mice are reduced by treatment with meso-2,3-dimercaptosuccinic acid or n-acetylcysteine. Free Radic Biol Med 21(2):157–161PubMedCrossRefGoogle Scholar
  13. 13.
    Uauy R, Dangour AD (2006) Nutrition in brain development and aging: role of essential fatty acids. Nutr Rev 64(5 Pt 2):S24–S33, discussion S72-91PubMedCrossRefGoogle Scholar
  14. 14.
    Bennett CN, Horrobin DF (2000) Gene targets related to phospholipid and fatty acid metabolism in schizophrenia and other psychiatric disorders: An update. Prostaglandins Leukot Essent Fatty Acids 63(1–2):47–59PubMedCrossRefGoogle Scholar
  15. 15.
    Seung Kim HF, Weeber EJ, Sweatt JD, Stoll AL, Marangell LB (2001) Inhibitory effects of omega-3 fatty acids on protein kinase c activity in vitro. Mol Psychiatry 6(2):246–248PubMedCrossRefGoogle Scholar
  16. 16.
    Itokazu N, Ikegaya Y, Nishikawa M, Matsuki N (2000) Bidirectional actions of docosahexaenoic acid on hippocampal neurotransmissions in vivo. Brain Res 862(1–2):211–216PubMedCrossRefGoogle Scholar
  17. 17.
    Innis SM (2000) The role of dietary n-6 and n-3 fatty acids in the developing brain. Dev Neurosci 22(5–6):474–480PubMedCrossRefGoogle Scholar
  18. 18.
    Ikemoto A, Nitta A, Furukawa S, Ohishi M, Nakamura A, Fujii Y, Okuyama H (2000) Dietary n-3 fatty acid deficiency decreases nerve growth factor content in rat hippocampus. Neurosci Lett 285(2):99–102PubMedCrossRefGoogle Scholar
  19. 19.
    Tsukada H, Kakiuchi T, Fukumoto D, Nishiyama S, Koga K (2000) Docosahexaenoic acid (dha) improves the age-related impairment of the coupling mechanism between neuronal activation and functional cerebral blood flow response: A pet study in conscious monkeys. Brain Res 862(1–2):180–186PubMedCrossRefGoogle Scholar
  20. 20.
    Moyad MA (2005) An introduction to dietary/supplemental omega-3 fatty acids for general health and prevention: Part ii. Urol Oncol 23(1):36–48PubMedCrossRefGoogle Scholar
  21. 21.
    Ito Y, Niiya Y, Kurita H, Shima S, Sarai S (1985) Serum lipid peroxide level and blood superoxide dismutase activity in workers with occupational exposure to lead. Int Arch Occup Environ Health 56(2):119–127PubMedCrossRefGoogle Scholar
  22. 22.
    Dabrowska-Bouta B, Struzynska L, Rafalowska U (1996) Effect of acute and chronic lead exposure on the level of sulfhydryl groups in rat brain. Acta Neurobiol Exp (Wars) 56(1):233–236Google Scholar
  23. 23.
    Dabrowska-Bouta B, Struzynska L, Rafalowska U (1996) Does lead provoke the peroxidation process in rat brain synaptosomes? Mol Chem Neuropathol 29(2–3):127–139PubMedCrossRefGoogle Scholar
  24. 24.
    Nowak P, Szczerbak G, Nitka D, Kostrzewa RM, Sitkiewicz T, Brus R (2008) Effect of prenatal lead exposure on nigrostriatal neurotransmission and hydroxyl radical formation in rat neostriatum: dopaminergic-nitrergic interaction. Toxicology 246(1):83–89PubMedCrossRefGoogle Scholar
  25. 25.
    Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) Effects of flaxseed oil on lead acetate-induced neurotoxicity in rats. Biol Trace Elem Res 144(1–3):904–913PubMedCrossRefGoogle Scholar
  26. 26.
    Glowinski J, Axelrod J, Iversen LL (1966) Regional studies of catecholamines in the rat brain. Iv. Effects of drugs on the disposition and metabolism of H3-norepinephrine and H3-dopamine. J Pharmacol Exp Ther 153(1):30–41PubMedGoogle Scholar
  27. 27.
    Tsakiris S, Schulpis KH, Marinou K, Behrakis P (2004) Protective effect of l-cysteine and glutathione on the modulated suckling rat brain Na+, K+, -ATPase and Mg2+ -ATPase activities induced by the in vitro galactosaemia. Pharmacol Res 49(5):475–479PubMedCrossRefGoogle Scholar
  28. 28.
    Jones DT, Hopkin SP (1998) Reduced survival and body size in the terrestrial isopod porcellio scaber from a metal-polluted environment. Environ Pollut 99(2):215–223PubMedCrossRefGoogle Scholar
  29. 29.
    Del Rosario AR, Guirguis GN, Perez GP, Matias VC, Li TH, Flessel CP (1982) A rapid and precise system for lead determination in whole blood. Int J Environ Anal Chem 12(3–4):223–231PubMedCrossRefGoogle Scholar
  30. 30.
    Carles J (1956) Colorimetric microdetermination of phosphorus. Bull Soc Chim Biol (Paris) 38(1):255–257Google Scholar
  31. 31.
    Ciarlone A (1978) Further modification of a fluoromertric method for analyzing brain amines. Microchem J 23(1):9–12CrossRefGoogle Scholar
  32. 32.
    Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95PubMedCrossRefGoogle Scholar
  33. 33.
    Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358PubMedCrossRefGoogle Scholar
  34. 34.
    Berkels R, Purol-Schnabel S, Roesen R (2004) Measurement of nitric oxide by reconversion of nitrate/nitrite to no. Methods Mol Biol 279:1–8PubMedGoogle Scholar
  35. 35.
    Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77PubMedCrossRefGoogle Scholar
  36. 36.
    Pedrycz A, Czerny K (2008) Immunohistochemical study of proteins linked to apoptosis in rat fetal kidney cells following prepregnancy adriamycin administration in the mother. Acta Histochem 110(6):519–523PubMedCrossRefGoogle Scholar
  37. 37.
    Jaya Prasanthi RP, Hariprasad Reddy G, Bhuvaneswari Devi C, Rajarami Reddy G (2005) Zinc and calcium reduce lead induced perturbations in the aminergic system of developing brain. Biometals 18(6):615–626PubMedCrossRefGoogle Scholar
  38. 38.
    Sidhu P, Nehru B (2003) Relationship between lead-induced biochemical and behavioral changes with trace element concentrations in rat brain. Biol Trace Elem Res 92(3):245–256PubMedCrossRefGoogle Scholar
  39. 39.
    Bagchi D, Vuchetich PJ, Bagchi M, Hassoun EA, Tran MX, Tang L, Stohs SJ (1997) Induction of oxidative stress by chronic administration of sodium dichromate [chromium vi] and cadmium chloride [cadmium ii] to rats. Free Radic Biol Med 22(3):471–478PubMedCrossRefGoogle Scholar
  40. 40.
    Basha MR, Wei W, Brydie M, Razmiafshari M, Zawia NH (2003) Lead-induced developmental perturbations in hippocampal sp1 DNA-binding are prevented by zinc supplementation: in vivo evidence for pb and zn competition. Int J Dev Neurosci 21(1):1–12PubMedCrossRefGoogle Scholar
  41. 41.
    Winder C, Kitchen I (1984) Lead neurotoxicity: a review of the biochemical, neurochemical and drug induced behavioural evidence. Prog Neurobiol 22(1):59–87PubMedCrossRefGoogle Scholar
  42. 42.
    du Bois TM, Deng C, Bell W, Huang XF (2006) Fatty acids differentially affect serotonin receptor and transporter binding in the rat brain. Neuroscience 139(4):1397–1403PubMedCrossRefGoogle Scholar
  43. 43.
    Logan AC (2003) Neurobehavioral aspects of omega-3 fatty acids: possible mechanisms and therapeutic value in major depression. Altern Med Rev 8(4):410–425PubMedGoogle Scholar
  44. 44.
    Yehuda S, Rabinovitz S, Mostofsky DI (2006) Nutritional deficiencies in learning and cognition. J Pediatr Gastroenterol Nutr 43(Suppl 3):S22–S25PubMedCrossRefGoogle Scholar
  45. 45.
    Prasad K (2005) Hypocholesterolemic and antiatherosclerotic effect of flax lignan complex isolated from flaxseed. Atherosclerosis 179(2):269–275PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Instituto de Biotecnología, Centro de Investigación Biomédica, Parque Tecnológico de Ciencias de la SaludUniversidad de GranadaGranadaSpain
  2. 2.Department of Zoology and Entomology, Faculty of ScienceHelwan UniversityCairoEgypt

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