Biological Trace Element Research

, Volume 148, Issue 1, pp 11–17 | Cite as

The Correlation Between Smoking Status of Family Members and Concentrations of Toxic Trace Elements in the Hair of Children

  • Muhittin A. SerdarEmail author
  • Beril S. Akin
  • Cem Razi
  • Okhan Akin
  • Serhat Tokgoz
  • Levent Kenar
  • Osman Aykut


Hair analysis is a promising tool for routine clinical screening and diagnosis of heavy metal exposure and essential trace element status in the human body. Systemic intoxications have been identified by anomalously high values of toxins in hair samples. The aim of the present study is to evaluate the relationship between smoking habit of the family members and the levels of toxic and nontoxic trace elements in hair samples of children. The randomized cross-sectional controlled study comprised of 95 children (41 girls and 54 boys) between the ages of 1 and 6 years. After written informed consent was obtained, a face-to-face interview was conducted with the families about educational background, total income of the family, and smoking habits of family members. The mineral elements considered in this study were Zn, Se, B, V, Co, Mo, Mn, iron (Fe), Be, aluminum (Al), As, cadmium (Cd), lead (Pb), Hg, chromium (Cr), Ag, Be, nickel (Ni), copper (Cu), Sn, and antimony (Sb). Hair mineral contents were measured by inductively coupled plasma–mass spectrometry. The results showed that the levels of Cd, Pb, Cr, Sb, Fe, and Al in hair samples of children whose parents smoked were significantly higher than those whose parents were nonsmokers. The number of smokers and the frequency of smoking at home were positively correlated with Pb, Cd, Cu, Ni, and Sb levels found. At the same time, it was found that there was no correlation between toxic element concentrations and family income or educational background excluding the levels of Cd. A correlation was observed between the smoking status of family members and levels of toxic trace elements in hair where this correlation was more significant with the levels of Pb and Cd. High socioeconomic status and the level of education of family members did not have any effect on toxic trace levels in hair samples of children.


Toxic and essential elements Hair analysis Smoking 


  1. 1.
    Suzuki T (1988) Hair and nails: advantages and pitfalls when used in biological monitoring. In: Clarkson TW, Friberg L, Nordberg GF, Sager PR (eds) Biological monitoring of toxic metals. Plenum Press, New York, pp 623–641CrossRefGoogle Scholar
  2. 2.
    Chłopicka J, Zagrodzki P, Zachwieja Z, Krośniak M, Fołta M (1995) Use of pattern recognition methods in the interpretation of heavy metal content (lead and cadmium) in children’s scalp hair. Analyst 120:943–945PubMedCrossRefGoogle Scholar
  3. 3.
    Teresa M, Vasconcelos SD, Tavares HMF (1997) Trace element concentrations in blood and hair of young apprentices of a technical-professional school. Sci Total Environ 205:189–199PubMedCrossRefGoogle Scholar
  4. 4.
    Northrop-Clewes CA, David I, Thurnham DI (2007) Monitoring micronutrients in cigarette smokers. Clin Chim Acta 377:14–38PubMedCrossRefGoogle Scholar
  5. 5.
    Chiba M, Masironi R (1992) Toxic and trace elements in tobacco and tobacco smoking. Bull World Health Org 70:262–269Google Scholar
  6. 6.
    E.H.C, Committee on Environmental Hazards Committee on Accident and Poison Prevention (1987) Statement on childhood lead poisoning. Pediatr 79:457–465Google Scholar
  7. 7.
    Tuthill RW (1996) Hair lead levels related to children’s classroom attention-deficit behavior. Arch Environ Health 1:214–221CrossRefGoogle Scholar
  8. 8.
    Baghurst P, Tong SL, McMichael AS, Robertson EF, Wigg NR, Vimpani GV (1992) Determinants of blood lead concentrations to age 5 years in a birth cohort study of children living in the lead smelting city of Port Pirie and surrounding areas. Arch Environ Health 47:203–210PubMedCrossRefGoogle Scholar
  9. 9.
    Goyer RA (1996) Toxic effects of metals. In: Klaassen CD (ed) Casarett and Doull’s toxicology: the basic science of poisons, vol 5. McGraw-Hill, New York, pp 691–736Google Scholar
  10. 10.
    Schramm KW (1997) Hair: a matrix for non-invasive biomonitoring of organic chemicals in mammals. Bull Environ Contam Toxicol 59:396–402PubMedCrossRefGoogle Scholar
  11. 11.
    Nowak B, Kozlowski H (1998) Heavy metals in human hair and teath: the correlation with metal concentration in the environment. Biol Trace Elem Res 62:213–228PubMedCrossRefGoogle Scholar
  12. 12.
    Morton J, Carolan VA, Gardiner PHE (2002) Removal of exogenously bound elements from human hair by various washing procedures and determination by inductively coupled plasma mass spectrometry. Anal Chim Acta 455:23–34CrossRefGoogle Scholar
  13. 13.
    Rao AN (2005) Trace element estimation—methods & clinical context. Online J Health Allied Scs 1:1Google Scholar
  14. 14.
    Torra, M, Rodamilaus, M, Cobelle J (2002) Human exposure to metal: some factors ınfluencing the metal concentrations. Trace Elem Electroly. 19(4):192–196.Google Scholar
  15. 15.
    Chatt A, Katz SA (1988) Hair analysis. VCH Publishers, New York. ISBN 0-89573-310-2Google Scholar
  16. 16.
    Kronstrand R, Förstberg-Peterson S, Kagedal B, Ahlner J, Larson G (1999) Codeine concentration in hair after oral administration is dependent on melanin content. Clin Chem 45:1485–1494PubMedGoogle Scholar
  17. 17.
    Weyermann M, Brenner H (1997) Alcohol consumption a smoking habits determinants of blood lead levels in a national population sample from Germany. Arch Environ Health 52:233–239PubMedCrossRefGoogle Scholar
  18. 18.
    Nnorom IC, Asıbanjo O, Ojı-Nnorom CG (2005) Cadmium determination in cigarettes available in Nigeria. Afr J Biotechnol 54:1128–1132Google Scholar
  19. 19.
    Watanabe T, Kasahara M, Nakatsuka H, Ikeda M (1987) Cadmium and lead contents of cigarettes in various areas of the world. Sci Total Environ 66:29–37PubMedCrossRefGoogle Scholar
  20. 20.
    Stohs SJD, Bagchi M (1997) Toxicity of trace elements in tobacco smoking. Inhal Toxicol 9:867–890CrossRefGoogle Scholar
  21. 21.
    Özden TA, Kılıc A, Vehid HE, Toparlak D, Gokcay G, Saner G (2004) Blood lead levels in school children. Indoor Built Environ 13:149–154CrossRefGoogle Scholar
  22. 22.
    Strachan DP, Cook DG (1997) Health effects of passive smoking. 1. Parental smoking and lower respiratory illness in infancy and early childhood. Thorax 52:905–914PubMedCrossRefGoogle Scholar
  23. 23.
    Lanari M, Giovannini M, Giuffre L et al (2002) Prevalence of respiratory syncytial virus infection in Italian infants hospitalized for acute lower respiratory tract infections, and association between respiratory syncytial virus infection risk factors and disease severity. Pediatr Pulmonol 33:458–465PubMedCrossRefGoogle Scholar
  24. 24.
    Afridi HI, Kazi TG, Brabazon D, Naher S (2011) Association between essential trace and toxic elements in scalp hair samples of smokers rheumatoid arthritis subjects. Sci Total Environ 15:93–100Google Scholar
  25. 25.
    Kazi TG, Jalbani N, Kazi N, Arain MB, Jamali MK, Afridi HI, Kandhro GA, Sarfraz RA, Shah AQ, Ansari R (2009) Estimation of toxic metals in scalp hair samples of chronic kidney patients. Biol Trace Elem Res 127(1):16–27PubMedCrossRefGoogle Scholar
  26. 26.
    Landsberger S, Wu D (1995) The impact of heavy metals from environmental tobacco smoke on indoor air quality as determined by Compton suppression neutron activation analysis. Sci Total Environ 173:323–337PubMedCrossRefGoogle Scholar
  27. 27.
    Chilmonczyk BA, Knight GJ, Palomaki GE, Pulkkinen AJ, Williams J, Haddow JE (1990) Environmental tobacco smoke exposure during infancy. Am J Public Health 80(10):1205–1208PubMedCrossRefGoogle Scholar
  28. 28.
    Winkelstein ML, Tarzian A, Wood RA (1997) Parental smoking behavior and passive smoke exposure in children with asthma. Ann Allergy Asthma Immunol 78(4):419–423PubMedCrossRefGoogle Scholar
  29. 29.
    Holberg CJ, Wright AL, Martinez FD, Morgan WJ, Taussig LM (1993) Child day care, smoking by caregivers, and lower respiratory tract illness in the first 3 years of life. Grp Health Med Assoc Pediatr 91(5):885–892Google Scholar
  30. 30.
    Mannino DM, Albalak R, Grosse S, Repace J (2003) Second-hand smoke exposure and blood lead levels in US children. Epidemiol 14:719–727CrossRefGoogle Scholar
  31. 31.
    Özden TA, Gökçay G, Ertem HV, Süoğlu OD, Kiliç A, Sökücü S, Saner G (2007) Elevated hair levels of cadmium and lead in school children exposed to smoking and in highways near schools. Clin Biochem 40:52–56PubMedCrossRefGoogle Scholar
  32. 32.
    Wolfsperger M, Hauser WM, Gossler W, Schlagenhaufen C (1994) Heavy metals in human hair from Austria and Italy: influence of sex and smoking habits. Sci Total Environ 156:235–242PubMedCrossRefGoogle Scholar
  33. 33.
    Willers SW, Schutz A, Attewell R, Skerfving S (1998) Relation between lead and cadmium in blood and the involuntary smoking of children. Scand J Work Environ Health 14:385–389CrossRefGoogle Scholar
  34. 34.
    Steindel S, Howanitz P (2001) The uncertainty of hair analysis for trace metals. JAMA 285:67–72CrossRefGoogle Scholar
  35. 35.
    González-Muñoz MJ, Peña A, Meseguer I (2008) Monitoring heavy metal contents in food and hair in a sample of young Spanish subjects. Food Chem Toxicol 46:3048–3052PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Muhittin A. Serdar
    • 1
    Email author
  • Beril S. Akin
    • 2
  • Cem Razi
    • 3
  • Okhan Akin
    • 4
  • Serhat Tokgoz
    • 1
  • Levent Kenar
    • 1
  • Osman Aykut
    • 5
  1. 1.Department of Clinical Biochemistry, Gülhane School of MedicineAnkaraTurkey
  2. 2.Department of Environmental SciencesGazi UniversityAnkaraTurkey
  3. 3.Department of Pediatric AllergyKecioren Training and Research HospitalAnkaraTurkey
  4. 4.Department of BiochemistryKecioren Training and Research HospitalAnkaraTurkey
  5. 5.Refik Saydam Hygiene Center PresidencyAnkaraTurkey

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