Advertisement

Biological Trace Element Research

, Volume 147, Issue 1–3, pp 285–291 | Cite as

Preventive Effects of Zinc Against Psychological Stress-Induced Iron Dyshomeostasis, Erythropoiesis Inhibition, and Oxidative Stress Status in Rats

  • Yingjie Li
  • Yuanyuan Zheng
  • Jianxin Qian
  • Xinmin Chen
  • Zhilei Shen
  • Liping Tao
  • Hongxia Li
  • Haihong Qin
  • Min LiEmail author
  • Hui ShenEmail author
Article

Abstract

Psychological stress (PS) could cause decreased iron absorption and iron redistribution in body resulting in low iron concentration in the bone marrow and inhibition of erythropoiesis. In the present study, we investigated the effect of zinc supplementation on the iron metabolism, erythropoiesis, and oxidative stress status in PS-induced rats. Thirty-two rats were divided into two groups randomly: control group and zinc supplementation group. Each group was subdivided into two subgroups: control group and PS group. Rats received zinc supplementation before PS exposure established by a communication box. We investigated the serum corticosterone (CORT) level; iron apparent absorption; iron contents in liver, spleen, cortex, hippocampus, striatum, and serum; hematological parameters; malondialdehyde (MDA); reduced glutathione (GSH); and superoxide dismutase (SOD). Compared to PS-treated rats with normal diet, the PS-treated rats with zinc supplementation showed increased iron apparent absorption, serum iron, hemoglobin, red blood cell, GSH, and SOD activities; while the serum CORT; iron contents in liver, spleen, and regional brain; and MDA decreased. These results indicated that dietary zinc supplementation had preventive effects against PS-induced iron dyshomeostasis, erythropoiesis inhibition, and oxidative stress status in rats.

Keywords

Psychological stress Zinc Iron Oxidative stress Erythropoiesis 

Notes

Acknowledgments

This work was supported in part by the grants from National Natural Science Foundation of China (no. 30872120, 81001243), Natural Science Foundation of Shanghai (no.10ZR1437400) and Shanghai Health Bureau (nos. 08GWD23, 08GWQ012).

References

  1. 1.
    Wang L, Wang WY, Zhao M, Ma L, Li M (2008) Psychological stress induces dysregulation of iron metabolism in rat brain. Neuroscience 155:24–30PubMedCrossRefGoogle Scholar
  2. 2.
    Zhao M, Chen J, Wang W, Wang L, Ma L, Shen H, Li M (2008) Psychological stress induces hypoferremia through the IL-6-hepcidin axis in rats. Biochem Biophys Res Commun 373:90–93PubMedCrossRefGoogle Scholar
  3. 3.
    Chen J, Shen H, Chen C, Wang WY, Yu SY, Zhao M, Li M (2009) The effect of psychological stress on iron absorption in rats. BMC Gastroenterol 9:83PubMedCrossRefGoogle Scholar
  4. 4.
    Wei C, Zhou J, Huang X, Li M (2008) Effects of psychological stress on serum iron and erythropoiesis. Int J Hematol 88:52–56PubMedCrossRefGoogle Scholar
  5. 5.
    Yu SY, Feng Y, Shen ZL, Li M (2011) Diet supplementation with iron augments brain oxidative stress status in a rat model of psychological stress. Nutrition 27:1048–1052PubMedCrossRefGoogle Scholar
  6. 6.
    Tuerk MJ, Fazel N (2009) Zinc deficiency. Curr Opin Gastroenterol 25:136–143PubMedCrossRefGoogle Scholar
  7. 7.
    El-Hendy HA, Yousef MI, Abo-El-Naga NI (2001) Effect of dietary zinc deficiency on hematological and biochemical parameters and concentrations of zinc, copper, and iron in growing rats. Toxicology 167:163–170PubMedCrossRefGoogle Scholar
  8. 8.
    Sreedhar B, Nair KM (2005) Modulation of aconitase, metallothionein, and oxidative stress in zinc-deficient rat intestine during zinc and iron repletion. Free Radic Biol Med 39:999–1008PubMedCrossRefGoogle Scholar
  9. 9.
    Kordas K, Stoltzfus RJ (2004) New evidence of iron and zinc interplay at the enterocyte and neural tissues. J Nutr 134:1295–1298PubMedGoogle Scholar
  10. 10.
    Song Y, Leonard SW, Traber MG, Ho E (2009) Zinc deficiency affects DNA damage, oxidative stress, antioxidant defenses, and DNA repair in rats. J Nutr 139:1626–1631PubMedCrossRefGoogle Scholar
  11. 11.
    Messaoudi I, El-Heni J, Hammouda F, Saïd K, Kerkeni A (2009) Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res 130:152–161PubMedCrossRefGoogle Scholar
  12. 12.
    Jemai H, Lachkar HA, Messaoudi I, Kerkeni A (2010) Effects of zinc pre-treatment on blood glutathione, serum zinc and kidney histological organisation in male rats exposed to cadmium. J Trace Elem Med Biol 24:277–282PubMedCrossRefGoogle Scholar
  13. 13.
    Varghese J, Faith M, Jacob M (2009) Zinc prevents indomethacin-induced renal damage in rats by ameliorating oxidative stress and mitochondrial dysfunction. Eur J Pharmacol 614:114–121PubMedCrossRefGoogle Scholar
  14. 14.
    Wales J (2009) Does psychological stress cause diabetes? Diabet Med 12:109–112CrossRefGoogle Scholar
  15. 15.
    Epel E (2009) Psychological and metabolic stress: a recipe for accelerated cellular aging? Hormones 8:7–22PubMedGoogle Scholar
  16. 16.
    WHO/UNICEF/UNU (2001) Iron deficiency anaemia: assessment, prevention, and control. World Health Organization, GenevaGoogle Scholar
  17. 17.
    WHO/UNICEF (2004) Focusing on anaemia: towards an integrated approach for effective anaemia control. World Health Organization, GenevaGoogle Scholar
  18. 18.
    Navas FJ, Cordova A (2000) Iron distribution in different tissues in rats following exercise. Biol Trace Elem Res 73:259–268PubMedCrossRefGoogle Scholar
  19. 19.
    Smith SM, Zwart SR, Block G (2005) The nutritional status of astronauts is altered after long-term space flight aboard the International Space Station. J Nutr 135:437–443PubMedGoogle Scholar
  20. 20.
    Nikolova-Todorova Z, Troic T (2003) Effect of surgical trauma on patient nutritional status. Med Arh 57:29–31PubMedGoogle Scholar
  21. 21.
    Gunshin H, Mackenzie B, Berger UV, Gunshin Y, Romero MF, Boron WF, Nussberger S, Gollan JL, Hediger MA (1997) Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388:482–488PubMedCrossRefGoogle Scholar
  22. 22.
    Yamaji S, Tennant J, Tandy S, Williams M, Singh Srai SK, Sharp P (2001) Zinc regulates the function and expression of the iron transporters DMT1 and IREG1 in human intestinal Caco-2 cells. FEBS Lett 507:137–141PubMedCrossRefGoogle Scholar
  23. 23.
    Kraus A, Roth H, Kirchgessner M (1997) Supplementation with vitamin C, vitamin E or β-carotene influences osmotic fragility and oxidative damage of erythrocytes of zinc-deficient rats. J Nutr 127:1290–1296PubMedGoogle Scholar
  24. 24.
    Liew CW, Rand KD, Simpson RJ, Yung WW, Mansfield RE, Crossley M, Proetorius-Ibba M, Nerlov C, Poulsen FM, Mackay JP (2006) Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1. J Biol Chem 281:28296–28306PubMedCrossRefGoogle Scholar
  25. 25.
    King LE, Frentzel JW, Mann JJ, Fraker PJ (2005) Chronic zinc deficiency in mice disrupted T cell lymphopoiesis and erythropoiesis while B cell lymphopoiesis and myelopoiesis were maintained. J Am Coll Nutr 24:494–502PubMedGoogle Scholar
  26. 26.
    Leberbauer C, Boulmé F, Unfried G, Huber J, Beug H, Müllner EW (2005) Different steroids co-regulate long-term expansion versus terminal differentiation in primary human erythroid progenitors. Blood 105:85–94PubMedCrossRefGoogle Scholar
  27. 27.
    Takeda A, Tamano H (2009) Insight into zinc signaling from dietary zinc deficiency. Brain Res Rev 62:33–34PubMedCrossRefGoogle Scholar
  28. 28.
    Kelly G (1999) Nutritional and botanical interventions to assist with the adaptation to stress. Altern Med Rev 4:249PubMedGoogle Scholar
  29. 29.
    Levenson CW, Tassabehji NM (2004) Iron and ageing: an introduction to iron regulatory mechanisms. Ageing Res Rev 3:251–263PubMedCrossRefGoogle Scholar
  30. 30.
    Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1–40PubMedCrossRefGoogle Scholar
  31. 31.
    Biswas S, Rahman I (2009) Environmental toxicity, redox signaling and lung inflammation: the role of glutathione. Mol Aspects Med 30:60–76PubMedCrossRefGoogle Scholar
  32. 32.
    Fleming D, Tucker K, Jacques P, Dallal G, Wilson P, Wood R (2002) Dietary factors associated with the risk of high iron stores in the elderly Framingham Heart Study cohort. Am J Clin Nutr 76:1375–1384PubMedGoogle Scholar
  33. 33.
    Milman N, Byg K, Ovesen L, Kirchhoff M, Jurgensen K (2002) Iron status in Danish men 1984–94: a cohort comparison of changes in iron stores and the prevalence of iron deficiency and iron overload. Eur J Haematol 68:332–340PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Yingjie Li
    • 1
  • Yuanyuan Zheng
    • 2
  • Jianxin Qian
    • 3
  • Xinmin Chen
    • 1
  • Zhilei Shen
    • 1
  • Liping Tao
    • 1
  • Hongxia Li
    • 1
  • Haihong Qin
    • 1
  • Min Li
    • 1
    Email author
  • Hui Shen
    • 1
    Email author
  1. 1.Department of Military HygieneSecond Military Medical UniversityShanghaiChina
  2. 2.Department of ImmunologySecond Military Medical UniversityShanghaiChina
  3. 3.Department of Medical OncologyChangzheng HospitalShanghaiChina

Personalised recommendations