Advertisement

Biological Trace Element Research

, Volume 147, Issue 1–3, pp 320–328 | Cite as

The Influence of Selenium on Root Growth and Oxidative Stress Induced by Lead in Vicia faba L. minor Plants

  • Magdalena Mroczek-Zdyrska
  • Małgorzata Wójcik
Article

Abstract

The effect of selenium (Se) on Vicia faba L. minor roots subjected to lead (Pb) stress was studied by investigating root growth, root viability, and antioxidant enzyme activity. The experiments were carried out on plants grown for 2 weeks on Hoagland medium supplied with 50 μM Pb in the form of lead nitrate Pb(NO3)2 and/or Se concentrations of 1.5 and 6 μM in the form of sodium selenite Na2SeO3. It was shown that Pb reduced the root growth and caused serious damage in the roots, which was accompanied by metal accumulation in these tissues. The exposition of roots to Pb led to significant changes in the biochemical parameters: the MDA and T-SH content and glutathione peroxidase (GSH-Px) activity increased but the guaiacol peroxidase (GPOX) activity decreased. Moreover, Pb intensified O 2 ●− production in the roots. Selenium at a lower concentration alleviated Pb toxicity which was accompanied by a decreased O 2 ●− production in the apical parts of roots and increased the T-SH content and GPOX activity. However, higher Se concentration intensified MDA and T-SH accumulation and GPOX and GSH-Px activity in Pb-treated plant roots. At low concentration, Se improved cell viability whereas at high concentration it was pro-oxidant and enhanced the lipid peroxidation and cell membrane injury.

Keywords

Antioxidant enzymes Lead Oxidative stress Selenium Vicia faba L. 

Abbreviations

CAT

Catalase

DHE

Dihydroethidium

DTNB

5,5′-Dithiobis(2-nitrobenzoic acid)

FDA

Fluorescein diacetate

GPOX

Guaiacol peroxidase

GR

Glutathione reductase

GSH

Reduced glutathione

GSH-Px

Glutathione peroxidase

MDA

Malondialdehyde

PI

Propidium iodide

SSA

Sulfosalicylic acid

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

References

  1. 1.
    Needleman H (2004) Lead poisoning. Annu Rev Med 55:209–222PubMedCrossRefGoogle Scholar
  2. 2.
    Sharma P, Dubey RS (2005) Lead toxicity in plants. Braz J Plant Physiol 17:35–52CrossRefGoogle Scholar
  3. 3.
    Sengar RS, Gautam M, Sengar RS, Garg SK, Sengar K, Chaudhary R (2008) Lead stress effects on physiobiochemical activities of higher plants. Rev Environ Contam Toxicol 196:73–93PubMedCrossRefGoogle Scholar
  4. 4.
    Liu D, Jiang W, Liu C, Xin C, Hou W (2000) Uptake and accumulation of lead by roots, hypocotyls and shoots of Indian mustard Brassica juncea L. Bioresource Technol 71:273–277CrossRefGoogle Scholar
  5. 5.
    Cerklewski FL, Forbes RM (1976) Influence of dietary selenium on lead toxicity in the rat. J Nutr 106:778–783PubMedGoogle Scholar
  6. 6.
    Soudani N, Sefi M, Ben Amara I, Boudawara T, Zeghal N (2010) Protective effects of selenium (Se) on chromium (VI) induced nephrotoxicity in adult rats. Ecotox Environ Safe 73:671–678CrossRefGoogle Scholar
  7. 7.
    Ikemoto T, Kunito T, Tanaka H, Baba N, Miyazaki N, Tanabe S (2004) Detoxification mechanism of heavy metals in marine mammals and seabirds: interaction of selenium with mercury, silver, copper, zinc and cadmium in liver. Arch Environ Con Tox 47:402–413CrossRefGoogle Scholar
  8. 8.
    He PP, Lv XZ, Wang GY (2004) Effects of Se and Zn supplementation on the antagonism against Pb and Cd in vegetables. Environ Int 30:167–172PubMedCrossRefGoogle Scholar
  9. 9.
    Fargašová A, Pastierová J, Svetková K (2006) Effect of Se–metal pair combinations (Cd, Zn, Cu, Pb) on photosynthetic pigments production and metal accumulation in Sinapis alba L. seedlings. Plant Soil Environ 52:8–15Google Scholar
  10. 10.
    Pedrero Z, Madrid Y, Hartikainen H, Cámara C (2008) Protective effect of selenium in broccoli (Brassica oleracea) plants subjected to cadmium exposure. J Agric Food Chem 54:2412–2417CrossRefGoogle Scholar
  11. 11.
    Feng R, Wei C, Tu S, Sun X (2009) Interactive effects of selenium and arsenic on their uptake by Pteris vittata L. under hydroponic conditions. Environ Exp Bot 65:363–368CrossRefGoogle Scholar
  12. 12.
    Filek M, Keskinen R, Hartikainen H, Szarejko I, Janiak A, Miszalski Z, Golda A (2008) The protective role of selenium in rape seedlings subjected to cadmium stress. J Plant Physiol 165:833–844PubMedCrossRefGoogle Scholar
  13. 13.
    Terry N, Zayed M, de Souza MP, Tarun AS (2000) Selenium in higher plants. Annu Rev Plant Physiol Plant Mol Biol 51:401–432PubMedCrossRefGoogle Scholar
  14. 14.
    Ellis DR, Salt DE (2003) Plants, selenium and human health. Curr Opin Plant Biol 6:273–279PubMedCrossRefGoogle Scholar
  15. 15.
    Sors TG, Ellis DR, Salt DE (2005) Selenium uptake, translocation, assimilation and metabolic fate in plants. Photosynth Res 86:373–389PubMedCrossRefGoogle Scholar
  16. 16.
    Hartikainen H, Xue T, Piironen V (2000) Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant Soil 225:193–200CrossRefGoogle Scholar
  17. 17.
    Xue T, Hartikainen H, Piironen V (2001) Antioxidative and growth-promoting effect of selenium in senescing lettuce. Plant Soil 27:55–61CrossRefGoogle Scholar
  18. 18.
    Cartes P, Gianfera L, Mora ML (2005) Uptake of selenium and its antioxidative activity in ryegrass when applied a selenate and selenite forms. Plant Soil 276:359–367CrossRefGoogle Scholar
  19. 19.
    Djanaguiraman M, Devi DD, Shanker AK, Sheeba A, Bangarusamy U (2005) Selenium—an antioxidative protectant in soybean during senescence. Plant Soil 272:77–86CrossRefGoogle Scholar
  20. 20.
    Kong L, Wang M, Bi D (2005) Selenium modulates the activities of antioxidant enzymes, osmotic homeostasis and promotes the growth of sorrel seedlings under salt stress. Plant Growth Regul 45:155–163CrossRefGoogle Scholar
  21. 21.
    Hasanuzzaman M, Hossain MA, Fujita M (2010) Selenium in higher plants: physiological role, antioxidant metabolism and abiotic stress tolerance. J Plant Sci 5:354–375CrossRefGoogle Scholar
  22. 22.
    Kápolna E, Hillestrøm PR, Laursen KH, Husted S, Larsen EH (2009) Effect of foliar application of selenium on its uptake and speciation in carrot. Food Chem 115:1357–1363CrossRefGoogle Scholar
  23. 23.
    Glińska S, Gabara B (2002) Influence of selenium on lead absorption and localization in meristematic cells of Allium sativum L. and Pisum sativum L. roots. Acta Biol Cracov Bot 44:39–48Google Scholar
  24. 24.
    Vorobets N (2006) Glutathione peroxidase activity in sunflower shoots exposed to lead and selenium. Annales UMCS Section DDD, Vol. XIX, 1: 151–153Google Scholar
  25. 25.
    Schützendübel A, Polle A (2002) Plant responses to abiotic stressess: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1365PubMedCrossRefGoogle Scholar
  26. 26.
    Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci 151:59–66CrossRefGoogle Scholar
  27. 27.
    Hoagland DR, Arnon DJ (1959) The water-culture method of growing plants without soil. Calif Agr Expt Sta Circ 347:26–29Google Scholar
  28. 28.
    Wierzbicka M, Potocka A (2002) Lead tolerance in plants growing on dry and moist soil. Acta Biol Cracov Bot 44:21–28Google Scholar
  29. 29.
    Pan JW, Zhu MY, Chen H (2001) Aluminium-induced cell death in root-tip cells of barley. Environ Exp Bot 46:71–79PubMedCrossRefGoogle Scholar
  30. 30.
    Tamás L, Budíková S, Šimonovičová M, Huttová J, Široká B, Mistrík I (2006) Rapid and simple method for Al-toxicity analysis in emerging barley roots during germination. Biol Plant 50:87–93CrossRefGoogle Scholar
  31. 31.
    Yamamoto Y, Kobayashi Y, Devi SR, Rikiishi S, Matsumoto H (2002) Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells. Plant Physiol 128:63–72PubMedCrossRefGoogle Scholar
  32. 32.
    Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198PubMedCrossRefGoogle Scholar
  33. 33.
    Maas FM, de Kok LJ, Peters JL, Kuiper PJC (1987) A comparative study on the effects of H2S and SO2 fumigation on the growth and accumulation of sulfate and sulfhydryl compounds in Trifolium pretense L., Glycine max Merr. and Phaseolus vulgaris L. J Exp Bot 38:1459–1469CrossRefGoogle Scholar
  34. 34.
    Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126PubMedCrossRefGoogle Scholar
  35. 35.
    Ali MB, Hahn EJ, Paek KY (2006) Copper-induced changes in the growth, oxidative metabolism and saponin production in suspension culture roots of Panax ginseng in bioreactors. Plant Cell Rep 25:1122–1132PubMedCrossRefGoogle Scholar
  36. 36.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  37. 37.
    Grejtovský A, Markušová K, Nováková L (2008) Lead uptake by Matricaria chamomilla L. Plant Soil Environ 54:47–54Google Scholar
  38. 38.
    Kuznetsov Vas V, Kholodova VP, VlV K, Yagodin BA (2003) Selenium regulates the water status of plants exposed to drought. Dokl Biol Sci 390:266–268PubMedCrossRefGoogle Scholar
  39. 39.
    Quartacci MF, Cosi E, Navari-Izzo F (2001) Lipids and NADPH-dependent superoxide production in plasma membrane vesicles from roots of wheat grown under copper deficiency or excess. J Exp Bot 52:77–84PubMedCrossRefGoogle Scholar
  40. 40.
    Hejnowicz Z (2002) Anatomia i histogeneza roślin naczyniowych. Organy wegetatywne. Wydawnictwo Naukowe PWN, Warszawa (in Polish)Google Scholar
  41. 41.
    de la Luz MM, Pinilla L, Rosas A, Cartes P (2008) Selenium uptake and its influence on the antioxidative system of white clover as affected by lime and phosphorus fertilization. Plant Soil 303:139–149CrossRefGoogle Scholar
  42. 42.
    Nowak J, Kaklewski K, Ligocki M (2004) Influence of selenium on oxidoreductive enzymes activity in soil and in plants. Soil Biol Biochem 36:1553–1558CrossRefGoogle Scholar
  43. 43.
    Shanker AK, Djanaguiraman M, Sudhagar R, Chandrashekar CN, Pathmanabhan G (2004) Differential antioxidative response of ascorbate glutathione pathway enzymes and metabolites to chromium speciation stress in reengram (Vigna radiata (L.) R. Wilczek. cv CO 4) roots. Plant Sci 166:1035–1043CrossRefGoogle Scholar
  44. 44.
    Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832PubMedCrossRefGoogle Scholar
  45. 45.
    Maitani T, Kubota H, Sato K, Hamada T (1996) The composition of metal bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol 110:1145–1150PubMedGoogle Scholar
  46. 46.
    Hawrylak B, Szymańska M (2004) Selenium as a sulphydrylic group inductor in plants. Cell Mol Biol Lett 9:329–336PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Magdalena Mroczek-Zdyrska
    • 1
  • Małgorzata Wójcik
    • 2
  1. 1.Department of Cell BiologyMaria Curie-Skłodowska UniversityLublinPoland
  2. 2.Department of Plant PhysiologyMaria Curie-Skłodowska UniversityLublinPoland

Personalised recommendations