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Biological Trace Element Research

, Volume 147, Issue 1–3, pp 180–188 | Cite as

Modified Natural Clinoptilolite Detoxifies Small Mammal’s Organism Loaded with Lead I. Lead Disposition and Kinetic Model for Lead Bioaccumulation

  • Michaela Beltcheva
  • Roumiana Metcheva
  • Nikolay Popov
  • Svetla E. Teodorova
  • J. Antonio Heredia-Rojas
  • Abraham O. Rodríguez-de la Fuente
  • Laura E. Rodríguez-Flores
  • Margarita Topashka-Ancheva
Article

Abstract

Zeolites, especially clinoptilolites, have wide application in removing heavy metals from different solutions and wastewater. The detoxification capacity of the clinoptilolite sorbent KLS–10-MA, a modified natural Bulgarian zeolite, applied as a food supplement in conditions of an ecotoxicological experiment with conventional food and lead was demonstrated for the first time. Laboratory mice, inbred imprinting control region strain, were used in a 90-day ecotoxicological experiment. Animals were divided into four experimental groups. Lead bioaccumulations in exposed and non-supplemented/supplemented with KLS–10-MA animals were compared. As additional control, healthy animals non-exposed to Pb were fed with conventional forage mixed with 12.5% KLS–10-MA. The dietary inclusion of the sorbent reduced Pb concentrations in exposed and supplemented mice by 84%, 89%, 91%, 77%, and 88% in carcass, liver, kidneys, bones, and feces, respectively. A mathematical model was proposed to outline the common trends of bone Pb bioaccumulation in exposed and non-supplemented/supplemented animals. Characteristic parameters of the kinetics of Pb concentrations were determined. Based on the model, the coefficient of absorption of Pb by gastrointestinal mucosa in the supplemented mice was found—η = 3.53% (versus η = 15% in non-supplemented ones). The present study clearly indicates that there is a realistic perspective to create a new drug based on modified natural clinoptilolites in cases of chronic heavy metal intoxication, without negatively affecting the environment.

Keywords

Lead bioaccumulation Clinoptilolite Detoxification Laboratory mice Mathematical model Carcass Liver Kidneys Bone Feces 

Notes

Acknowledgments

Authors express their special thanks to “Mineral agro Z” LTD–Bulgaria for the total financial support of this work.

References

  1. 1.
    Goyer RA (1996) Toxic effects of metals. In: McGraw-Hill (ed) Casarett and Doull’s toxicology. The basic science of poisons, fifth edition. Health Professions Revision, New York • London • Tokyo • Toronto, pp 691–735Google Scholar
  2. 2.
    O’Flaherty EJ (1998) Physiologically based models of metal kinetics. Crit Rev Toxicol 28:271–317PubMedCrossRefGoogle Scholar
  3. 3.
    Robles HV, Romo E, Sanchez-Mendoza A, Rios A, Soto V, Avila-Casado MC, Medina A, Escalante B (2007) Lead exposure effect on angiotensin II renal vasoconstriction. Hum Exp Toxicol 26:499–507PubMedCrossRefGoogle Scholar
  4. 4.
    Johnson FM (1998) The genetic effects of environmental lead. Mutat Res-Rev Mutat 410:123–140CrossRefGoogle Scholar
  5. 5.
    Topashka-Ancheva M, Metcheva R, Teodorova SE (2003) Bioaccumulation and damaging action of polymetal industrial dust on laboratory mice Mus musculus alba II. Genetic, cell, and metabolic disturbances. Environ Res 92:152–160PubMedCrossRefGoogle Scholar
  6. 6.
    Valverde M, Trejo C, Rojas E (2005) Is the capacity of lead acetate and cadmium chloride to induce genotoxic damage due to direct DNA-metal interaction? Toxicol Ind Health 21:243–248CrossRefGoogle Scholar
  7. 7.
    Tao YF, Qui Y, Fang SY, Liu ZY, Wang Y, Zhu JH (2010) Trapping the lead ion in multi-component aqueous solutions by natural clinoptilolite. J Hazard Mater 180:282–288PubMedCrossRefGoogle Scholar
  8. 8.
    Papaioannou D, Katsoulos PD, Panousis N, Karatzias H (2005) The role of natural and synthetic zeolites as feed additives on the prevention and/or the treatment of certain farm animal diseases: a review. Micropor Mesopor Mat 84:161–170CrossRefGoogle Scholar
  9. 9.
    Stylianou MA, Hadjiconstantinou MP, Inglezakis VJ, Moustakas KG, Loizidou MD (2007) Use of natural clinoptilolite for the removal of lead, copper and zinc in fixed bed column. J Hazard Mater 143:575–581PubMedCrossRefGoogle Scholar
  10. 10.
    Sheppard RA (1984) Characterization of zeolitic materials in agricultural research. In: Pond WG, Mumption FA (eds) Zeo-agriculture: use of natural zeolites in agriculture and aquaculture. Westview, Boulder, Colorado, pp 81–90Google Scholar
  11. 11.
    Bernal MP, Lopez-Real JM, Scott KM (1993) Application of natural zeolites for the reduction of ammonia emissions during the composting of organic wastes in a laboratory composting simulator. Bioresource Technol 43:35–39CrossRefGoogle Scholar
  12. 12.
    Mumpton FA (1960) Clinoptilolite redefined. Am Mineral 45:351–369Google Scholar
  13. 13.
    Elaiopoulos K, Perraki T, Grigoropoulou E (2008) Mineralogical study and porosimetry measurements of zeolites from Scaloma area, Thrace, Greece. Micropor Mesopor Mat 112:441–449CrossRefGoogle Scholar
  14. 14.
    Godelitsas A, Armbruster T (2003) HEU-zeolites modified by transition elements and lead. Micropor Mesopor Mat 61:3–24CrossRefGoogle Scholar
  15. 15.
    Mumpton FA (1999) La Roca Magica: uses of natural zeolites in agriculture and industry. Proc Nat Acad Sci U S A 96:3463–3470CrossRefGoogle Scholar
  16. 16.
    Wingenfelder U, Nowack B, Furrer G, Schulin R (2005) Adsorption of Pb and Cd by amine-modified zeolite. Water Res 39:3287–3297PubMedCrossRefGoogle Scholar
  17. 17.
    Inglezakis VJ, Stylianou MA, Gkantzou D, Loizidou MD (2007) Removal of Pb(II) from aqueous solutions by using clinoptilolite and bentonite as adsorbents. Desalination 210:248–256CrossRefGoogle Scholar
  18. 18.
    Sprynskyy P, Kosobucki M, Kowalkowski T, Buszewski B (2007) Influence of clinoptilolite rock on chemical speciation of selected heavy metals in sewage sludge. J Hazard Mater 149:310–316PubMedCrossRefGoogle Scholar
  19. 19.
    Llanes-Monter MM, Olguin MT, Solache-Rios MJ (2007) Lead sorption by a Mexican, clinoptilolite-rich tuff. Environ Sci Pollut Res Int 14:397–403PubMedCrossRefGoogle Scholar
  20. 20.
    Argun ME (2008) Use of clinoptilolite for the removal of nickel ions from water: kinetics and thermodynamics. J Hazard Mater 150:587–595PubMedCrossRefGoogle Scholar
  21. 21.
    Orhan Y, Kocaoba S (2007) Adsorption of toxic metals by natural and modified clinoptilolite. Ann Chim 97:781–790PubMedCrossRefGoogle Scholar
  22. 22.
    Pitcher SK, Slade RCT, Ward NI (2004) Heavy metal removal from motorway stormwater using zeolites. Sci Total Environ 334–335:161–166PubMedGoogle Scholar
  23. 23.
    Kazemian H, Mallah MH (2006) Elimination of Cd2+ and Mn2+ from wastewaters using natural clinoptilolite and synthetic zeolite P. Iran J Chem Chem Eng 25:91–94Google Scholar
  24. 24.
    Oter O, Akcay H (2007) Use of natural clinoptilolite to improve water quality: sorption and selectivity studies of lead(II), copper(II), zinc(II), and nickel(II). Water Environ Res 79:329–335PubMedCrossRefGoogle Scholar
  25. 25.
    Popov N, Jilov G, Popova T (1997) Study of the use of natural clinoptilolites and their modifications as effective sorbents of Sr and Cs and heavy metals from water solutions and drinking waters. 5-th International Conference of Natural Zeolites “Zeolite–97”, September 21–29, Ischia (Naples), ItalyGoogle Scholar
  26. 26.
    Kosobucki P, Kruk M, Buszewski B (2008) Immobilization of selected heavy metals in sewage sludge by natural zeolites. Bioresource Technol 99:5972–5976CrossRefGoogle Scholar
  27. 27.
    Cammack KM, Wright CL, Austin KJ, Johnson PS, Cockrum RR, Kessler KL, Olson KC (2010) Effects of high-sulfur water and clinoptilolite on health and growth performance of steers fed forage-based diets. J Anim Sci 88:1777–1785PubMedCrossRefGoogle Scholar
  28. 28.
    Dwairi IM (1998) Conserving toxic ammoniacal nitrogen in manure using natural zeolite tuff: a comparative study. Bull Environ Contam Toxicol 60:126–133PubMedCrossRefGoogle Scholar
  29. 29.
    Mumpton FA (1983) The role of natural zeolites in agriculture and aquaculture. In: Pond WG, Mumption FA (eds) Zeo-agriculture: use of natural zeolites in agriculture and aquaculture. Westview, Boulder, Colorado, pp 33–43Google Scholar
  30. 30.
    EMFEMA (2005) International association of the European manufacturers of major, trace and specific feed minerals secretariat. Wetstraat-Rue de la Loi 223/3B-1040 Brussels, Belgium. Available at http://www.emfema.org/minerals
  31. 31.
    Topashka-Ancheva M, Beltcheva M, Metcheva R, Heredia Rojas JA, Rodriguez De la Fuente AO, Gerasimova Ts, Rodríguez-Flores LE, Teodorova SE (2011) Modified natural clinoptilolite detoxifies small mammal’s organism loaded with lead II. Genetic, cell, and physiological effects. Biol Trace Elem Res (in press)Google Scholar
  32. 32.
    Ming DW, Dixon JB (1987) Quantitative determination of clinoptilolite in soils by a cation-exchange capacity method. Clays Clay Miner 35:463–468CrossRefGoogle Scholar
  33. 33.
    Sawicka-Kapusta K, Gorecki A, Swiergosz R, Juszczak G, Mielczarek M, Wojcik B (1987) Effect of metabolic rate on the rate of elimination of high and low concentrations of cadmium and lead in the bank vole. Ecologia Polska 35:399–430Google Scholar
  34. 34.
    Ershov YA, Pleteneva TV (1989) Mechanisms of toxic action of inorganic compounds. Medizina Publ, House, Moscow (in Russian)Google Scholar
  35. 35.
    Alexandrov L (1971) Regularized computerizing processes of Newton-Kantarovich type. JVM i MF 2:31–41 (In Russian)Google Scholar
  36. 36.
    Alexandrov L, Drenska M, Karadjov D (1984) Program System REGN for solution of nonlinear system of equations PRS-165/REGN RSIC. Ooak Ridge, TenneseeGoogle Scholar
  37. 37.
    Ivanova T, Stoyanov I, Stoilov G, Kostov P, Sapunova S (1997) In: Kirov G, Filizova L, Petrov O (eds) Natural zeolites—Sofia ’95. Pensoft, SofiaGoogle Scholar
  38. 38.
    Rajnov N, Popov N, Janev J, Popova T, Parvanov B, Konkin G (1997) Mineralogical–physico–chemical and technological characteristics of zeolitesed tuff deposits in the Eastern Rhodopes. Natural Zeolites 2:263–275Google Scholar
  39. 39.
    Rodriguez-Fuentes G, Barrinos M, Iraizoz A, Perdomo I, Cedre B (1997) Enterex: anti-diarrheic drug based on purified natural clinoptilolite. Zeolites 19:441–448CrossRefGoogle Scholar
  40. 40.
    Tiwari J (2007) Zeolite as natural feed additives to reduce environmental impacts of swine manure. Department of Bioresource Engineering, McGill University, MontrealGoogle Scholar
  41. 41.
    Zhou CF, Zhu JH (2005) Adsorption of nitrosamines in acidic solution by zeolites. Chemosphere 58:109–114PubMedCrossRefGoogle Scholar
  42. 42.
    Sprynskyy M, Buszewski B, Terzyk AP, Namielsnik J (2006) Study of the selection mechanism of heavy metal (Pb2+, Cu2+, Ni2+, and Cd2+) adsorption on clinoptilolite. J Colloid Interf Sci 304:21–28CrossRefGoogle Scholar
  43. 43.
    Djurova E, Stefanova I, Gradev G (1989) Geological, mineralogical, and ion exchange characteristics of zeolite rocks from Bulgaria. J Radioanal Nucl Ch, Articles 130:425–432CrossRefGoogle Scholar
  44. 44.
    Çulfaz M, Yagiz M (2004) Ion exchange properties of natural clinoptilolite: lead–sodium and cadmium–sodium equilibria. Sep Purif Technol 37:93–105CrossRefGoogle Scholar
  45. 45.
    Moattar F, Hayeripour S (2004) Application of chitin and zeolite adsorbents for treatment of low level radioactive liquid wastes. Int J Environ Sci Technol 1:45–50Google Scholar
  46. 46.
    Samuels ER, Meranger JC, Tracy BL, Subramanian KS (1989) Lead concentrations in human bones from the Canadian population. Sci Total Environ 89:261–269PubMedCrossRefGoogle Scholar
  47. 47.
    Hamilton JD, O’Flaherty EJ (1995) Influence of lead on the mineralization during bone growth. Fund Appl Toxicol 26:265–271CrossRefGoogle Scholar
  48. 48.
    O’Flaherty EJ (2000) Modeling normal aging bone loss, with consideration of bone loss in osteoporosis. Toxicol Sci 55:171–188PubMedCrossRefGoogle Scholar
  49. 49.
    Pond WG (1996) Bone density and tissue lead accretion in growing rats fed low high calcium with or without supplemental clinoptilolite. Bull Environ Contam Toxicol 57:713–721PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Michaela Beltcheva
    • 1
  • Roumiana Metcheva
    • 1
  • Nikolay Popov
    • 2
  • Svetla E. Teodorova
    • 3
  • J. Antonio Heredia-Rojas
    • 4
  • Abraham O. Rodríguez-de la Fuente
    • 4
  • Laura E. Rodríguez-Flores
    • 5
  • Margarita Topashka-Ancheva
    • 1
  1. 1.Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of SciencesSofiaBulgaria
  2. 2.Mineralagro LTDSofiaBulgaria
  3. 3.Institute for Nuclear Research and Nuclear Energy, Bulgarian Academy of SciencesSofiaBulgaria
  4. 4.Departamento de Ciencias Exactas y Desarrollo Humano, Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónSan Nicolás de los GarzaMexico
  5. 5.Departamento de Patología, Facultad de MedicinaUniversidad Autónoma de Nuevo LeónMonterreyMexico

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