Biological Trace Element Research

, Volume 146, Issue 3, pp 335–339 | Cite as

Influence of Methionine and Vitamin E on Fluoride Concentration in Bones and Teeth of Rats Exposed to Sodium Fluoride in Drinking Water

  • Iwona Błaszczyk
  • Ewa Birkner
  • Izabela Gutowska
  • Ewa Romuk
  • Dariusz Chlubek
Article

Abstract

Increased exposure to fluorine-containing compounds leads to accumulation of fluorides in hard tissues of bones and teeth, which may result in numerous skeletal and dental disorders. This study evaluates the influence of methionine and vitamin E on fluoride concentration in bones and teeth of rats subjected to long-term exposure to sodium fluoride in drinking water. The study was conducted in 30 3-month-old female Wistar FL rats. The animals were divided into five groups, six rats per group. The control group consisted of rats receiving only distilled water as drinking water. All other groups received NaF in the amount of 10 mg/kg of body mass/day in their drinking water. In addition, respective animal groups received: NaF + Met group—10 mg of methionine/kg of body mass/day, NaF + Met + E group—10 mg of methionine/kg of body mass/day and 3 mg of vitamin E (tocopheroli acetas)/rat/day and NaF + E group—3 mg of vitamin E/rat/day. Femoral bones and incisor teeth were collected for the study, and the fluoride concentration was determined using a fluoride ion-selective electrode. Fluoride concentration in both bones and teeth was found to be higher in the NaF and NaF + Met groups compared to the control group. In groups NaF + Met + E and NaF + E, the study material contained much lower fluoride concentration compared to the NaF group, while the effect was more prominent in the NaF + E group. The results of the studies indicate that methionine and vitamin E have opposite effects on accumulation of fluorides in hard tissue in rats. By stimulating fluoride accumulation, methionine reduces the adverse effect of fluorides on soft tissue, while vitamin E, which prevents excessive accumulation of fluorides in bones and teeth, protects these tissues from fluorosis. Therefore, it seems that combined application of both compounds would be optimal for the prevention of the adverse effects of chronic fluoride intoxication.

Keywords

Bones Teeth Sodium fluoride (NaF) Methionine (Met) Vitamin E Rats 

References

  1. 1.
    Inkielewicz I, Krechniak J (2003) Fluoride content in soft tissues and urine of rats exposed to sodium fluoride in drinking water. Fluoride 36:263–266Google Scholar
  2. 2.
    Ekstrand J, Ziegler EE, Nelson SE, Forman SJ (1994) Absorption and retention of dietary and supplemental fluoride by infants. Adv Dent Res 8:175–180PubMedGoogle Scholar
  3. 3.
    Błaszczyk I, Birkner E, Kasperczyk S (2011) Influence of methionine on toxicity of fluoride in the liver of rats. Biol Trace Elem Res 139:325–331PubMedCrossRefGoogle Scholar
  4. 4.
    Guo X, Sun G, Sun Y (2003) Oxidative stress from fluoride-induced hepatotoxicity in rats. Fluoride 36:25–29Google Scholar
  5. 5.
    Błaszczyk I, Grucka-Mamczar E, Kasperczyk S, Birkner E (2010) Influence of methionine upon the activity of antioxidative enzymes in the kidney of rats exposed to sodium fluoride. Biol Trace Elem Res 133:60–70PubMedCrossRefGoogle Scholar
  6. 6.
    Shashi A, Singh JP, Thapar SP (2002) Toxic effects of fluoride on rabbit kidney. Fluoride 35:38–50Google Scholar
  7. 7.
    Shivarajashankara YM, Shivashankara AR, Gopalakrishna BP, Hanumanth RS (2002) Brain lipid peroxidation and antioxidant systems of young rats in chronic fluoride intoxication. Fluoride 35:197–202Google Scholar
  8. 8.
    Chen X, Machida K, Isizaki K, Nisio N, Ando M (2000) Pulmonary effects of sodium fluoride aerosol on rats. Fluoride 33:159–167Google Scholar
  9. 9.
    Dąbkowska E, Chlubek D, Machoy-Mokrzyńska A, Machoy Z, Ogoński T, Raczyński J, Gębczyńska Z (1995) Fluorine content in the elk (Alces alces L.) teeth. Bromat ChemToksykol 28:129–132Google Scholar
  10. 10.
    Dąbkowska E, Chlubek D, Machoy-Mokrzyńska A, Machoy Z, Ogoński T, Raczyński J, Gębczyńska Z (1995) Fluorine accumulation in the elk (Alces alces L.) mandibles vs individuals' age. Bromat Chem Toksykol 28:123–127Google Scholar
  11. 11.
    Grucka-Mamczar E, Machoy Z, Tarnawski R, Birkner E, Polaniak R, Mamczar A (1998) Influence of fluoride in drinking water on levels of fluoride, magnesium and calcium in rat teeth. Czas Stomatol 51:427–432Google Scholar
  12. 12.
    Grucka-Mamczar E, Chlubek D, Birkner E, Zalejska-Fiolka J, Błaszczyk I, Kasperczyk S, Stawiarska-Pięta B (2006) The effect of caffeine and sodium fluoride on fluoride concentration in serum and its content in teeth and bones of rats. Ann Acad Med Stetin 52:37–40PubMedGoogle Scholar
  13. 13.
    Wang J, Guo Y, Liang Z, Hao J (2003) Amino acid composition and histopathology of goat teeth in an industrial fluoride polluted area. Fluoride 36:177–184Google Scholar
  14. 14.
    Li DS, Curtess TW, Pearce EIF, Coote GE (1996) Effects of arsenic or/and fluoride on mineralized tissues of the rat. Fluoride 29:156–162Google Scholar
  15. 15.
    Błaszczyk I, Birkner E, Romuk E (2009) The effect of methionine on toxicity of fluoride in blood plasma of rats. Farm Przegl Nauk 12:11–14Google Scholar
  16. 16.
    Błaszczyk I, Grucka-Mamczar E, Kasperczyk S, Birkner E (2009) Influence of methionine upon the concentration of malondialdehyde in the tissue and blood of rats exposed to sodium fluoride. Biol Trace Elem Res 129:229–238PubMedCrossRefGoogle Scholar
  17. 17.
    Grucka-Mamczar E, Birkner E, Blaszczyk I, Kasperczyk S, Wielkoszyński T, Świętochowska E, Stawiarska-Pięta B (2009) The influence of sodium fluoride and antioxidants on the concentration of malondialdehyde in rat blood plasma. Fluoride 42:101–104Google Scholar
  18. 18.
    Błaszczyk I, Grucka-Mamczar E, Kasperczyk S, Birkner E (2008) Influence of fluoride on rat kidney antioxidant system: effects of methionine and vitamin E. Biol Trace Elem Res 121:51–59PubMedCrossRefGoogle Scholar
  19. 19.
    Yamaguchi M (2007) Fluoride and bone metabolism. Clin Calcium 17:217–223PubMedGoogle Scholar
  20. 20.
    Ma J, Li M, Song Y, Tu J, Liu F, Liu K (2009) Serum osteocalcin and calcitonin in adult males with different fluoride exposures. Fluoride 42:133–136Google Scholar
  21. 21.
    Machoy Z (1987) Biochemical mechanisms of the effect of fluorine compounds. Folia Med Cracov 28:61–81PubMedGoogle Scholar
  22. 22.
    Tamer MN, Kale Koroglu B, Arslan C, Akdogan M, Kóroglu M, Cam H, Yildiz M (2007) Osteosclerosis due to endemic fluorosis. Sci Total Environ 373:43–48PubMedCrossRefGoogle Scholar
  23. 23.
    Grucka-Mamczar E, Birkner E, Chlubek D, Samujło D, Kasperczyk S, Kasperczyk A (2004) Concentration of fluoride ions in serum, teeth and bones of rats with hyperglycemia induced by high doses of sodium fluoride. Bromat Chem Toksykol 4:371–375Google Scholar
  24. 24.
    Machoy-Mokrzyńska A, Borowiak K, Bohatyrewicz A, Janus T (2006) Protective action of milk proteins in rats exposed to fluorine compounds. Acta Toxicologica 14:55–61Google Scholar
  25. 25.
    Jarzynka W, Put A, Ceglecka M (1990) Micromorphology of dentine in rats with chronic exposure to ammonium fluoride. Czas Stomatol 43:255–260PubMedGoogle Scholar
  26. 26.
    McGrath KR, Nakamoto T (1985) Orally administered methionine alters the growth of tooth germs in newborn rats. Ann Nutr Metab 29:374–380PubMedCrossRefGoogle Scholar
  27. 27.
    Birkner E (2002) Influence of antioxidative factors, fluorine and selenium on development of experimental hypercholesterolemia in rabbits. Ann Acad Med Siles 44:1–181Google Scholar
  28. 28.
    Buettner GR (1993) The pecking order of free radicals and antioxidants: lipid peroxidation, a tocopherol, and ascorbate. Arch Biochem Biophys 300:535–543PubMedCrossRefGoogle Scholar
  29. 29.
    Yamashita N, Marata M, Inaue S, Burkitt M, Milne L, Kawanishi S (1998) Alpha-tocopherol induces oxidative damage to DNA in the presence of copper (II) ion. Chem Res Toxicol 11:855–862PubMedCrossRefGoogle Scholar
  30. 30.
    Edwards CE, Gadsden EL, Edwards GA (2009) Utilization of methionine by the adult rat. Early incorporation of methionine-methyl-C14 and methionine-2-C14 into rat tissues. J Nutrition 13:211–216Google Scholar
  31. 31.
    Herrmann M, Wildemann B, Claes L, Klohs S, Ohnmacht M, Taban-Shomal O, Hubner U, Pexa A, Umanskaya N, Herrmann W (2007) Experimental hyperhomocysteinemia reduces bone quality in rats. Clin Chem 53:1455–1461PubMedCrossRefGoogle Scholar
  32. 32.
    Soyupek F, Cerci S, Yildiz S, Yildiz M, Gumus B (2007) Effect of homocysteine on bone mineral density of rats. Biol Trace Elem Res 118:255–259PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Iwona Błaszczyk
    • 1
  • Ewa Birkner
    • 1
  • Izabela Gutowska
    • 2
  • Ewa Romuk
    • 1
  • Dariusz Chlubek
    • 3
  1. 1.Department of BiochemistrySilesian Medical UniversityZabrzePoland
  2. 2.Department of Biochemistry and Human NutritionPomeranian Medical UniversitySzczecinPoland
  3. 3.Department of Biochemistry and Medical ChemistryPomeranian Medical UniversitySzczecinPoland

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