Biological Trace Element Research

, Volume 144, Issue 1–3, pp 1405–1418

Investigations on the Interaction between Cuprous Oxide Nanocubes and Bovine Serum Albumin with Comprehensive Spectroscopic Methods

  • Peng Ju
  • Hai Fan
  • Tao Liu
  • Lin Cui
  • Shiyun Ai
  • Xiaona Wu
Article

Abstract

The interaction between cuprous oxide (Cu2O) nanocubes and bovine serum albumin (BSA) was investigated from a spectroscopic angle under simulative physiological conditions. Under pH 7.4, Cu2O could effectively quench the intrinsic fluorescence of BSA via static quenching. The apparent binding constant (KA) was 3.23, 1.91, and 1.20 × 104 M-1 at 298, 304, and 310 K, respectively, and the number of binding sites was 1.05. According to the Van’t Hoff equation, the thermodynamic parameters (ΔH° = −63.39 kJ mol-1, ΔS° = −126.45 J mol-1 K-1) indicated that hydrogen bonds and van der Waals forces played a major role in stabilizing the BSA–Cu2O complex. Besides, the average binding distance (r0 = 2.76 nm) and the critical energy transfer distance (R0 = 2.35 nm) between Cu2O and BSA were also evaluated according to Förster’s non-radioactive energy transfer theory. Furthermore, UV-visible and circular dichroism results showed that the addition of Cu2O changed the secondary structure of BSA and led to a decrease in α-helix. All results showed that BSA underwent substantial conformational changes induced by Cu2O, which can be very helpful in the study of nanomaterials in the application of biomaterials.

Keywords

Cu2O nanocubes BSA Fluorescence quenching Interaction 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Peng Ju
    • 1
  • Hai Fan
    • 1
  • Tao Liu
    • 1
  • Lin Cui
    • 1
  • Shiyun Ai
    • 1
  • Xiaona Wu
    • 1
  1. 1.College of Chemistry and Material ScienceShandong Agricultural UniversityTaianPeople’s Republic of China

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