Biological Trace Element Research

, Volume 144, Issue 1–3, pp 894–903 | Cite as

Effect of Zinc and Iron Fortification of the Feed on Liver and Thyroid Function in Rats

  • Saeed AkhtarEmail author
  • Faqir M. Anjum
  • Zia Ur Rehman
  • M. Riaz
  • Muhammad Arshad
  • Abdul Basit
  • Tariq Ismail


Changes in plasma glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), triiodothyronine (T3), and thyroxin (T4) of male rats, following 8 weeks of administration of different concentrations of elemental iron (EI), sodium iron ethylenediaminetetraacetate (NaFeEDTA), zinc sulfate (ZnSO4), and zinc oxide (ZnO) in whole wheat flour were investigated. Liver enzymes and thyroid hormones were determined using colorimetric methods and enzyme immune assay, respectively. Ingestion of fortified diets by the male rats did not show a marked effect on GOT and GPT, the exception being NaFeEDTA fed alone and EI with ZnO-fortified diets indicating a significant (p < 0.05) decrease in GOT levels in rats. Similarly, only NaFeEDTA significantly (p < 0.05) increased thyroxin, while EI, ZnSO4, and ZnO either alone or in any combination did not alter thyroid secretions. Changes in GOT, GPT, T3, and T4 levels, as a result of ingesting iron and zinc-fortified diets by rats, ranged in the safe limits.


Fortification Minerals Glutamate oxaloacetate transaminase Glutamate pyruvate transaminase Triiodothyronine Thyroxin 



The authors wish to thank the Higher Education Commission of Pakistan for the financial support to conduct this research. The authors further thank the Head Department of the Food Science and Technology at Bahauddin Zakariya University Multan-Pakistan for providing facilities to carry out a part of the research in this department.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Saeed Akhtar
    • 1
    Email author
  • Faqir M. Anjum
    • 2
  • Zia Ur Rehman
    • 3
  • M. Riaz
    • 1
  • Muhammad Arshad
    • 4
  • Abdul Basit
    • 4
  • Tariq Ismail
    • 1
  1. 1.Department of Food Science and TechnologyBahauddin Zakariya UniversityMultanPakistan
  2. 2.National Institute of Food Science and TechnologyUniversity of AgricultureFaisalabadPakistan
  3. 3.Department of Physiology and PharmacologyUniversity of AgricultureFaisalabadPakistan
  4. 4.Faculty of Veterinary SciencesBahauddin Zakariya UniversityMultanPakistan

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