Biological Trace Element Research

, Volume 144, Issue 1–3, pp 636–646 | Cite as

High Copper Levels Promotes Broiler Hepatocyte Mitochondrial Permeability Transition In Vivo and In Vitro

  • Rongsheng Su
  • Rongmei Wang
  • Huabin Cao
  • Jiaqiang Pan
  • Lijun Chen
  • Chengmei Li
  • Dayou Shi
  • Zhaoxin Tang


This study was to examine the effects of copper on the mitochondrial non-specific pore. Three hundred sixty, one-day-old, healthy Arbor Acres (AA) broilers were fed with different concentrations (11, 110, 220, and 330 mg/kg) of copper originated from copper sulfate, tribasic copper chloride (TBCC), or copper methionine. At the indicated time point, the mitochondrial permeability transition (MPT) and copper concentration were analyzed. Results showed that under the same copper concentration, the MPT of broilers fed copper methionine was the greatest, followed by TBCC, then copper sulfate. The effects of copper on MPT were time- and dose-dependent. Furthermore, in vitro in the presence of K+, 5 μM Cu2+ could cause permeability transition as compared to 10 μM Cu2+ in buffer without K+. Taking these results together, we have shown that hepatocellular MPT may be influenced not only by source and concentration of copper or the raising period of broilers, but also by the existence of K+.


Copper Broiler Mitochondria Mitochondrial permeability transition 



This project was supported by the National Natural Science Foundation of China (No.30871900).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Rongsheng Su
    • 1
  • Rongmei Wang
    • 2
  • Huabin Cao
    • 3
  • Jiaqiang Pan
    • 1
  • Lijun Chen
    • 1
  • Chengmei Li
    • 1
  • Dayou Shi
    • 1
  • Zhaoxin Tang
    • 1
  1. 1.College of Veterinary MedicineSouth China Agricultural UniversityGuangzhouChina
  2. 2.College of Ying Dong bio-engineeringShaoguan UniversityShaoguanChina
  3. 3.College of Animal Science and TechnologyJiangxi Agricultural UniversityNanchangChina

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