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Biological Trace Element Research

, Volume 143, Issue 3, pp 1704–1721 | Cite as

Selenium-Induced Up-Regulation of the Antioxidant Defense and Methylglyoxal Detoxification System Reduces Salinity-Induced Damage in Rapeseed Seedlings

  • Mirza Hasanuzzaman
  • Mohammad Anwar Hossain
  • Masayuki FujitaEmail author
Article

Abstract

The present study investigates the regulatory role of exogenous selenium (Se) in the antioxidant defense and methylglyoxal (MG) detoxification systems in rapeseed seedlings exposed to salt stress. Twelve-day-old seedlings, grown in Petri dishes, were supplemented with selenium (25 μM Na2SeO4) and salt (100 and 200 mM NaCl) separately and in combination, and further grown for 48 h. The ascorbate (AsA) content of the seedlings decreased significantly with increased salt stress. The amount of reduced glutathione (GSH) and glutathione disulfide (GSSG) increased with an increase in the level of salt stress, while the GSH/GSSG ratio decreased. In addition, the ascorbate peroxidase (APX) and glutathione S-transferase (GST) activity increased significantly with increased salt concentration (both at 100 and 200 mM NaCl), while glutathione peroxidase (GPX) activity increased only at moderate salt stress (100 mM NaCl). Glutathione reductase (GR) activity remained unchanged at 100 mM NaCl, while it was decreased under severe (200 mM NaCl) salt stress. Monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II) activities decreased upon the imposition of salt stress, whereas a sharp decrease of these activities was observed under severe salt stress (200 mM NaCl). Concomitant increases in the levels of H2O2 and lipid peroxidation (MDA) were also measured. Exogenous Se treatment alone had little effect on the non-enzymatic and enzymatic components. However, further investigation revealed that Se treatment had a synergistic effect: in salt-stressed seedlings, it increased the AsA and GSH contents; GSH/GSSG ratio; and the activities of APX, MDHAR, DHAR, GR, GST, GPX, CAT, Gly I, and Gly II. As a result, addition of Se in salt-stressed seedlings led to a reduction in the levels of H2O2 and MDA as compared to salt stress alone. These results suggest that the exogenous application of Se rendered the plants more tolerant to salt stress-induced oxidative damage by enhancing their antioxidant defense and MG detoxification systems.

Keywords

Antioxidant defense Ascorbate–glutathione cycle Brassica Glyoxalase enzymes Oxidative stress Salinity Selenium 

Abbreviations

AO

Ascorbate oxidase

APX

Ascorbate peroxidase

AsA

Ascorbic acid

CAT

Catalase

CDNB

1-chloro-2, 4-dinitrobenzene

DHA

Dehydroascorbate

DHAR

Dehydroascorbate reductase

DTNB

5,5′-dithio-bis (2-nitrobenzoic acid)

EDTA

Ethylenediaminetetraacetic acid

Gly I

Glyoxalase I

Gly II

Glyoxalase II

GR

Glutathione reductase

GSH

Reduced glutathione

GSSG

Oxidized glutathione

GPX

Glutathione peroxidase

GST

Glutathione S-transferase

MDA

Malondialdehyde

MDHA

Monodehydroascorbate

MDHAR

Monodehydroascorbate reductase

MG

Methylglyoxal

NTB

2-nitro-5-thiobenzoic acid

ROS

Reactive oxygen species

Se

Selenium

SLG

S-d-lactoylglutathione

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mirza Hasanuzzaman
    • 1
    • 2
  • Mohammad Anwar Hossain
    • 1
    • 3
  • Masayuki Fujita
    • 1
    Email author
  1. 1.Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of AgricultureKagawa UniversityKagawaJapan
  2. 2.Department of AgronomySher-e-Bangla Agricultural UniversityDhakaBangladesh
  3. 3.Department of Genetics and Plant BreedingBangladesh Agricultural UniversityMymensinghBangladesh

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