Prevention Effect in Selenite-Induced Cataract in Vivo and Antioxidative Effects in Vitro of Crataegus pinnatifida Leaves
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Cataract is the leading cause of blindness worldwide. It is a multifactorial disease primarily associated with oxidative stress produced by free radicals. The present study was undertaken to evaluate the anticataract potential of Crataegus pinnatifida (hawthorn tree) leaves extract in selenite-induced cataract in vivo and antioxidant effects in vitro. In vitro antioxidant assay of C. pinnatifida leaves extract on NO production inhibition, aldose reductase inhibition, and O 2 - radical scavenging activities gave the IC50 of 98.3, 89.7, and 5.98 μg/mL, respectively. To characterize some major compounds in C. pinnatifida leaves extract, nine flavonoids were identified via LC–MS/MS qualitative analysis. Based on in vitro screening results, C. pinnatifida leaves extract eye drops in 0.1% hydroxypropyl methyl cellulose solution were prepared to evaluate the anticataract potential in vivo. Administration of C. pinnatifida leaves extract eye drops alternately three times a day in rat pups with selenite-induced oxidative stress significantly increased serum SOD and CAT activities, and tended to reduce MDA level compared with control group. The antioxidant enzyme SOD, CAT, and GSH activities in lens showed a significant increase. These results may be applied in the future for the prevention and treatment of cataracts.
KeywordsCrataegus pinnatifida Flavonoids Selenite-induced oxidative stress NO formation Aldose reductase inhibitor
This research was supported by the Important Drug Development Fund, Ministry of Science and Technology of China (No. 2009ZX09311-002; No. 2009ZX09102-128) and the International Traditional Chinese Medicine Program for Corporation in Science and Technology (No. 2009DFA31070, No. 2007DFC31670).
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