Biological Trace Element Research

, Volume 138, Issue 1–3, pp 238–249 | Cite as

Effects of Extremely Low-Frequency Magnetic Field on Caspase Activities and Oxidative Stress Values in Rat Brain

  • Mehmet Zulkuf Akdag
  • Suleyman Dasdag
  • Engin Ulukaya
  • Ali Kemal Uzunlar
  • Mustafa Ayberk Kurt
  • Abdullah Taşkın


This study was aimed to investigate the effect of extremely low-frequency magnetic field (ELF-MF) on apoptosis and oxidative stress values in the brain of rat. Rats were exposed to 100 and 500 µT ELF-MF, which are the safety standards of public and occupational exposure for 2 h/day for 10 months. Brain tissues were immunohistochemically stained for the active (cleaved) caspase-3 in order to measure the apoptotic index by a semi-quantitative scoring system. In addition, the levels of catalase (CAT), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidative capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were measured in rat brain. Final score of apoptosis and MPO activity were not significantly different between the groups. CAT activity decreased in both exposure groups (p < 0.05), while TAC was found to be lower in ELF 500 group than those in ELF-100 and sham groups (p < 0.05). MDA, TOS, and OSI values were found to be higher in ELF-500 group than those in ELF-100 and sham groups (p < 0.05). In conclusion, apoptosis was not changed by long-term ELF-MF exposure, while both 100 and 500 µT ELF-MF exposure induced toxic effect in the rat brain by increasing oxidative stress and diminishing antioxidant defense system.


Extremely low-frequency magnetic field Oxidative stress Antioxidant enzyme Caspase-3 activities Brain 



Extremely low-frequency magnetic field








Total antioxidative capacity


Total oxidant status


Oxidative stress index


Reactive oxygen substances


Lipid peroxidation


Thiobarbituric acid


International Commission on Non Ionizing Radiation Protection


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Mehmet Zulkuf Akdag
    • 1
  • Suleyman Dasdag
    • 1
  • Engin Ulukaya
    • 2
  • Ali Kemal Uzunlar
    • 3
  • Mustafa Ayberk Kurt
    • 4
  • Abdullah Taşkın
    • 5
  1. 1.Department of BiophysicsMedical School of Dicle UniversityDiyarbakırTurkey
  2. 2.Department of BiochemistryMedical School of Uludag UniversityBursaTurkey
  3. 3.Department of PathologyMedical School of Duzce UniversityDuzceTurkey
  4. 4.Department of AnatomyMedical School of Uludag UniversityBursaTurkey
  5. 5.Department of BiochemistryMedical School of Harran UniversityUrfaTurkey

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