Biological Trace Element Research

, Volume 137, Issue 1, pp 23–39 | Cite as

Chromium III Histidinate Exposure Modulates Gene Expression in HaCaT Human Keratinocytes Exposed to Oxidative Stress

  • Florence Hazane-Puch
  • Rachida Benaraba
  • Kita Valenti
  • Mireille Osman
  • François Laporte
  • Alain Favier
  • Richard A. Anderson
  • Anne-Marie Roussel
  • Isabelle Hininger-Favier
Article
First Online:
Received:
Accepted:

Abstract

While the toxicity of hexavalent chromium is well established, trivalent chromium is an essential nutrient involved in insulin and glucose homeostasis. To study the antioxidant effects of Cr(III)His, cDNA arrays were used to investigate the modulation of gene expression by trivalent chromium histidinate (Cr(III)His) in HaCaT human keratinocytes submitted to hydrogen peroxide (H2O2). Array was composed by a set of 81 expressed sequences tags (ESTs) essentially represented by antioxidant and DNA repair genes. HaCaT were preincubated for 24 h with 50 μM Cr(III)His and were treated with 50 μM H2O2. Total RNAs were isolated immediately or 6 h after the stress. In Cr(III)His preincubated cells, transcripts related to antioxidant family were upregulated (glutathione synthetase, heme oxygenase 2, peroxiredoxin 4). In Cr(III)His preincubated cells and exposed to H2O2, increased expressions of polymerase delta 2 and antioxidant transcripts were observed. Biochemical methods performed in parallel to measure oxidative stress in cells showed that Cr(III)His supplementation before H2O2 stress protected HaCaT from thiol groups decrease and thiobarbituric acid reactive substances increase. In summary, these results give evidence of antioxidant gene expression and antioxidant protection in HaCaT preincubated with Cr(III)His and help to explain the lack of toxicity reported for Cr(III)His.

Keywords

Oxidative stress Hydrogen peroxide Cr(III)His supplementation Gene expression HaCaT TBARS Thiol groups 

Abbreviations

8-oxodGuo

8-Oxo-7-8-dihydro-2′-deoxyguanosine

ACTB

Actin, beta

ANOVA

Analysis of variance

Cr

Chromium

Cr(III)

Trivalent chromium

Cr(III)His

Trivalent chromium histidinate

Cr(VI)

Hexavalent chromium

DMSO

Dimethylsulfoxide

DTNB

5,5′-Dithio-Bis (2-nitrobenzoic acid)

ECM

Extracellular matrix

ESTs

Expressed sequence tags

GADD45A

Growth arrest and DNA-damage-inducible alpha

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GCLM

Glutamate-cysteine ligase, modifier subunit

GPX1

Glutathione peroxidase 1

GSH

Glutathione

GSR

Glutathione reductase

GSS

Glutathione synthetase

GST

Glutathione S-transferase

H2O2

Hydrogen peroxide

HMOX2

Heme oxygenase 2

IKBIA

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

MMP2

Matrix metallopeptidase 2

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NAC

N-acetyl cysteine

NER

Nucleotide excision repair

NFKB1

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NHEJ

Non-homologous end-joining

PBS

Phosphate-buffered saline

PCR

Polymerase chain reaction

PLA2G5

Phospholipase A2, group V

POLD2

Polymerase delta 2

PRDX4

Peroxiredoxin 4

ROS

Reactive oxygen species

RPL32

Ribosomal protein L32

SE

Standard error

-SH

Thiol groups

SHC1

Src homology 2 domain containing transforming protein 1

SOD1

Superoxide dismutase 1

TBARS

Thiobarbituric acid reactive substances

TXN

Thioredoxin

XPC

Xeroderma pigmentosum C

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Notes

Acknowledgements

This study was part of a US Department of Agriculture funded study entitled “Insulin potentiating compounds and antioxidant nutrients in the prevention and alleviation of glucose intolerance and diabetes” and the International Agreement between laboratories from Joseph Fourier University, Grenoble, France and the Beltsville Human Nutrition Research Center entitled “Naturally Occurring Insulin Enhancing Factors”.

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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Florence Hazane-Puch
    • 1
  • Rachida Benaraba
    • 2
  • Kita Valenti
    • 1
  • Mireille Osman
    • 2
    • 3
  • François Laporte
    • 1
  • Alain Favier
    • 1
  • Richard A. Anderson
    • 4
  • Anne-Marie Roussel
    • 2
    • 3
  • Isabelle Hininger-Favier
    • 2
    • 3
    • 5
  1. 1.Département de Biologie Intégrée, Biologie NutritionnelleCentre Hospitalier UniversitaireGrenoble Cedex 09France
  2. 2.LBFAUniversité Joseph FourierGrenoble IFrance
  3. 3.INSERM, U884GrenobleFrance
  4. 4.USDA, ARS, BHNRC, DGILBeltsvilleUSA
  5. 5.LBFA (Laboratoire de Bioénergétique Fondamentale et Appliquée)UFR PharmacieLa TroncheFrance

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