Biological Trace Element Research

, Volume 133, Issue 2, pp 217–226 | Cite as

Effect of Organic Selenium Supplementation on Growth, Se Uptake, and Nutrient Utilization in Guinea Pigs

  • Mahima Chaudhary
  • Anil Kumar GargEmail author
  • Ganesh Kumar Mittal
  • Vishal Mudgal


Forty weaned male guinea pigs (Cavia porcellus) of 152.6 ± 7.96 g mean body weight were divided into four equal groups and fed a common basal diet comprised of 25% ground cowpea (Vigna unguiculata) hay, 30% ground maize (Zea mays) grain, 22% ground gram (Cicer arietinum) grain, 9.5% deoiled rice (Oryza sativa) bran, 6% soybean (Glycine max) meal, 6% fish meal, 1.5% mineral mixture (without Se), and ascorbic acid at 200 mg/kg to meet their nutrient requirements along with 0, 0.1, 0.2, and 0.3 ppm of organic selenium (Se) in groups I, II, III, and IV, respectively. Experimental feeding lasted for a period of 10 weeks, during which, daily feed intake and weekly body weights were recorded. Intake and digestibility of dry matter, organic matter, ether extract, crude fiber, and nitrogen-free extract as well as uptake of calcium and phosphorus were similar (P > 0.05) among the four groups. Feed:gain ratio was also similar (P > 0.05) in the four groups. However, digestibility of crude protein was significantly (P < 0.001) higher in group II supplemented with 0.1 ppm organic Se as compared to other three group. Intake and absorption of Se was significantly (P < 0.001) higher in all the Se supplemented groups as compared to control group. Average daily gain (ADG) was significantly (P < 0.05) higher in group II (3.16 g/day) and III (3.38 g/day) as compared to group I (2.88 g/day). However, ADG in group IV (supplemented 0.3 ppm organic Se) was significantly (P < 0.05) lower (2.83 g/day) than group II and III, but comparable (P > 0.05) to group I. Findings of the present experiment suggests that Se requirements of guinea pigs are ≥0.2 ppm, as supplementation of 0.1 ppm organic Se in the diet (having 0.1 ppm Se) not only enhanced their growth rate but also improved the protein utilization.


Organic selenium Guinea pigs Growth Nutrient utilization Se uptake 


  1. 1.
    Patterson EL, Milstrey R, Stokstad ELR (1957) Effect of selenium in preventing exudative diathesis in chicks. Proc Soc Exp Biol Med 95:617–620PubMedGoogle Scholar
  2. 2.
    Schwarz K, Foltz CM (1957) Selenium as integral part of factor-3 against dietary necrotic liver degeneration. J Am Chem Soc 79:3292–3293CrossRefGoogle Scholar
  3. 3.
    Jianhua H, Ohtsuka A, Hayashi K (2000) Selenium influences growth via thyroid hormone status in broiler chickens. Br J Nutr 84:727–732PubMedGoogle Scholar
  4. 4.
    Beckett GJ, Beddows SE, Morrice PC, Nicol F, Arthur JR (1987) Inhibition of hepatic deiodination of thyroxine is caused by selenium deficiency in rats. Biochem J 248:433–447Google Scholar
  5. 5.
    NRC (1995) Nutrient requirements of laboratory animals. National Academy Press, Washington, DC, USAGoogle Scholar
  6. 6.
    Jensen C, Pallauf J (2007) Estimation of the selenium requirement of growing guinea pigs (Cavia porcellus). J Anim Physiol Anim Nutr 92:481–491CrossRefGoogle Scholar
  7. 7.
    Kumar N (2006) Effect of supplementation of different levels and sources of selenium on the performance of sheep. M.V.Sc., Thesis. IVRI Izatnagar, IndiaGoogle Scholar
  8. 8.
    O’Dell B (1993) University of Missouri, Columbia, MO, Dennis Renner, Sasco Inc., Lincoln, NE, personal communications. In: Nutrient Requirements of Laboratory Animals, Fourth Revised Edition, 1995. The National Academic Press, Washington DCGoogle Scholar
  9. 9.
    Ewan RC (1976) Effect of selenium on nitrogen metabolism. J Nutr 106:702–709PubMedGoogle Scholar
  10. 10.
    Adkins RS, Ewan RC (1984) Effect of supplemental selenium on pancreatic function and nutrient digestibility in the pig. J Anim Sci 58:351–355PubMedGoogle Scholar
  11. 11.
    Glienke LR, Ewan RC (1977) Selenium deficiency in the young pig. J Anim Sci 45:1334–1340PubMedGoogle Scholar
  12. 12.
    Pagan JD, Karnezos P, Kennedy MAP, Currier T, Hoekstra KE (1999) Effect of selenium source on selenium digestibility in exercised thoroughbreds. In: Proceedings, 16th Equine Nutrition and Physiology Society, Raleigh, NC, pp 135–140Google Scholar
  13. 13.
    Schneider BH, Flat WP (1975) The evaluation of feeds through digestibility experiments. The University of Georgia Press, Athens, GA, pp 57–78Google Scholar
  14. 14.
    AOAC (2000) Officials methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
  15. 15.
    Talapatra SK, Ray SN, Sen KC (1940) Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in foodstuffs. Indian J Vet Sci Anim Husb 10:243–246Google Scholar
  16. 16.
    Snedecor GW, Cochran WG (1989) Statistical methods, 8th edn. Iowa State University Press, Ames, IAGoogle Scholar
  17. 17.
    Steel RGD, Torrie JH (1980) Principles and procedures of statistics. A biometrical approach, 2nd edn. McGraw-Hill International Book Company, New Delhi, IndiaGoogle Scholar
  18. 18.
    Cantor AH, Moorhead PD, Musser MA (1982) Comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults. Poult Sci 61:478–484PubMedGoogle Scholar
  19. 19.
    Bunk MJ, Combs GF Jr (1980) Effect of selenium on appetite in the selenium-deficient chick. J Nutr 110:743–749PubMedGoogle Scholar
  20. 20.
    Jullius AD, Marc H, Davies D, Birt F (1983) Toxic effects of dietary selenium in Syrian hamster. Ann Nutr Metab 27:296–305CrossRefGoogle Scholar
  21. 21.
    Echevarria MG, Henry PR, Ammerman CB, Rao PV, Miles RD (1988) Estimation of the relative bioavailability of inorganic selenium sources for poultry. 2. Tissue uptake of selenium from high dietary selenium concentrations. Poult Sci 67:1585–1592PubMedGoogle Scholar
  22. 22.
    Payne RL, Southern LL (2005) Comparison of inorganic and organic selenium sources for broilers. Poult Sci 84:898–902PubMedGoogle Scholar
  23. 23.
    Payne RL, Southern LL (2005) Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium. Poult Sci 84:1268–1276PubMedGoogle Scholar
  24. 24.
    Miller D, Soares JH Jr, Bauersfeld P Jr, Cuppett SL (1972) Comparative selenium retention by chicks fed sodium selenite, selenomethionine, fish meal and fish solubles. Poult Sci 51:1669–1673PubMedGoogle Scholar
  25. 25.
    Mahan DC, Parrett NA (1996) Evaluating the efficiency of selenium-enriched yeast and sodium selenite on tissue selenium retention and serum glutathione peroxidase activity in grower and finisher swine. J Anim Sci 74:2967–2974PubMedGoogle Scholar
  26. 26.
    Daza A, Salado S, Galvez JR, Gutierrez-Barquin M (2000) Effects of vitamin E and selenium supplementation on the immune system, hematological parameters and the performance of weaned pigs. Investig Agrar Prod Sanid Anim 15:21–30Google Scholar
  27. 27.
    Mahan DC, Peters JC (2004) Long-term effects of dietary organic and inorganic selenium sources and levels on reproducing sows and their progeny. J Anim Sci 82:1343–1358PubMedGoogle Scholar
  28. 28.
    Mudgal V, Garg AK, Dass RS, Varshney VP (2008) Effect of selenium and copper supplementation on blood metabolic profile in male buffalo (Bubalus bubalis) calves. Biol Trace Elem Res 121:31–38CrossRefPubMedGoogle Scholar
  29. 29.
    Ivancic J Jr, Weiss WP (2001) Effect of dietary sulfur and selenium concentrations on selenium balance of lactating Holstein cows. J Dairy Sci 84:225–232PubMedCrossRefGoogle Scholar
  30. 30.
    Nicholson JWG, McQueen RE, Bush RS (1991) Response of growing cattle to supplementation with organically bound or inorganic sources of selenium or yeast cultures. Can J Anim Sci 71:803–811Google Scholar
  31. 31.
    White CL, Somers M (1977) Sulphur–selenium studies in sheep I. The effect of varying dietary sulphate and selenomethionine on sulphur, nitrogen and selenium metabolism in sheep. Aust J Biol Sci 30:47–56PubMedGoogle Scholar
  32. 32.
    Tian JZ, Yun MS, Kong CS, Piao LG, Long HF, Kim JH, Lee JH, Lim JS, Kim CH, Kim YY, Han IK (2006) Effects of different products and levels of selenium on growth, nutrient digestibility and selenium retention of growing-finishing pigs. Asian-Australas J Anim Sci 19:61–66Google Scholar
  33. 33.
    Seboussi R, Faye B, Alhadrami G, Askar M, Ibrahim W, Hassan K, Mahjoub B (2008) Effect of different selenium supplementation levels on selenium status in camel. Biol Trace Elem Res 123:124–138CrossRefPubMedGoogle Scholar
  34. 34.
    Mcclure KE, Mahan DC (1988) Effect of dietary selenium source on retention, digestibility and wool accumulation of selenium in growing lambs. Nutr Rep Int 37:839–846Google Scholar
  35. 35.
    Lowry KR, Mahan DC, Corley JR (1985) Effect of dietary calcium on selenium retention in postweaning swine. J Anim Sci 60:1429–1437PubMedGoogle Scholar
  36. 36.
    Choct M, Naylor AJ, Reinke N (2004) Selenium supplementation affects broiler growth performance, meat yield and feather coverage. Br Poult Sci 45:677–683CrossRefPubMedGoogle Scholar
  37. 37.
    Spears JW, Grimes J, Lloyd K, Ward TL (2003) Efficacy of a novel organic selenium compound (zinc-1-selenomethionine, Availa Se) in broiler chicks. In: Proceedings of the 1st Latin American Congress of Animal Nutrition, Cancum, Mexico, pp 197–198Google Scholar
  38. 38.
    Dean WF, Combs GF Jr (1981) Influence of dietary selenium on performance, tissue selenium content, and plasma concentrations of selenium-dependent glutathione peroxidase, vitamin E, and ascorbic acid in ducklings. Poult Sci 60:2655–2663PubMedGoogle Scholar
  39. 39.
    Vignola G, Lambertini L, Mazzone G, Giammarco M, Tassinari M, Martelli G, Bertin G (2009) Effects of selenium source and level of supplementation on the performance and meat quality of lambs. Meat Sci 81:678–685CrossRefGoogle Scholar
  40. 40.
    Siam SS, Mansour KM, El-Anwar EMM, El-Warth AA (2004) Laying hens performance, hatchability, immune response and some blood constituents as affected by vitamin E and selenium supplementation under hot conditions. Egypt Poult Sci J 24:483–496Google Scholar
  41. 41.
    Stone WL, Stewart ME, Nicholas C, Pavuluri S (1986) Effects of dietary selenium and vitamin E on plasma lipoprotein cholesterol levels in male rats. Ann Nutr Metab 30:94–103CrossRefPubMedGoogle Scholar
  42. 42.
    El-Latif SAA, El-Ghamry AA, El-Yamany AT (2004) Effect of using zinc, selenium or vitamin E supplementation on performance and metabolic responses of growing Japanese quail fed diets contaminated with Ochratoxin. Egypt Poult Sci J 24:447–463Google Scholar
  43. 43.
    Wang Y, Han J, Li W, Xu Z (2007) Effect of different selenium source on growth performances, glutathione peroxidase activities, muscle composition and selenium concentration of allogynogenetic crucian carp (Carassius auratus gibelio). Anim Feed Sci Technol 134:243–251CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Mahima Chaudhary
    • 1
    • 2
  • Anil Kumar Garg
    • 1
    Email author
  • Ganesh Kumar Mittal
    • 1
    • 2
  • Vishal Mudgal
    • 1
    • 3
  1. 1.Mineral and Vitamin Nutrition Laboratory, Centre of Advanced Studies in Animal NutritionIndian Veterinary Research InstituteIzatnagarIndia
  2. 2.Animal Nutrition DepartmentVeterinary CollegeMathuraIndia
  3. 3.Animal Nutrition DepartmentVeterinary CollegeJabalpurIndia

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