Effects of Selenium and L-Carnitine on Oxidative Stress in Blood of Rat Induced by 2.45-GHz Radiation from Wireless Devices

  • Nurhan Gumral
  • Mustafa Naziroglu
  • Ahmet Koyu
  • Kurtulus Ongel
  • Omer Celik
  • Mustafa Saygin
  • Mesud Kahriman
  • Sadettin Caliskan
  • Mustafa Kayan
  • Osman Gencel
  • Manuel F. Flores-Arce
Article

Abstract

The levels of blood lipid peroxidation, glutathione peroxidase, reduced glutathione, and vitamin C were used to follow the level of oxidative damage caused by 2.45 GHz electromagnetic radiation in rats. The possible protective effects of selenium and L-carnitine were also tested and compared to untreated controls. Thirty male Wistar Albino rats were equally divided into five groups, namely Groups A1 and A2: controls and sham controls, respectively; Group B: EMR; Group C: EMR + selenium, Group D: EMR + L-carnitine. Groups B–D were exposed to 2.45 GHz electromagnetic radiation during 60 min/day for 28 days. The lipid peroxidation levels in plasma and erythrocytes were significantly higher in group B than in groups A1 and A2 (p < 0.05), although the reduced glutathione and glutathione peroxidase values were slightly lower in erythrocytes of group B compared to groups A1 and A2. The plasma lipid peroxidation level in group A2 was significantly lower than in group B (p < 0.05). Erythrocyte reduced glutathione levels (p < 0.01) in group B; erythrocyte glutathione peroxidase activity in group A2 (p < 0.05), group B (p < 0.001), and group C (p < 0.05) were found to be lower than in group D. In conclusion, 2.45 GHz electromagnetic radiation caused oxidative stress in blood of rat. L-carnitine seems to have protective effects on the 2.45-GHz-induced blood toxicity by inhibiting free radical supporting antioxidant redox system although selenium has no effect on the investigated values.

Keywords

2.45 GHz electromagnetic radiation Oxidative stress Glutathione peroxidase Vitamin C L-carnitine Selenium 

Abbreviations

List of Abbreviations

CAT

catalase

EMR

electromagnetic radiation

GSH

glutathione

GSH-Px

glutathione peroxidase

ip

intraperitoneally

L-CAR

L-carnitine

LP

lipid peroxidation

MDA

malondialdehyde

RF

radio frequency

ROS

reactive oxygen species

SAR

specific absorption rate

Se

selenium

SOD

superoxide dismutase

References

  1. 1.
    Maoquan LI, Yanyan W, Yanwen Z, Zhou Z, Zhengping YU (2008) Elevation of plasma corticosterone levels and hippocampal glucocorticoid receptor translocation in rats: a potential mechanism for cognition impairment following chronic low-power-density microwave exposure. J Radial Res 49:163–170CrossRefGoogle Scholar
  2. 2.
    Koyu A, Nazıroglu M, Ozguner F, Yilmaz HR, Uz E, Cesur G (2005) Caffeic acid phenethyl ester modulates 1800 MHz microwave-ınduced oxidative stress in rat liver. Electromagn Biol Med 24:135–142CrossRefGoogle Scholar
  3. 3.
    Wang J, Sakurai T, Koyama S, Komatubara Y, Suzuki Y, Taki M, Miyakoshi J (2005) Effects of 2450 MHz electromagnetic fields with a wide range of SARs on methylcholanthrene-induced transformation in C3H10T1/2 cells. J Radial Res 46:351–361CrossRefGoogle Scholar
  4. 4.
    Crouzier D, Testylier G, Perrin A, Debouzy JC (2007) Which neurophysiologic effects at low level 2.45 GHz RF exposure? Pathol Biol (Paris) 55:235–41Google Scholar
  5. 5.
    Aweda MA, Gbenebitse, S, Meidinyo RO (2003) Effects of 2.45 GHz microwave exposures on the peroxidation status in Wistar rats. Niger Postgrad Med J 10:243–246PubMedGoogle Scholar
  6. 6.
    Omura Y, Losco M (1993) Electro-magnetic fields in the home environment (color TV, computer monitor, microwave oven, cellular phone, etc) as potential contributing factors for the induction of oncogen C-fos Ab1, oncogen C-fos Ab2, alpha 5 beta 1 and development of cancer, as well as effects of microwave on amino acid composition of food and living human brain. Acupunct Electrother Res 18:33–73PubMedGoogle Scholar
  7. 7.
    Murphy JC, Kaden DA, Warren J, Sivak A (1993) International commission for protection against environmental mutagens and carcinogens. Power frequency electric and magnetic fields: a review of genetic toxicology. Mutat Res 296:221–240PubMedGoogle Scholar
  8. 8.
    Kim MJ, Rhee SJ (2004) Green tea catechins protect rats from microwave-induced oxidative damage to heart tissue. J Med Food 7:299–304CrossRefPubMedGoogle Scholar
  9. 9.
    Moustafa YM, Moustafa RM, Beacy A, Abou-El-Ela SH, Ali FM (2001) Effects of acute exposure to the radiofrequency fields of cellular phones on plasma lipid peroxide and antioxidase activities in human erythrocytes. J Pharm Biomed Anal 26:605–608CrossRefPubMedGoogle Scholar
  10. 10.
    Irmak MK, Fadillioglu E, Gulec M, Erdogan H, Yagmurca M, Akyol O (2002) Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochem Funct 20:279–283CrossRefPubMedGoogle Scholar
  11. 11.
    Naziroglu M, Simsek M, Kutlu M (2004) Moderate exercise with dietary vitamin C and E combination protects streptozotocininduced oxidative damage to the blood and improves fetal outcomes in pregnant rats. Clin Chem Lab Med 42:511–517CrossRefPubMedGoogle Scholar
  12. 12.
    Naziroglu M, Karaoglu A, Aksoy AO (2004) Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicology 195:221–230CrossRefPubMedGoogle Scholar
  13. 13.
    Arrigoni O, De Tullio MC (2002) Ascorbic acid: much more than just an antioxidant. Biochim Biophys Acta 1569:1–9PubMedGoogle Scholar
  14. 14.
    Inazu M, Matsumiya T (2008) Physiological functions of carnitine and carnitine transporters in the central nervous system. Nihon Shinkei Seishin Yakurigaku Zasshi. 28:113–20PubMedGoogle Scholar
  15. 15.
    Dutta A, Ray K, Singh VK, Vats P, Singh SN, Singh SB. (2008) L-carnitine supplementation attenuates intermittent hypoxia induced oxidative stress and delays muscle fatigue in rats. Exp Physiol 93:1139–1146CrossRefPubMedGoogle Scholar
  16. 16.
    Faraone A, Ballen M, Bit-Babik G, Gressner AV, Kanda MY, Swicord ML, Chou CK. (2004). RF dosimetry fort he ferris-wheel mouse exposure system. Motorola Labs Final Report, August.Google Scholar
  17. 17.
    Koylu H, Mollaoglu H, Ozguner F, Naziroglu M, Delibas N (2006) Melatonin modulates 900 MHz microwave-induced lipid peroxidation changes in rat brain. Toxicol Ind Health 22:211–216CrossRefPubMedGoogle Scholar
  18. 18.
    Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of lipid peroxidation (malonyldialdehyde) in biological fluids. Anal Biochem 16:359–364CrossRefPubMedGoogle Scholar
  19. 19.
    Eren I, Naziroglu M, Demirdaş A (2007) Protective effects of lamotrigine, aripirazole and escitalopram on depression- induced oxidative stress in rat brain. Neurochem Res 32:1188–1195CrossRefPubMedGoogle Scholar
  20. 20.
    Sedlak J, Lindsay RHC (1968) Estimation of total, protein bound and non-protein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem 25:192–205CrossRefPubMedGoogle Scholar
  21. 21.
    Naziroglu M (2003) Enhanced testicular antioxidant capacity in streptozotocin induced diabetic rats: protective role of vitamins C, E and selenium. Biol Trace Elem Res 94:61–71CrossRefPubMedGoogle Scholar
  22. 22.
    Lawrence RA, Burk RF. (1971) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958CrossRefGoogle Scholar
  23. 23.
    Cannan RK (1958) Proposal for a certified standard for use in hemoglobinometry; second and final report. Clin Chem 4:246–51PubMedGoogle Scholar
  24. 24.
    Jagota SK, Dani HM (1982) A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent. Anal Biochem 127:178–182CrossRefPubMedGoogle Scholar
  25. 25.
    Braune S, Wrocklage C, Raczek J, Galius T, Lucking CH (1998) Resting blood pressure increase during exposure to a radio frequency electromagnetic field. Lancet 351:1857–1858CrossRefPubMedGoogle Scholar
  26. 26.
    Meral I, Mert H, Mert N, Deger Y, Yoruk I, Yetkin A, Keskin S (2007) Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. Brain Res 1169:120–124.CrossRefPubMedGoogle Scholar
  27. 27.
    Halliwell B, Gutteridge JMC (1984) Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet 1:1396–1397CrossRefPubMedGoogle Scholar
  28. 28.
    Halliwell B, Gutteridge JMC (1999) Free radicals, other reactive species and disease. In: Halliwell B, Gutteridge JMC (eds) Free Radicals in Biology and Medicine, 3rd edn. Oxford University Press, New York, pp 639–645Google Scholar
  29. 29.
    Naziroglu M (2007) New molecular mechanisms on the activation of TRPM2 channels by oxidative stress and ADP-ribose. Neurochem Res 32:1990–2001CrossRefPubMedGoogle Scholar
  30. 30.
    Reznick AZ, Kagan VE, Ramsey R, Tsuchiya M, Khwaja S, Serbinova EA, Packer L (1992) Antiradical effects in L-propionyl carnitine protection of the heart against ischemia-reperfusion injury: the possible role of iron chelation. Arch Biochem Biophys 296:394–401CrossRefPubMedGoogle Scholar
  31. 31.
    Rani PJ, Panneerselvam C (2001) Effect of L-carnitine on brain lipid peroxidation and antioxidant enzymes in old rats. J. Gerontol. A. Biol. Sci. Med. Sci. 2002, 57, 134–137Google Scholar
  32. 32.
    Arockia-Rani PJ, Panneerselvam C. (2001) Carnitine as a free radical scavenger in ageing. Exp Gerontol 36:1713–1726CrossRefPubMedGoogle Scholar
  33. 33.
    Esposti D, Mariani M, Demartini G, Lucini V, Fraschini F, Mancia M (1994) Modulation of melatonin secretion by acetyl-L-carnitine in adult and old rats. J Pineal Res 17:132–136CrossRefPubMedGoogle Scholar
  34. 34.
    Olatunji-Bello II, Reiter RJ (1997) Effect of acute acetyl-l-carnitine treatment on daytime melatonin synthesis in the rat. Afr J Med Sci 26:175–177Google Scholar
  35. 35.
    Pessotto P, Liberati R, Petrella O, Romanelli L, Calvani M, Peluso G (1997) In experimental diabetes the decrease in the eye of lens carnitine levels is an early important and selective event. Exp Eye Res 64:195–201CrossRefPubMedGoogle Scholar
  36. 36.
    Kocer I, Taysi S, Ertekin MV, Karslioglu I, Gepdiremen A, Sezen O, Serifoglu K (2007) The effect of L-carnitine in the prevention of ionizing radiation-induced cataracts: a rat model. Graefes Arch Clin Exp Ophthalmol 245:588–94CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Nurhan Gumral
    • 1
  • Mustafa Naziroglu
    • 2
  • Ahmet Koyu
    • 1
  • Kurtulus Ongel
    • 3
  • Omer Celik
    • 2
  • Mustafa Saygin
    • 1
  • Mesud Kahriman
    • 4
  • Sadettin Caliskan
    • 1
  • Mustafa Kayan
    • 5
  • Osman Gencel
    • 6
  • Manuel F. Flores-Arce
    • 7
  1. 1.Department of Physiology, Medical FacultySuleyman Demirel UniversityIspartaTurkey
  2. 2.Department of BiophysicsSuleyman Demirel UniversityIspartaTurkey
  3. 3.Family MedicineSuleyman Demirel UniversityIspartaTurkey
  4. 4.Department of Electronics and Communication EngineeringSuleyman Demirel UniversityIspartaTurkey
  5. 5.Department of RadiologyIsparta State HospitalIspartaTurkey
  6. 6.Science Institute of Suleyman Demirel UniversityIspartaTurkey
  7. 7.Department of Chemical and Biochemical EngineeringTijuana Institute of TechnologyTijuanaMexico

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