Evaluation of Oxidative Stress, Antioxidant Status and Serum Vitamin C Levels in Cancer Patients

  • Reza MahdaviEmail author
  • Elnaz FaramarziEmail author
  • Ensiyeh Seyedrezazadeh
  • Mohammad Mohammad-zadeh
  • Masoud Pourmoghaddam


Taking into account the importance role of lipid peroxidation and antioxidants in the prevention and incidence of cancer, the present study was carried out to determine oxidative stress, serum total antioxidant (TAS), and vitamin C levels in cancer patients. Malondialdehyde(MDA), total antioxidant status, and vitamin C levels of 57cancer patients aged 19–80 years and 22 healthy subjects (control group) aged 22–76 years were evaluated. Serum concentrations of MDA as thiobarbitaric acid complexes were measured by fluorometry method, the serum TAS by using commercial test kits from Randox Laboratories, and vitamin C by using spectrocolorimetric method. The mean serum MDA concentrations of all cancer groups except lung cancer were significantly higher than control group (P < 0.004). The mean total antioxidant status was insignificantly higher than control group. The mean serum vitamin C level was significantly lower in patients as compared to the healthy subjects (PV < 0.0001). In conclusion, an alteration in the lipid peroxidation with concomitant changes in antioxidant defense system in cancer patients may be due to excessive oxidative stress. Serum low levels of vitamin C in the different type of cancer patients in spite of adequate daily intake may be due to increased utilization to scavenge lipid peroxides as well as their sequestration by tumor cells.


Cancer Serum malondialdehyde Total antioxidant status Vitamin C 



Financial support of Tabriz University of Medical Sciences is gratefully acknowledged.


  1. 1.
    Jemal A, Thomas A, Murray T, Thun M (2002) Cancer statistics, CA. Cancer J Clin 52:23–47CrossRefGoogle Scholar
  2. 2.
    Howe HL, Wingo PA, Thun MJ, Ries LA, Rosenberg HM, Feigal EG (2001) Annual report to the nation on the status of cancer [1973 through 1998], featuring cancers with recent increasing trends. J Natl Cancer Inst 93:824–842PubMedCrossRefGoogle Scholar
  3. 3.
    Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J (2004) Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 266:37–56PubMedCrossRefGoogle Scholar
  4. 4.
    Yeh CC, Hou MF, Tasi S, Lin SK, Hsiao JK, Huang JC et al (2005) Superoxide anion radical, lipid peroxidation and antioxidant status in the blood of patients with breast cancer. Clin Chim Acta 361:104–111PubMedCrossRefGoogle Scholar
  5. 5.
    Pathak SK, Sharma AR, Steward WP, Mellon JK, Griffiths TRL, Gescher AJ (2005) Oxidative stress and cyclooxygenase activity in prostate carcinogenesis: targets for chemopreventive strategies. Eur J Cancer 41:61–70PubMedCrossRefGoogle Scholar
  6. 6.
    Casaril M, Corso F, Corrocher R (1991) Free radicals in human pathology. Recenti Prog Med 82:39–44PubMedGoogle Scholar
  7. 7.
    Aas PA, Otterlei M, Falnes PO, Vågbø CB, Skorpen F, Akbari M et al (2003) Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA. Nature 421:859–863PubMedCrossRefGoogle Scholar
  8. 8.
    Das UN (2002) A radical approach to cancer. Med Sci Monit 8:79–92Google Scholar
  9. 9.
    Ames BN (1998) Micronutrients prevent cancer and delay aging. Toxicol Lett 102/103:5–18CrossRefGoogle Scholar
  10. 10.
    World Cancer Research Fund/American Institute for Cancer Research (1997) Food research and the prevention of cancer: a global perspective. American Institute for Cancer Research, Washington, DCGoogle Scholar
  11. 11.
    Block G, Dietrich M, Norkus EP, Morrow JD, Hudes M, Caan B (2002) Factors associated with oxidative stress in human populations. Am J Epidemiol 156:274–85PubMedCrossRefGoogle Scholar
  12. 12.
    Wasowicz W, Neve J, Peretz A (1993) Optimized steps in fluoromertic determination of thiobarbituric acid-reactive substances in serum importance of extraction PH and influence of sample preservation and storage. Clin Chem 39:2522–2526PubMedGoogle Scholar
  13. 13.
    Burtis CA, Ashwood ER (1999) Tietz textbook of clinical chemistry. Saunders, PhiladelphiaGoogle Scholar
  14. 14.
    Clancy KJ, Davidson NJ (2000) The biology of breast carcinoma. Cancer 97:825–833Google Scholar
  15. 15.
    Rushmore TH, Morton MR, Pickett CB (1992) The antioxidant response element. J Biol Chem 66:11632–11639Google Scholar
  16. 16.
    Senthil K, Aranganathan S, Nalini N (2004) Evidence of oxidative stress in the circulation of ovarian cancer patients. Clin Chim Acta 399:27–32CrossRefGoogle Scholar
  17. 17.
    Manju V, Kalaivaani SJ, Nalini N (2002) Circulation lipid peroxidation and antioxidant status in cervical cancer patients: a case–control study. Clin Biochem 35:621–625PubMedCrossRefGoogle Scholar
  18. 18.
    Beevi SS, Rasheed AM, Geetha A (2004) Evaluation of oxidative stress and nitric oxide levels in patients with oral cavity cancer. Jpn J Clin Oncol 34:379–385PubMedCrossRefGoogle Scholar
  19. 19.
    Hristozov D, Gadjeva V, Vlykova T, Dimiitrov G (2001) Evaluation of oxidative stress in patients with cancer. Arch Physiol Biochem 109:331–336PubMedGoogle Scholar
  20. 20.
    Skrzydlewska E, Sullkowski S, Koda M, Zalewski B, Kanczuga-Koda L, Sulkowska M (2005) Lipid peroxidation and antioxidant status in colorectal cancer. World J Gastroenterol 11:403–406PubMedGoogle Scholar
  21. 21.
    Kumaraguruparan R, Subapriya R, Kabalimoorthy J, Nagini S (2002) Antioxidant profile in the circulation of patients with fibro adenoma and adenocarcinoma of the breast. Clin Biochem 35:275–279PubMedCrossRefGoogle Scholar
  22. 22.
    Prior RL, Cao G (1999) In vivo antioxidant capacity: comparison of different analytical methods. Free Rad Med 27:1173–1181CrossRefGoogle Scholar
  23. 23.
    Khanzode SS, Khanzode SD, Dakhle GN (2003) Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer. Cancer Lett 195:27–31PubMedCrossRefGoogle Scholar
  24. 24.
    Levine M, Katz A, Padayatty S (1999) Vitamin C. In: Shils ME, Olson JA, Shike M, Ross AC (eds) Modern nutrition in health and disease. Williams & Wilkins, Baltimore, pp 507–524Google Scholar
  25. 25.
    Lee WK, Lee HJ, Surh YJ, Lee CY (2003) Vitamin C and cancer chemoprevention. Am J Clin Nutr 78:1074–1078PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Reza Mahdavi
    • 1
    Email author
  • Elnaz Faramarzi
    • 1
    • 5
    Email author
  • Ensiyeh Seyedrezazadeh
    • 2
  • Mohammad Mohammad-zadeh
    • 3
  • Masoud Pourmoghaddam
    • 4
  1. 1.Nutritional Research CenterTabriz University of Medical SciencesTabrizIran
  2. 2.School of Public Health and NutritionTabriz University of Medical SciencesTabrizIran
  3. 3.Department of Radiotherapy Imam Khomeini HospitalTabriz University of Medical SciencesTabrizIran
  4. 4.Faculty of MedicineTabriz University of Medical SciencesTabrizIran
  5. 5.Department of Nutrition, School of Public Health and NutritionTabriz University Medical SciencesTabrizIran

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