Silicon Supplementation Improves the Bone Mineral Density of Calcium-Deficient Ovariectomized Rats by Reducing Bone Resorption

  • Mi-Hyun Kim
  • Yun-Jung Bae
  • Mi-Kyeong Choi
  • Yoon-Sok Chung
Article

Abstract

The purpose of this study was to investigate the effect of silicon (Si) supplementation on bone mineral density (BMD) and bone metabolism parameters relative to calcium (Ca) intake levels in ovariectomized rats. A total of 72 female Wistar rats (6 weeks) were ovariectomized (OVX) and divided into six groups, and Si (500 mg of Si per kilogram of feed) was or was not administered with diets containing various levels of Ca (0.1%, 0.5%, and 1.5%) for 10 weeks. The groups were as follows: (1) Ca-deficient group (0.1% Ca), (2) Ca-deficient with Si supplementation group, (3) adequate Ca group (0.5% Ca), (4) adequate Ca with Si supplementation group, (5) high Ca group (1.5% Ca), and (6) high Ca with Si supplementation group. Si supplementation significantly increased the BMD of the femur and tibia in Ca-deficient OVX rats, while no change was observed with Si supplementation in the BMD of the spine, femur, and tibia in the adequate and high Ca groups. Serum alkaline phosphatase and osteocalcin levels were not affected by Si supplementation or Ca intake levels. C-telopeptide type I collagen levels were significantly decreased as a result of Si supplementation in Ca-deficient OVX rats. In summary, Si supplementation produced positive effects on bone mineral density in Ca-deficient OVX rats by reducing bone resorption. Therefore, Si supplementation may also prove to be helpful in preventing osteoporosis in postmenopausal women whose calcium intake is insufficient.

Keywords

Silicon supplementation Ca deficient Bone mineral density Osteocalcin CTX Urinary calcium Ovariectomy (rat) 

References

  1. 1.
    Albright F, Smith PH, Richardson AM (1941) Postmenopausal osteoporosis. JAMA 116:2465–2474Google Scholar
  2. 2.
    Riggs BL, Wahner HW, Melton LJ 3rd, Richelson LS, Judd HL, Offord KP (1986) Rates of bone loss in the appendicular and axial skeletons of women. Evidence of substantial vertebral bone loss before menopause. J Clin Invest 77:1487–1491PubMedCrossRefGoogle Scholar
  3. 3.
    Prentica A (2004) Diet, nutrition and the prevention of osteoporosis. Public Health Nutr 7:227–243Google Scholar
  4. 4.
    Ministry of Health & Welfare (2006) Report on 2005 national health and nutrition examination survey—health behavior of adults. Ministry of Health & Welfare, Seoul, South KoreaGoogle Scholar
  5. 5.
    Jugdaohsingh R, Anderson SH, Tucker KL, Elliott H, Kiel DP, Thompson RP, Powell JJ (2002) Dietary silicon intake and absorption. Am J Clin Nutr 75:887–893PubMedGoogle Scholar
  6. 6.
    Bae YJ, Kim JY, Choi MK, Chung YS, Kim MH (2008) Short-term administration of water-soluble silicon improves mineral density of the femur and tibia in ovariectomized rats. Biol Trace Elem Res 124(2):157–163 doi:10.1007/s12011-008-8138-3 PubMedCrossRefGoogle Scholar
  7. 7.
    Rico H, Gallego JL, Hernandez ER, Villa LF, Sanchex-Atrio A, Seco C, Gervas JJ (2000) Effect of silicon supplement on osteopenia indced by ovariectomy in rats. Calcif Tissue Int 66:53–55PubMedCrossRefGoogle Scholar
  8. 8.
    Calomme M, Geusens P, Demeester N, Behets GJ, D’Haese P, Sindambiwe JB, Van Hoof V, Berghe DV (2006) Partial prevention of long-term femoral bone loss in aged ovariectomized rats supplemented with choline-stabilized orthosilicic acid. Calcif Tissue Int 78:227–232PubMedCrossRefGoogle Scholar
  9. 9.
    Reeves PG, Nielsen FH, Fahey GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951PubMedGoogle Scholar
  10. 10.
    Rico H, Gómez-Raso N, Revilla M, Hernández ER, Seco C, Páez E, Crespo E (2000) Effects on bone loss of manganese alone or with copper supplement in ovariectomized rats. A morphometric and densitomeric study. Eur J Obstet Gynecol Reprod Biol 90:97–101PubMedCrossRefGoogle Scholar
  11. 11.
    O’Loughlin PD, Morris HA (2003) Oophorectomy acutely increases calcium excretion in adult rats. J Nutr 133:2277–2280PubMedGoogle Scholar
  12. 12.
    Kalu DN, Liu DD, Hardin RR, Hollis BW (1989) The aged rat model of ovarian hormone deficiency bone loss. Endocrinology 124:7–16PubMedCrossRefGoogle Scholar
  13. 13.
    Yamaura M, Nakamura T, Tsurukami H, Hijioka A, Narusawa K, Ohnishi H, Ohta T, Hosoda K (1996) Local bone turnover in the metaphysis of the proximal tibia and the lumbar vertebra during the early periods after ovariectomy in rats. Calcif Tissue Int 58:52–59PubMedCrossRefGoogle Scholar
  14. 14.
    Bae YJ, Sohn EH, Kim BC, Se DHW, Kim MH (2008) The effects of manganese supplementation on bone status and calcium balance in ovariectomized rats according to the calcium intake levels. Korean J Nutr 41:206–215Google Scholar
  15. 15.
    Reffitt DM, Ogston N, Jugdaohsingh R, Cheung HF, Evans BA, Thompson RP, Powell JJ, Hampson GN (2003) Orthosilicic acid stimulates collagen type I synthesis and osteoblastic differentiation in human osteoblastic-like cells in vitro. Bone 32:127–135PubMedCrossRefGoogle Scholar
  16. 16.
    Hott M, de Pollak C, Modrowski D, Marie PJ (1993) Short-term of organic silicon on trabecular bone in mature ovariectomized rats. Calcif Tissue Int 53:174–179PubMedCrossRefGoogle Scholar
  17. 17.
    Manhan LK, Escott-Stump S (2004) Krause’s food nutrition & diet therapy. In: Anderson JJB (ed) Nutrition and bone health. 11th edn. Elsevier, Philadelphia, USA, pp 645–647Google Scholar

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Mi-Hyun Kim
    • 1
  • Yun-Jung Bae
    • 2
  • Mi-Kyeong Choi
    • 3
  • Yoon-Sok Chung
    • 4
  1. 1.Department of Food and NutritionKangwon National UniversitySamcheok-siSouth Korea
  2. 2.Department of Food and NutritionSookmyung Women’s UniversitySeoulSouth Korea
  3. 3.Department of Human Nutrition and Food ScienceChungwoon UniversityChungnamSouth Korea
  4. 4.Department of Endocrinology and MetabolismAjou University School of MedicineSuwonSouth Korea

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