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Biological Trace Element Research

, Volume 128, Issue 3, pp 258–268 | Cite as

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

  • Flávia de Castro Pereira
  • Cesar Augusto Sam Tiago Vilanova-Costa
  • Aliny Pereira de Lima
  • Alessandra de Santana Braga Barbosa Ribeiro
  • Hugo Delleon da Silva
  • Luiz Alfredo Pavanin
  • Elisângela de Paula Silveira-Lacerda
Article

Abstract

Ruthenium complexes have attracted much attention as possible building blocks for new transition-metal-based antitumor agents. The present study examines the mitotoxic and clastogenic effects induced in the root tips of Allium cepa by cis-tetraammine(oxalato)ruthenium(III) dithionate {cis-[Ru(C2O2)(NH3)4]2(S2O6)} at different exposure durations and concentrations. Correlation tests were performed to determine the effects of the time of exposure and concentration of ruthenium complex on mitotic index (MI) and mitotic aberration index. A comparison of MI results of cis-[Ru(C2O2)(NH3)4]2(S2O6) to those of lead nitrate reveals that the ruthenium complex demonstrates an average mitotic inhibition eightfold higher than lead, with the frequency of cellular abnormalities almost fourfold lower and mitotic aberration threefold lower. A. cepa root cells exposed to a range of ruthenium complex concentrations did not display significant clastogenic effects. Cis-tetraammine(oxalato)ruthenium(III) dithionate therefore exhibits a remarkable capacity to inhibit mitosis, perhaps by inhibiting DNA synthesis or blocking the cell cycle in the G2 phase. Further investigation of the mechanisms of action of this ruthenium complex will be important to define its clinical potential and to contribute to a novel and rational approach to developing a new metal-based drug with antitumor properties complementary to those exhibited by the drugs already in clinical use.

Keywords

Cis-tetraammine(oxalato)ruthenium(III) dithionate Cytotoxicity Genotoxicity Allium cepa Ruthenium compounds 

Notes

Acknowledgments

This work was supported by the non-profit institution Research and Projects Financing (FINEP; grant no. 01.06.0941.00/CT-Saúde to Elisângela de Paula Silveira-Lacerda) and by the Brazilian National Counsel of Technological and Scientific Development (CNPq) through fellowships to Flávia de Castro Pereira (grant no. 381302/2007-5), Cesar Augusto Sam Tiago Vilanova-Costa (grant no. 381303/2007-1), and Aliny Pereira de Lima (grant no. 370646/2007-0).

Funding sources

There are no financial or personal interests that might be viewed as inappropriate influences on this work.

Ethical approval

No studies involving human or experimental animals were conducted in this work. Only meristematic cells of the onion (A. cepa) were used. The techniques employed were suggested as models for environmental monitoring (Fiskesjö 1985)

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Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Flávia de Castro Pereira
    • 1
  • Cesar Augusto Sam Tiago Vilanova-Costa
    • 1
  • Aliny Pereira de Lima
    • 1
  • Alessandra de Santana Braga Barbosa Ribeiro
    • 1
  • Hugo Delleon da Silva
    • 1
  • Luiz Alfredo Pavanin
    • 2
  • Elisângela de Paula Silveira-Lacerda
    • 1
  1. 1.Laboratório de Genética Molecular e Citogenética, Instituto de Ciências Biológicas–ICB I–Sala 200Universidade Federal de GoiásGoiâniaBrazil
  2. 2.Instituto de QuímicaUniversidade Federal de Uberlândia–UFUUberlândiaBrazil

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