Biological Trace Element Research

, Volume 126, Supplement 1, pp 57–68 | Cite as

Effects of Dietary Iron Levels on Growth Performance, Hematological Status, Liver Mineral Concentration, Fecal Microflora, and Diarrhea Incidence in Weanling Pigs

  • Seung Hyung Lee
  • Prashant Shinde
  • Jaeyong Choi
  • Munsu Park
  • Seho Ohh
  • Ill Kyong Kwon
  • Son Il Pak
  • Byung Jo Chae
Article

Abstract

An experiment was conducted in weanling pigs (Landrace × Yorkshire × Duroc) to evaluate the effects of dietary iron levels on growth performance, hematological status, liver mineral concentration, fecal microflora, and diarrhea incidence. One hundred and forty-four piglets (initial BW 5.96 ± 0.93kg) were randomly allotted to one of the four dietary treatments on the basis of their body weights. The basal diets for each phase (phase 1: days0 to 14; phase 2: days15 to 28) were formulated to contain minimal Fe and then supplemented with gradient levels of Fe (0, 50, 100, and 250mg/kg) from ferrous sulfate. Feces were collected on days14 and 28 and used for the analysis of microbial count and trace minerals. Eight piglets from each treatment (two piglets per pen) were bled at 0, 7, 14, 21, and 28days to determine their hematological and plasma Fe status. In addition, two piglets from each pen (eight piglets per treatment) were killed at days14 and 28 to determine liver mineral concentrations. Pigs fed supplemental 250ppm Fe showed lowest overall average daily gain (linear, p = 0.036). Diarrhea incidence was linearly increased (p < 0.001) with supplemental Fe level. On days14, coliform population in normal feces was increased (p = 0.036) linearly with supplemental Fe level, and there were higher (p = 0.043) coliform population and lower (p < 0.001) Bifidobacterium spp. in the diarrhea feces. Supplemental Fe linearly (p < 0.05) improved the total red blood cells, hemoglobin, plasma, and liver (p = 0.109) Fe status of pigs and also increased (linear and quadratic, p < 0.001) the fecal excretion of Fe on days14 and 28. It is concluded that increasing the dietary iron levels in piglets improved their hematological status and liver Fe content; however, higher dietary Fe levels might also be associated with the increased diarrhea incidence.

Keywords

Piglets Iron Growth Diarrhea Hematology Fecal microflora 

References

  1. 1.
    Brock JH (1994) Iron in infection, immunity, inflammation and neoplasia. In: Brock JH, Halliday JW, Pippard MJ, Powell LW (eds) Iron metabolism in health and disease. Saunders, London, pp 354–389Google Scholar
  2. 2.
    Rincker MJ, Hill GM, Link JE, Rowntree JE (2004) Effects of dietary iron supplementation on growth performance, hematological status, and whole-body mineral concentrations of nursery pigs. J Anim Sci 82:3189–3197PubMedGoogle Scholar
  3. 3.
    Kernkamp HCH, Clawson AJ, Ferneyhough RH (1962) Preventing iron-deficiency anaemia in baby pigs. J Anim Sci 21:527–532Google Scholar
  4. 4.
    N. R. C. (1998) Nutrient requirements of swine, 10th rev. edn. National Academies Press, Washington, DCGoogle Scholar
  5. 5.
    Pickett RA, Plumlee MP, Smith WH, Beeson WM (1960) Oral iron requirement of the early weaned pig. J Anim Sci 19:1284Google Scholar
  6. 6.
    Alexander TJL (1994) Vaccines against Escherichia coli diseases. In: Gyles CL (ed) Escherichia coli in domestic animals and humans. CAB International, Wallingford, UK, pp 151–170Google Scholar
  7. 7.
    Gyles C (1994) Escherichia coli in domestic animals. CAB International, Wallingford, UKGoogle Scholar
  8. 8.
    Rouault TA (2004) Pathogenic bacteria prefer heme. Science 305:1577–1578PubMedCrossRefGoogle Scholar
  9. 9.
    Weinberg ED (1978) Iron and infection. Microbiol Rev 42:45–66PubMedGoogle Scholar
  10. 10.
    Mathew AG, Franklin MA, Upchurch WG, Chattin SE (1996) Influence of weaning age on ileal microflora and fermentation acids in young pigs. Nutr Res 16:817–827CrossRefGoogle Scholar
  11. 11.
    Miller ER (1978) Biological availability of iron in iron supplements. Feedstuffs 50:20Google Scholar
  12. 12.
    Manner K, Spieler A (1997) Probiotics in piglets—an alternative to traditional growth promoters. Micoecol Ther 26:243–256Google Scholar
  13. 13.
    Taras D, Vahjen W, Macha M, Simon O (2006) Performance, diarrhea incidence, and occurrence of Escherichia coli virulence genes during long-term administration of a probiotic Enterococcus faecium strain to sows and piglets. J Anim Sci 84:608–617PubMedGoogle Scholar
  14. 14.
    A. O. A. C. (2000) Official methods of analysis, 17th edn. USDA, Gaithersburg, MDGoogle Scholar
  15. 15.
    Knabe DA, LaRue DC, Gregg EJ, Martinez GM, Tanksley TD Jr (1989) Apparent digestibility of nitrogen and amino acids in protein feedstuffs by growing pigs. J Anim Sci 67:441–458PubMedGoogle Scholar
  16. 16.
    Moore S (1963) On the determination of cystine as cysteric acid. J Biol Sci 38:235–237Google Scholar
  17. 17.
    Miltenburg GA, Wensing T, van de Broek J, Mevius DJ, Breukink HJ (1992) Effects of different iron contents in the milk replacer on the development of iron deficiency anemia in veal calves. Vet Q 14:18–21PubMedGoogle Scholar
  18. 18.
    Olson AD, Hamlin WB (1969) A new method for serum iron and total iron-binding capacity by atomic absorption spectrophotometry. Clin Chem 15:438–444PubMedGoogle Scholar
  19. 19.
    Torrallardona D, Conde MR, Badiola I, Polo J, Brufau J (2003) Effect of fishmeal replacement with spray-dried animal plasma and colistin on intestinal structure, intestinal microbiology, and performance of weanling pigs challenged with Escherichia coli K99. J Anim Sci 81:1220–1226PubMedGoogle Scholar
  20. 20.
    Dove CR, Ewan RC (1990) Effect of excess dietary copper, iron or zinc on the tocopherol and selenium status of growing pigs. J Anim Sci 68:2407–2413PubMedGoogle Scholar
  21. 21.
    O’Donovan PB, Pickett RA, Plumlee MP, Beeson WM (1963) Iron toxicity in the young pig. J Anim Sci 22:1075–1080Google Scholar
  22. 22.
    Talbot RB, Swenson MJ (1970) Blood volume of pigs from birth through 6 weeks of age. Am J Physiol 218:1141–1144PubMedGoogle Scholar
  23. 23.
    Amine EK, Raymond N, Hegsted DM (1972) Biological estimation of available iron using chicks or rats. J Agric Food Chem 20:246–251PubMedCrossRefGoogle Scholar
  24. 24.
    Dove CR, Haydon KD (1991) The effect of copper addition to diets with various iron levels on the performance and hematology of weanling swine. J Anim Sci 69:2013–2019PubMedGoogle Scholar
  25. 25.
    Underwood EJ, Suttle NF (1999) The mineral nutrition of livestock, 3rd edn. CABI Publishing, New YorkGoogle Scholar
  26. 26.
    Zimmerman DR (1980) Iron in swine nutrition. In: National feed ingredient association literature review on iron in animal and poultry nutrition. National Feed Ingredients Association, Des Moines, IA, pp 1–56Google Scholar
  27. 27.
    Withrow G, Bell MC (1969) Erythrocytic life span estimations in growing sheep and swine using 75Se. J Anim Sci 28:240–245PubMedGoogle Scholar
  28. 28.
    Schreiber WE (1989) Iron, porphyrin, and bilirubin metabolism. In: Kaplan LA, Pesce AJ (eds) Clinical chemistry. Theory, analysis, and correlation. Mosby, St. Louis, MO, pp 496–511Google Scholar
  29. 29.
    Rincker MJ, Hill GM, Link JE, Meyer AM, Rowntree JE (2005) Effects of dietary zinc and iron supplementation on mineral excretion, body composition, and mineral status of nursery pigs. J Anim Sci 83:2762–2744PubMedGoogle Scholar
  30. 30.
    Zinn KR, Chaudhuri TR, Mountz JM, van den Berg GJ, Gordon DT, Johanning GL (1999) 59Fe is retained from an elemental 59Fe powder supplement without effects on 65zinc, 47calcium and 67copper in young pigs. J Nutr 129:181–187PubMedGoogle Scholar
  31. 31.
    van Beers-Schreurs HMG, Nabuurs MJA, Vellenga L, Kalsbeek-van der Valk HJ, Wensing T, Breukink HJ (1998) Weaning and the weanling diet influence the villous height and crypt depth in the small intestine of pigs and alter the concentrations of short-chain fatty acids in the large intestine and blood. J Nutr 128:947–953PubMedGoogle Scholar
  32. 32.
    Pulske JR, Hampson DJ, Williams IJ (1997) Factors influencing the structure and function of the small intestine in weaned pig: a review. Livest Prod Sci 51:215–236CrossRefGoogle Scholar
  33. 33.
    Nagy B, Arp LH, Moon HW, Casey TA (1992) Colonization of the small intestine of weaned pigs by enterotoxigenic Escherichia coli that lack known colonization factors. Vet Pathol 29:239–246PubMedCrossRefGoogle Scholar
  34. 34.
    Mathew AG, Franklin MA, Upchurch WG, Chattin SE (1996) Influence of weaning age on ileal microflora and fermentation acids in young pigs. Nutr Res 16:817–827CrossRefGoogle Scholar
  35. 35.
    Gera T, Sachdev HPS (2002) Effects of iron supplementation on incidence of infectious illness in children: systematic review. Br Med J 325:1142–1152CrossRefGoogle Scholar
  36. 36.
    Imbert M, Blondeau R (1998) On the iron requirement of lactobacilli grown in chemically defined medium. Curr Microbiol 37:64–66PubMedCrossRefGoogle Scholar
  37. 37.
    Weinberg ED (1997) The Lactobacilli anomaly: total iron abstinence. Perspect Biol Med 40:1–6Google Scholar

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Seung Hyung Lee
    • 1
  • Prashant Shinde
    • 1
  • Jaeyong Choi
    • 1
  • Munsu Park
    • 1
  • Seho Ohh
    • 1
  • Ill Kyong Kwon
    • 1
  • Son Il Pak
    • 2
  • Byung Jo Chae
    • 1
  1. 1.Department of Animal Resource Science, College of Animal Life SciencesKangwon National UniversityChuncheonRepublic of Korea
  2. 2.School of Veterinary MedicineKangwon National UniversityChuncheonKorea

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