Biological Trace Element Research

, Volume 126, Issue 1–3, pp 83–91 | Cite as

MRI-based Visualization of Iron-labeled CD133+ Human Endothelial Progenitor Cells

Article

Abstract

The objective of this study is to build up a kind of effective approach to multiply CD133+ endothelial progenitor cells (EPCs) and visualize cells by labeling with two FDA-approved agents based on MRI technique. CD133+ cells were separated by immunomagnetic microbeads selection and grew with serum-free medium. Seven days later, CD133+ cell production was collected and co-incubated with iron complex for 24 h for labeling. The iron-labeled cells were suspended into agarose gel and scanned by MRI for visualization. Labeled cells were also analyzed for cell viability. Iron can be effectively introduced into CD133+ EPCs plasma in culture and visualized by changing the MRI signal intensity. Iron had no influence on cell viability. Conclusion: Iron substance can be applied to label CD133+ cells without cytotoxicity and iron-labeled cells can be visualized by MRI image. Due to the non-invasive property and repeatability of MRI technology and this kind of method could be used for tracing in vivo stem cells in the future.

Keywords

Endothelial progenitor cells Iron MRI Stem cell visualization 

References

  1. 1.
    Asahara T, Murohara T, Sullivan A et al (1997) Isolation of putative progenitor endothelial cells for angiogenesis. Science 275:964–967PubMedCrossRefGoogle Scholar
  2. 2.
    Khakoo AY, Finkel T (2005) Endothelial progenitor cells. Annu Rev Med 56:79–101PubMedCrossRefGoogle Scholar
  3. 3.
    Lapergue B, Mohammad A, Shuaib A (2007) Endothelial progenitor cells and cerebrovascular diseases. Prog Neurobiol 83:349–362PubMedCrossRefGoogle Scholar
  4. 4.
    Boodhwani M, Sodha NR, Mieno S et al (2007) Functional, cellular, and molecular characterization of the angiogenic response to chronic myocardial ischemia in diabetes. Circulation 116(11 Suppl):I31–37PubMedGoogle Scholar
  5. 5.
    Asahara T, Kawamoto A (2004) Endothelial progenitor cells for postnatal vasculogenesis. Am J Physiol Cell Physiol 287:C572–579PubMedCrossRefGoogle Scholar
  6. 6.
    Si Z, Liu J (2008) What “helps” tumors evade vascular targeting treatment? Chin Med J 121:844–849PubMedGoogle Scholar
  7. 7.
    Lyden D, Hattori K, Dias S et al (2001) Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med 7:1194–1201PubMedCrossRefGoogle Scholar
  8. 8.
    Shaked Y, Ciarrocchi A, Franco M et al (2006) Therapy-induced acute recruitment of circulating endothelial progenitor cells to tumors. Science 313:1785–1787PubMedCrossRefGoogle Scholar
  9. 9.
    Cho HJ, Lee N, Lee JY et al (2007) Role of host tissues for sustained humoral effects after endothelial progenitor cell transplantation into the ischemic heart. J Exp Med 204:3257–3269PubMedCrossRefGoogle Scholar
  10. 10.
    Reimer P, Balzer T (2003) Ferucarbotran (Resovist): a new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver: properties, clinical development, and applications. Eur Radiol 13:1266–1276PubMedGoogle Scholar
  11. 11.
    Matuszewski L, Persigehl T, Wall A et al (2006) Cell tagging with clinically approved iron oxides: feasibility and effect of lipofection, particle size, and surface coating on labeling efficiency. Radiology 235:155–161CrossRefGoogle Scholar
  12. 12.
    Frank JA, Miller BR, Arbab AS et al (2003) Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology 228:480–487PubMedCrossRefGoogle Scholar
  13. 13.
    Mai XL, Teng GJ, Ma ZL et al (2007) In vitro MR imaging of Fe(2)O(3)-PLL labelled rabbit peripheral blood endothelial progenitor cells. Chin J Cardiol 35:838–843Google Scholar
  14. 14.
    Rafii S, Lyden D (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med 9:702–712PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  1. 1.Department of Oncology, Guang An Men HospitalChina Academy of Chinese Medical SciencesBeijingChina
  2. 2.Shandong Medical Imaging Research InstituteShandong UniversityJinanChina

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