Selenium Induces Manganese-dependent Peroxidase Production by the White-Rot Fungus Bjerkandera adusta (Willdenow) P. Karsten
- 120 Downloads
In this study, selenium (Se) induction of the ligninolytic enzyme manganese-dependent peroxidase (MnP) production, and the effects on the oxidative state in the white-rot fungus Bjerkandera adusta (Willdenow) P. Karsten were demonstrated. Low concentration of Se (0.5 mM) caused up to a twofold increase in MnP production (0.81 ± 0.05 U/ml) when compared to control (0.39 ± 0.07 U/ml), whereas higher concentrations of Se (200 mM) inhibited (0.03 ± 0.01 U/ml) MnP production. Addition of high concentration of Se also caused up to a twofold increase in lipid peroxidation levels. These results demonstrate for the first time that Se may induce or reduce MnP production and lipid peroxidation levels which play a significant role in lignin degradation by white-rot fungi.
KeywordsLipid peroxidation Manganese peroxidase Selenium White-rot fungi
This work was funded by Turkish State Planning Organization project titled “Advanced Technologies in Engineering”. We would also like to thank Pinar Huner for her valuable help in MnP stability studies. The authors also thank Kelsey Fisher for her help in proof correction.
- 1.Brigham JS, Adney WS, Himmel ME (1996) Hemicelluloses: diversity and applications. In: Wyman CE (ed) Handbook on bioethanol: production and utilization. Taylor and Francis, Washington, DC, USA, pp 119–142Google Scholar
- 18.Levin L, Forchiassin F, Papinutti L (2002) Effect of copper on the ligninolytic activity of Trametes trogii. Int Biodeterior Biodegrad 49:60Google Scholar