Biological Trace Element Research

, Volume 121, Issue 3, pp 243–248 | Cite as

Effects of Restraint Stress on Iron, Zinc, Calcium, and Magnesium Whole Blood Levels in Mice

  • Wen-feng Teng
  • Wei-min Sun
  • Li-fei Shi
  • Dian-dong Hou
  • Hui LiuEmail author



Study the effects of acute and chronic restraint stress on the whole blood concentrations of iron (Fe), zinc (Zn), calcium (Ca), and magnesium (Mg) in mice.

Materials and methods

Single or repeat restraints were applied to mice to induce acute or chronic stress. The levels of elements in whole blood were determined by flame atomic absorption spectrometry.


The levels of Fe, Zn, Ca, and Mg in blood in the acute-stress group were 351, 5.05, 60, and 44 μg/ml, respectively, and those in the corresponding control group were 391, 5.90, 59, and 45 μg/ml, respectively. The levels of blood Fe, Zn, Ca, and Mg in the chronic-tress group were 291, 3.62, 59, and 40 μg/ml, respectively, and those in the corresponding control group were 393, 4.82, 48, and 43 μg/ml, respectively. The levels of Fe and Zn in the blood of both the acute-stress and the chronic-stress groups were significantly lower (P <0.05) than that in the control groups. The Ca level in whole blood was significantly (P <0.05) higher in the chronic-stress group than that in the control group.


Acute and chronic restraint stress can cause changes in blood levels of Fe and Zn in mice.


Stress Fe Zn Ca Mg Whole blood 



The study was supported by grant NSFC-30370484 (Liu) from the National Natural Science Foundation of China.


  1. 1.
    Gold SM, Mohr DC, Huitinga I, Flachenecker P, Sternberg EM, Heesen C (2005) The role of stress-response systems for the pathogenesis and progression of MS. Trends Immunol 26(12):644–652PubMedCrossRefGoogle Scholar
  2. 2.
    Starkie RL, Hargreaves M, Rolland J, Febbraio MA (2005) Heat stress, cytokines, and the immune response to exercise. Brain Behav Immun 19(5):404–412PubMedCrossRefGoogle Scholar
  3. 3.
    Holsboer F (2001) Stress, hypercortisolism and corticosteroid receptors in depression: implications for therapy. Affect Disord 62(1–2):77–91CrossRefGoogle Scholar
  4. 4.
    Reiche EM, Morimoto HK, Nunes SM (2005) Stress and depression-induced immune dysfunction: implications for the development and progression of cancer. Int Rev Psychiatry 17(6):515–527PubMedCrossRefGoogle Scholar
  5. 5.
    Brown MR, Smith AW (2001) Dormancy and persistence in chronic infection: role of the general stress response in resistance to chemotherapy. J Antimicrob Chemother 48(1):141–142PubMedCrossRefGoogle Scholar
  6. 6.
    Apostoli P (2002) Elements in environmental and occupational medicine. J Chromatogr B Analyt Technol Biomed Life Sci 778(1–2):63–97PubMedGoogle Scholar
  7. 7.
    Ishihara Y, Iijima H, Yagi Y, Hoshi H, Matsunaga K (2004) Inhibition of decrease in natural killer cell activity in repeatedly restraint-stressed mice by a biological response modifier derived from cultured mycelia of the basidiomycete Tricholoma matsutake. Neuroimmunomodulation 11(1):41–48PubMedCrossRefGoogle Scholar
  8. 8.
    Markova OO, Mysula IR, Bodnar Kvik I II, Borys AP (1995) The content of macro- and trace elements in the cardiac muscle of adult and old rats after stressor influence. Fiziol Zh 41(1–2):100–104PubMedGoogle Scholar
  9. 9.
    Okur H, Kucukaydin M, Ustdal KM (1995) The endocrine and metabolic response to surgical stress in the neonate. J Pediatr Surg 30(4):626–625PubMedCrossRefGoogle Scholar
  10. 10.
    Hidalgo J, Garcia A, Oliva AM, Giralt M, Gasull T, Gonzalez B, Milnerowicz H, Wood A, Bremner I (1994) Effect of zinc, copper and glucocorticoids on metallothionein levels of cultured neurons and astrocytes from rat brain. Chem Biol Interact 93(3):197–219PubMedCrossRefGoogle Scholar
  11. 11.
    Wysocki A, Beben P, Winiarski M (1998) The effect of surgical trauma on the level of plasma iron. Przegl Lek 55(10):508–511PubMedGoogle Scholar
  12. 12.
    Luo W, Chen J, Jiang H, Chen Y (2004) Effects of zinc on content of uncoupling protein in cold stress rats. Wei Sheng Yan Jiu 33(1):33–35PubMedGoogle Scholar
  13. 13.
    Fabris N, Mocchegiani E (1995) Zinc, human diseases and aging. Aging (Milano) 7(2):77–93Google Scholar
  14. 14.
    Lieu PT, Heiskala M, Peterson PA, Yang Y (2001) The roles of iron in health and disease. Mol Aspects Med 22(1–2):1–87PubMedCrossRefGoogle Scholar
  15. 15.
    Takase B, Akima T, Uehata A, Ohsuzu F, Kurita A (2004) Effect of chronic stress and sleep deprivation on both flow-mediated dilation in the brachial artery and the intracellular magnesium level in humans. Clin Cardiol 27(4):223–227PubMedCrossRefGoogle Scholar
  16. 16.
    Liashenko VP, Lukashov SM (2003) Effect of stress on calcium homeostasis in muscular tissues. Fiziol Zh 49(5):76–81PubMedGoogle Scholar
  17. 17.
    Joels M, Velzing E, Nair S, Verkuyl JM, Karst H (2003) Acute stress increases calcium current amplitude in rat hippocampus: temporal changes in physiology and gene expression. Eur J Neurosci 18(5):1315–1324PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Wen-feng Teng
    • 1
  • Wei-min Sun
    • 1
  • Li-fei Shi
    • 1
  • Dian-dong Hou
    • 1
  • Hui Liu
    • 1
    Email author
  1. 1.College of Medical LaboratoryDalian Medical UniversityDalianChina

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