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In Vitro Activities of Tigecycline in Combination with Amikacin or Colistin Against Carbapenem-Resistant Acinetobacter baumannii

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Abstract

Carbapenem-resistant Acinetobacter baumannii (CRAB) has been a common pathogen of nosocomial infections and severely threatened the public health for decades. Tigecycline is a new type of antibacterial glycylcycline and minocycline derivative and has been used to treat CRAB in clinical practice. However, the synergistic effects of tigecycline in combination with other antibiotics including colistin or amikacin remain unclear. A total of 216 CRAB isolates were collected from multiple body parts of different patients. The gene types of these isolates were analyzed and their resistance to carbapenems was determined by Etest. Broth microdilution method was utilized to evaluate the minimum inhibitory concentration (MIC) of each sample. Checkerboard screening technique was performed to demonstrate the synergistic effects of antibiotics and fractional inhibitory concentration index (FICI) was established. Therefore, the joint treatment of tigecycline and colistin (1:1) could effectively improve the sensitivity of AB to antibiotics. OXA-24-like isolates were more sensitive to the combination of tigecycline and amikacin. On the other hand, OXA-23-like isolates were more sensitive to the combination of tigecycline and colistin. Tigecycline exhibited synergistic effects with amikacin and colistin to inhibit CRAB.

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Data Availability

Data is available from the authors by request.

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Funding

The study was supported by the Science and Technology Project of Binhai New Area Health Bureau (2013BWKY022).

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Authors and Affiliations

  1. The Clinical Laboratory, Tianjin Fifth Central Hospital, No. 41 Zhejiang Road, Binhai New District, Tianjin, 300450, China

    Hongbin Wu, Heqiang Feng, Lijie He & Heping Zhang

  2. The Clinical Pharmacy, Tianjin Fifth Central Hospital, No. 41 Zhejiang Road, Binhai New District, Tianjin, 300450, China

    Ping Xu

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Contributions

Data curation, analysis: Hongbin Wu, Heqiang Feng, Lijie He, Heping Zhang, and Ping Xu; Drafting of the manuscript: Hongbin Wu and Heqiang Feng; Concept, design of the study: Hongbin Wu and Heqiang Feng. All authors approved the publication of the manuscript.

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Correspondence to Hongbin Wu.

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The study was approved by the Ethics Committee of Tianjin Fifth Central Hospital.

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Hongbin Wu and Heqiang Feng contributed equally to this paper.

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Wu, H., Feng, H., He, L. et al. In Vitro Activities of Tigecycline in Combination with Amikacin or Colistin Against Carbapenem-Resistant Acinetobacter baumannii. Appl Biochem Biotechnol 193, 3867–3876 (2021). https://doi.org/10.1007/s12010-021-03664-z

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