Enhanced Lipid Production in Yarrowia lipolytica Po1g by Over-expressing lro1 Gene under Two Different Promoters
Yarrowia lipolytica is a well-known oleaginous yeast that naturally accumulates lipids to more than 20% of their dry cell weight. Due to its brief doubling time and Generally Recognized as Safe (GRAS) properties, Y. lipolytica has been exploited for the production of commercially valuable lipids. Among the genes related to the lipid synthesis, the gene YALI0E16797g (LRO1) encoding a major triacylglycerol synthase of Y. lipolytica shows a significant impact during the acylation process. Thus, in the present work, we explore the contributions of hp4d or TEFintron promoters to the response of LRO1 expression on lipid accumulation by molecular cloning technology. Results showed that over-expression of LRO1 led to higher lipid content as well as lipid yield. The one with the hp4d promoter showed the highest lipid content of 12% wt. However, such an enhancement also caused a growth defect of cells. On the other hand, the lipid content of the cells over-expressing LRO1 with TEFintron promoter revealed only a modest increase in lipid content, but it promoted cell growth. Therefore, all things considered the one with the TEFintron promoter showed the highest lipid yield.
KeywordsYarrowia lipolytica Lipid Biodiesel Promoter Phospholipid diacylglycerol acyltransferase
This work was supported by the Ministry of Science and Technology, Taiwan (grant numbers MOST 107-2221-E-011-002-MY3 and MOST 105-2221-E-011 -002 -MY3).
Compliance with Ethical Standards
This article does not contain any studies involving human participants or animals performed by any of the authors. No informed consent.
Conflict of Interest
The authors declare that they have no conflicts of interest.
- 4.Windahl, K., Faxen Irving, G., Almquist, T., Liden, M. K., van de Luijtgaarden, M., Chesnaye, N. C., Voskamp, P., Stenvinkel, P., Klinger, M., Szymczak, M., Torino, C., Postorini, M., Drechsler, C., Caskey, F. J., Wanner, C., Dekker, F. W., Jager, K. J., & Evans, M. (2018). Journal of Renal Nutrition, 28, 165–174.CrossRefGoogle Scholar
- 10.Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H., & Stymne, S. (2000). Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proceedings of the National Academy of Sciences of the United States of America, 97(12), 6487–6492.CrossRefGoogle Scholar