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Kinetic and Thermodynamic Investigation of Human Serum Albumin Interaction with Anticancer Glycine Derivative of Platinum Complex by Using Spectroscopic Methods and Molecular Docking

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Abstract

In this paper, a new anticancer Pt (II) complex, cis-[Pt (NH3)2(tertpentylgly)]NO3, was synthesized with glycine-derivative ligand and characterized. Cytotoxicity of this water-soluble Pt complex was studied against human cancer breast cell line of MCF-7. The interaction of human serum albumin (HSA) with Pt complex was studied by using UV-Vis, fluorescence spectroscopy methods, and molecular docking at 27 and 37 °C in the physiological situation (I = 10 mM, pH = 7.4). The negative \( \varDelta {H}_b^0 \) and positive \( \varDelta {S}_b^0 \) indicated that electrostatic force may be a major mode in the binding between Pt complex and HSA. Binding constant values were obtained through UV-Vis and fluorescence spectroscopy that reveal strong interaction. The negative Gibbs free energy that was obtained by using the UV-Vis method offers spontaneous interaction. Fluorescence quenching the intensity of HSA by adding Pt complex confirms the static mode of interaction is effective for this binding process. Hill coefficients, nH, Hill constant, kH, complex aggregation number around HSA, <J>, number of binding sites, g, HSA melting temperature, Tm, and Stern-Volmer constant, kSV, were also obtained. The kinetics of the interaction was studied, which showed a second-order kinetic. The results of molecular docking demonstrate the position of binding of Pt complex on HSA is the site I in the subdomain IIA.

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Abbreviations

HSA:

Human serum albumin

PDB:

Protein Data Bank

Tertpentylgly:

1,1-Dimethyl propyl glycine

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Acknowledgments

The authors of this article would like to thank Payame Noor University of Isfahan.

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Authors and Affiliations

  1. Department of Chemistry, Payame Noor University (PNU), Tehran, Iran

    Afrooz Shiekhzadeh, Nasrin Sohrabi & Mohsen Oftadeh

  2. Chemistry & Chemical Engineering Research Center of Iran, Tehran, Iran

    Mahboube Eslami Moghadam

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  1. Afrooz Shiekhzadeh
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Shiekhzadeh, A., Sohrabi, N., Moghadam, M.E. et al. Kinetic and Thermodynamic Investigation of Human Serum Albumin Interaction with Anticancer Glycine Derivative of Platinum Complex by Using Spectroscopic Methods and Molecular Docking. Appl Biochem Biotechnol 190, 506–528 (2020). https://doi.org/10.1007/s12010-019-03078-y

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