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Use of an (Hemi) Cellulolytic Enzymatic Extract Produced by Aspergilli Species Consortium in the Saccharification of Biomass Sorghum

  • Beatriz Vieira dos Santos
  • Patrísia Oliveira Rodrigues
  • Carlos Juliano Brant Albuquerque
  • Daniel Pasquini
  • Milla Alves BaffiEmail author
Article
  • 24 Downloads

Abstract

This study evaluated the production of lignocellulose-degrading enzymes by solid-state fermentation (SSF) using a microbial consortium of Aspergillus fumigatus SCBM6 and A. niger SCBM1 (AFN extract). The fungal strains were cultivated in sugarcane bagasse (SCB) and wheat bran (WB) as lignocellulosic substrates for 7 days at 30 °C. After SSF, the highest peaks of enzyme production were 150 and 80 U g−1 for β-xylosidase and β-glucosidase at 48 h, 375 U g−1 for xylanase at 96 h, and 80 U g−1 for endoglucanase and 4 U g−1 for cellulase activity on filter paper (FPase) at 144 h. The efficiency of the produced AFN extract was investigated in the enzymatic hydrolysis of crude biomass sorghum (BS) and after the removal of extractives (ES). After saccharification, the glucose and xylose concentrations were 10× superior in ES than in BS hydrolysate (2.5 g L−1 after 12 h). The presence of inhibitors of alcoholic fermentation, such as formic acid, was also reduced in ES hydrolysates, indicating that the removal of extractives positively contributed to the effectiveness of enzymatic hydrolysis of biomass sorghum using AFN extract.

Keywords

Cellulases Hemicellulases Aspergillus Biomass sorghum Enzymatic hydrolysis 

Notes

Acknowledgments

The authors thank to Dr. Bruno Eduardo Lobo Baeta for the assistance in chemical characterization of sorghum biomass.

Funding Information

This study received financial support from Minas Gerais State Foundation for Research Development-FAPEMIG (PRONEM-FAPEMIG-APQ-01360-14).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Agricultural Sciences Institute (ICIAG-UFU)Uberlândia Federal UniversityUberlândiaBrazil
  2. 2.Agricultural Sciences Institute (ICA-UFMG)Minas Gerais Federal UniversityMontes ClarosBrazil
  3. 3.Chemical Institute (IQ-UFU)Uberlândia Federal UniversityUberlândiaBrazil

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