Isolation of Bisphenol A-Tolerating/degrading Shewanella haliotis Strain MH137742 from an Estuarine Environment

  • Felipe Silva de SantanaEmail author
  • Louise Hase Gracioso
  • Bruno Karolski
  • Marcela dos Passos Galluzzi Baltazar
  • Maria Anita Mendes
  • Claudio Augusto Oller do Nascimento
  • Elen Aquino Perpetuo


The human exposure to bisphenol A (BPA) occurs frequently. Once, this compound was one of the highest volume chemicals produced worldwide and used as a plasticizer in many products. However, even at low concentration, it can cause severe damage to the endocrine system because of its endocrine disruptor activity. Thus, mitigation studies to remove or reduce this contaminant from the environment are essential. An alternative method of removing it from the environment is the use of bioremediation processes to the selected bacteria isolated from a BPA-impacted area. In this work, four halotolerant strains were isolated from the Santos Estuary System, one of the most important Brazilian examples of environmental degradation. In the present work, one strain presented strong BPA tolerance and high BPA-degrading activity and could grow in a minimum medium containing BPA as the main carbon source. Strain MH137742 was identified as Shewanella haliotis, based on 16S rRNA gene sequencing and mass spectrometry identification by MALDI-TOF Biotyper. Shewanella haliotis was able to tolerate up to 150 mg L−1 of BPA and biotransform 75 mg L−1 in 10 h in a liquid culture medium. Based on the analysis of the produced metabolites by LC-MS, it was possible to predict the metabolic pathway used by this microorganism to degrade the BPA.


Bisphenol A Bioremediation Biodegradation Shewanella haliotis Estuarine environment 


Author Contribution

This statement is to certify that all Authors have seen and approved the manuscript being submitted. We attest to the fact that all Authors listed on the title page have contributed significantly to the work, have read the manuscript, attest to the validity and legitimacy of the data and its interpretation, and agree to its submission to the Applied Biochemistry and Biotechnology. On behalf of all Co-Authors, the corresponding Author shall bear full responsibility for the submission.

Funding Information

This research was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP 2013/50218-2) and by CAPES.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that there is no conflict of interest.

Supplementary material

12010_2019_2989_MOESM1_ESM.jpg (55 kb)
Supplement 1 Bisphenol A calibration curve (JPG 54 kb)
12010_2019_2989_MOESM2_ESM.jpg (89 kb)
Supplement 2 Determination of μmax to Shewanella haliotis on culture medium in absence of bisphenol A (JPG 88 kb)
12010_2019_2989_MOESM3_ESM.jpg (93 kb)
Supplement 3 Determination of μmax to Shewanella haliotis on culture medium containing 75 mg.L−1 of BPA. (JPG 93 kb)
12010_2019_2989_MOESM4_ESM.jpg (24 kb)
Supplement 4 Inoculum in solid culture medium. 1. Pseudomonas aeruginosa; 2. Pseudomonas otidis; 3. Klebsiella pneumoniae; 4. Enterobacter cloacae; 5. Bacillus cereus; 6. Bacillus therungiensis; 7. Bacillus altitutinis; 8. Acinetobacter tandoii; 9. Acinetobacter pittii; 10. Aeromonas hydrophila; 11. Rhodococcus ruber. (JPG 24 kb)
12010_2019_2989_MOESM5_ESM.jpg (73 kb)
Supplement 5 MALDI-TOF Biotyper bacteria identification (JPG 72 kb)
12010_2019_2989_MOESM6_ESM.jpg (87 kb)
Supplement 6 HPLC chromatograms regarding BPA degradation. (JPG 86 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Felipe Silva de Santana
    • 1
    • 2
    Email author
  • Louise Hase Gracioso
    • 1
  • Bruno Karolski
    • 1
  • Marcela dos Passos Galluzzi Baltazar
    • 3
  • Maria Anita Mendes
    • 3
  • Claudio Augusto Oller do Nascimento
    • 1
    • 3
  • Elen Aquino Perpetuo
    • 1
    • 2
    • 4
  1. 1.Environmental Research and Education Center, CEPEMA-POLI-USPUniversity of São PauloCubatãoBrazil
  2. 2.The Interunits Graduate Program in BiotechnologyUniversity of São PauloSão PauloBrazil
  3. 3.Chemical Engineering Department, POLI-USPUniversity of São PauloSão PauloBrazil
  4. 4.Department of Marine SciencesFederal University of São Paulo, Imar-UnifespSantosBrazil

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