Production, Partial Purification, and Biochemical Characterization of a Thermotolerant Alkaline Metallo-protease from Staphylococcus sciuri

  • Rasha Abu-KhudirEmail author
  • Maha M. Salem
  • Nanis Gamal Allam
  • Ehab M. M. Ali


Protease-producing Staphylococcus sciuri was isolated from poultry soil samples and culture conditions for protease production were optimized. The isolated protease showed a maximum activity of 235.1 U/ml. Enzyme purification procedure involved ammonium sulphate precipitation and Sephacryl S-200 HR gel filtration chromatography (GFC). The purification process resulted in the production of three protease fractions namely protease І (metallo-alkaline protease), II, and IІІ. The metallo-alkaline protease was purified to 25.49-fold with specific activity of 982.22 U/mg and 3.76% recovery. The partially purified metallo-protease was optimally active at pH 10.0 and 70 °C and exhibited thermal stability up to 50 °C. The protease activity was enhanced by Ca2+ and Mg2+, completely inhibited by Hg2+ and Cu2+, and significantly reduced by EDTA. The protease showed significant stability towards various surfactants, including SDS. The Km and Vmax values were 0.68 mg/ml and 166.66 nmol of azocasein/ml/h, respectively, while the activation energy (Ea) was 3.07 Kcal/mol. Hence, it is evident that the produced protease possesses unique characteristics and could be a plausible candidate for various industrial and biotechnological applications.


Protease Bacterial isolates Poultry waste Purification Characterization 



No funding sources had been involved in the conduction and/or preparation of this article.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Chemistry DepartmentBiochemistry Branch, Faculty of Science, Tanta UniversityTantaEgypt
  2. 2.Chemistry DepartmentCollege of Science, King Faisal UniversityAl-HofufSaudi Arabia
  3. 3.Botany DepartmentMicrobiology Unit, Faculty of Science, Tanta UniversityTantaEgypt
  4. 4.Department of BiochemistryFaculty of Science, King Abdulaziz UniversityJeddahSaudi Arabia

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