Inhibitory Effects of Vanadium-Binding Proteins Purified from the Sea Squirt Halocynthia roretzi on Adipogenesis in 3T3-L1 Adipocytes
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The inhibitory effects of vanadium-binding proteins (VBPs) from the blood plasma and the intestine of sea squirt on adipogenesis in 3T3-L1 adipocytes were examined. 3T3L-1 cells treated with VBP blood plasma decreased markedly the lipid content in maturing pre-adipocytes in a dose-dependent manner, whereas VBP intestine did not show significant effects on lipid accumulation. Both VBPs did not have significant effect on cell viability. In order to demonstrate the anti-adipogenic effects of VBP blood plasma, the expressions of several adipogenic transcription factors and enzymes were investigated by Reverse Transcriptase-Polymerase Chain Reaction. VBP blood plasma down-regulated the expressions of transcription factors; PPAR-γ, C/EBP-α, SREBP1, and FAS, but did not have significant effects on the expressions of lipolytic enzymes; HSL and LPL. Both the crude and purified VBPs significantly increased the mRNA levels of Wnt10b, FZ1, LRP6, and β-catenin, while decreased the expression of GSK-3β. Hence, VBP blood plasma inhibited adipogenesis by activating WNT/β-catenin pathway via the activation of Wnt10b. Based on the findings, VBP blood plasma decreased lipid accumulation which was mediated by decreasing adipogenesis, not by lipolysis. Therefore, VBP blood plasma could be used to treat obesity.
KeywordsAdipogenesis 3T3-L1 cells Sea squirt Vanadium-binding proteins
This research was part of the project entitled “Future Marine Technology Development” funded by the Ministry of Oceans and Fisheries, Republic of Korea.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 5.Bouzid, T., Hamel, F. G., & Lim, J. Y. (2016). Journal of Diabetes Research, 5, 75–85.Google Scholar
- 12.Yokoyama, C., Spiegelmsn, B. M., Wang, X., Briggs, M. R., Admon, A., Wu, J., Hua, X., Goldstein, J. L., & Brown, M. S. (1993). SREBP-1, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell, 75(1), 187–197.CrossRefGoogle Scholar
- 14.Yoshihara, M., Ueki, T., Yamaguchi, N., Kaminom, K., & Michibata, H. (2008). Biochimica et Biophysica Acta, 1780, 56–63.Google Scholar
- 15.Yoshinaga, M., Ueki, T., & Michibata, H. (2007). Metal binding ability of glutathione transferases conserved between two animal species, the vanadium-rich ascidian Ascidia sydneiensis samea and the schistosome Schistosoma japonicum. Biochimica et Biophysica Acta, 1770(9), 1413–1418.CrossRefGoogle Scholar
- 18.Srivastava, A. K. (1999). Molecular and Cell Biology, 206, 177–182.Google Scholar
- 22.Kruger, N. J. (1994). In methods in molecular biology. In J. M. Walker (Ed.), The Bradford method for protein quantitation (Vol. 32, pp. 9–15). Totowa: Humana.Google Scholar
- 28.Peters, K. G., Davis, M. G., Howard, B. W., Pokross, M., Rastogi, V., Diven, C., Greis, K. D., Eby-Wilkens, E., Maier, M., Evdokimov, A., Soper, S., & Genbauffe, F. (2003). Mechanism of insulin sensitization by BMOV (bis maltolato oxo vanadium); unliganded vanadium (VO4) as the active component. Journal of Inorganic Biochemistry, 96(2-3), 321–330.CrossRefGoogle Scholar
- 36.Kim, S. P., Nam, S. H., & Friedman, M. (2015). Food funct. Annual Review of Biochemistry, 6, 2939–2948.Google Scholar
- 37.Linhart, H. G., Ishimura-Oka, K., DeMayo, F., Kibe, T., Repka, D., Poindexter, B., & Darlington, G. J. (2001). C/EBP is required for differentiation of white, but not brown, adipose tissue. Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12532–12537.CrossRefGoogle Scholar
- 38.Fajas, L., Schoonjans, K., Gelman, L., Kim, J. B., Najib, J., Martin, G., Fruchart, J. C., Briggs, M., Spiegelman, B. M., & Auwerx, J. (1999). Regulation of peroxisome proliferator-activated receptor γ expression by adipocyte differentiation and determination factor 1/sterol regulatory element binding protein 1: implications for adipocyte differentiation and metabolism. Molecular and Cellular Biochemistry, 19(8), 5495–5503.CrossRefGoogle Scholar