Inhibitors Compounds on Sugarcane Bagasse Saccharification: Effects of Pretreatment Methods and Alternatives to Decrease Inhibition
- 123 Downloads
Considering bioethanol production, extensive research has been performed to decrease inhibitors produced during pretreatments, to diminish energy input, and to decrease costs. In this study, sugarcane bagasse was pretreated with NaOH, H2SO4, and water. The higher concentration of phenols, 3.3 g/L, was observed in biomass liquid fraction after alkaline pretreatment. Acid pretreatment was responsible to release considerable acetic acid concentration, 2.3 g/L, while water-based pretreatment was the only to release formic acid, 0.02 g/L. Furans derivatives were not detected in liquid fractions regardless of pretreatment. Furthermore, washing step removed most of the phenols from pretreated sugarcane bagasse. Saccharification of alkali-pretreated biomass plus polyethylene glycol (PEG) at 0.4% (w/v) enhanced 8 and 26% the glucose and the xylose release, respectively, while polyvinylpyrrolidone (PVP) also at 0.4% (w/v) increased the release by 10 and 31% of these sugars, respectively, even without washing and filtration steps. Moreover, these polymers cause above 50% activation of endoglucanase and xylanase activities which are crucial for biomass hydrolysis.
KeywordsSugarcane bagasse pretreatment Inhibitors Enzymes Polyethylene glycol Polyvinylpyrrolidone
The authors would like to thank the CNPq for the scholarship granted to the first author. This research was also supported by the Brazilian institutions FAPEMIG and CAPES.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 13.Sipos, B., Szilágyi, M., Sebestyén, Z., Perazzini, R., Dienes, D., Jakab, E., Crestini, C., & Réczey, K. (2011). Mechanism of the positive effect of poly(ethylene glycol) addition in enzymatic hydrolysis of steam pretreated lignocelluloses. Comptes Rendus Biologies, 334(11), 812–823.CrossRefGoogle Scholar
- 17.Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., & Crocker, D. (2008). Laboratory analytical procedure (LAP): determination of structural carbohydrates and lignin in biomass. Technical report: NREL/TP-510-42618. Golden: National Renewable Energy Laboratory.Google Scholar
- 20.Carvalheiro, F., Duarte, L. C., & Girio, F. M. (2008). Hemicellulose biorefineries: a review on biomass pretreatments. Journal of Scientific and Industrial Research, 67, 849–864.Google Scholar
- 21.Maitan-Alfenas, G. P., Visser, E. M., Alfenas, R. F., Nogueira, B. R. G., de Campos, G. G., Milagres, A. F., de Vries, R. P., & Guimaraes, V. M. (2015). The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: a comparative study. Bioresource Technology, 192, 670–676.CrossRefGoogle Scholar
- 23.Pu, Y., Treasure, T., Gonzalez, R., Venditti, R., & Jameel, H. (2011). Autohydrolysis pretreatment of mixed hardwoods to extract value prior to combustion. Bioresources, 6, 4856–4870.Google Scholar
- 33.Du, B., Sharma, L. N., Becker, C., Chen, S.-F., Mowery, R. A., van Walsum, G. P., & Chambliss, C. K. (2010). Effect of varying feedstock-pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates. Biotechnology and Bioengineering, 107(3), 430–440.CrossRefGoogle Scholar
- 41.Galbe, M., & Zacchi, G. (2007). Pretreatment of lignocellulosic materials for efficient bioethanol production. Advances in Biochemical Engineering Biotechnology., 108, 41–65.Google Scholar
- 43.González-Bautista, E., Santana-Morales, J. C., Ríos-Fránquez, F. X., Poggi-Varaldo, H. M., Ramos-Valdivia, A. C., Cristiani-Urbina, E., & Ponce-Noyola, T. (2017). Phenolic compounds inhibit cellulase and xylanase activities of Cellulomonas flavigena PR-22 during saccharification of sugarcane bagasse. Fuel, 196, 32–35.CrossRefGoogle Scholar
- 47.Cai, C., Qiu, X., Zheng, M., Lin, M., Lin, X., Lou, H., Zhan, X., Pang, Y., Huang, J., & Xie, L. (2017). Using polyvinylpyrrolidone to enhance the enzymatic hydrolysis of lignocelluloses by reducing the cellulase non-productive adsorption on lignin. Bioresource Technology, 227, 74–81.CrossRefGoogle Scholar
- 48.Quay, D. H. X., Bakar, F. D. A., Rabu, A., Said, M., Illias, R. M., Mahadi, N. M., Hassan, O., & Murad, A. M. A. (2011). Overexpression, purification and characterization of the Aspergillus niger endoglucanase, EglA, in Pichia pastoris. African Journal of Biotechnology, 10, 2101–2111.Google Scholar