Integrated Use of Maize Bran Residue for One-Step Phosphate Bio-Fertilizer Production
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The development of bio-fertilizer inoculants is important and desirable. Two phosphate-solubilizing Bacillus subtilis strains were inoculated onto maize bran residue (MBR), which was used as bio-fertilizer carrier and a primary source of nutrients in a medium used for semi-solid fermentation. Water holding capacity, swelling capacity, scanning electron microscopy, and shelf-life assays demonstrated that ground MBR had satisfactory properties for a bio-fertilizer carrier. The maximal soluble phosphorus (P) reached 642.7 ± 0.43 mg l−1 in an orthogonal test under the following optimal conditions: a pH of 7.0, a cultivation temperature of 31 °C, a medium water content of 160%, and a filling capacity of 500 g l−1. The bio-fertilizer produced by MBR improved the growth of wheat (Triticum aestivum L.) and Chinese cabbage (Brassica rapa pekinensis) with respect to plant height (by up to 18.36%) and the lengths of roots (by up to 34.03%, 27.22%, separately) in a pot experiment. This study integrated the production and storage of a bio-fertilizer to realize the one-step production of a solid bio-fertilizer using MBR.
KeywordsMaize bran residues Phosphate-solubilizing bacteria Bio-fertilizer Carrier Pot experiment
This work was supported by the financial support of the science and technology key project of agriculture in Henan province (Grant No. 182102110015) and postdoctoral research sponsorship in Henan province (Grant No.2015030).
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Conflict of Interest
The authors declare that they have no conflict of interest.
- 12.Pikovskaya, R. E. (1948). Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologia, 17, 362–370.Google Scholar