Advantage Assessment of Mixed Culture of Chlorella vulgaris and Yarrowia lipolytica for Treatment of Liquid Digestate of Yeast Industry and Cogeneration of Biofuel Feedstock

  • Lei Qin
  • Dong WeiEmail author
  • Zhongming Wang
  • Mohammad Asraful Alam


The symbiosis potential of microalgae and yeast is inherited with distinct advantages, providing an economical venue for their scale-up application. To assess the advantage of the mixed culture of microalgae Chlorella vulgaris and yeast Yarrowia lipolytica for treatment of liquid digestate of yeast industry (YILD) and cogeneration of biofuel feedstock, the cell growth characteristic, the nutrient removal efficiency, the energy storage potential of the mono, and mixed culture were investigated. The results indicated that the biomass concentration of the mixed culture (1.39–1.56 g/L of 5 times dilution group and 1.23–1.53 g/L of 10 times dilution group) was higher than those of mono cultures. The NH3-N and SO42− removal rates of the mixed culture were superior to mono cultures. Besides the higher lipid yield (0.073–0.154 g/L of 5 times dilution group and 0.112–0.183 g/L of 10 times dilution group), the higher yield of higher heating value (20.06–29.76 kJ/L of 5 times dilution group and 21.83–29.85 kJ/L of 10 times dilution group) was also obtained in the mixed culture. This study provides feasibility for remediation of YILD and cogeneration of biofuel feedstock using the mixed culture of microalgae and yeast.


Mixed culture Chlorella vulgaris Yarrowia lipolytica Yeast industrial wastewater Liquid digestate 



This work was supported by the National Natural Science Foundation of China (Grant No. 21606230), the Sciences and Technology of Guangzhou (Grant No. 201704030084), the Natural Science Foundation for research team of Guangdong Province (Grant No. 2016A030312007), the Science and Technology Program of Guangdong (Grant No. 2015A020216003, 2016A010105001), and the National Key Research and Development Program-China (2016YFB0601004). This work is partly supported by the 111 Project (B17018).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Lei Qin
    • 1
    • 2
    • 3
    • 4
  • Dong Wei
    • 1
    Email author
  • Zhongming Wang
    • 2
    • 3
    • 4
  • Mohammad Asraful Alam
    • 2
    • 3
    • 4
  1. 1.School of Food Science and EngineeringSouth China University of TechnologyGuangzhouChina
  2. 2.Guangzhou Institute of Energy Conversion, Chinese Academy of SciencesGuangzhouChina
  3. 3.CAS Key Laboratory of Renewable EnergyGuangzhouChina
  4. 4.Guangdong Provincial Key Laboratory of New and Renewable Energy Research and DevelopmentGuangzhouChina

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