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Applied Biochemistry and Biotechnology

, Volume 187, Issue 4, pp 1204–1219 | Cite as

Overexpression of StGA2ox1 Gene Increases the Tolerance to Abiotic Stress in Transgenic Potato (Solanum tuberosum L.) Plants

  • Jianbin Shi
  • Jian Wang
  • Ning Wang
  • Hong Zhou
  • Qinghua Xu
  • Gentu YanEmail author
Article
  • 264 Downloads

Abstract

It has been known that GA2ox is one kind of key enzyme gene in the gibberellin synthesis pathway, which plays important regulatory roles throughout plant whole growth and development. In this article, one of the GA2ox family genes, designated StGA2ox1, was isolated from potato (Solanum tuberosum L.). The full length of cDNA is 1005 bp, and the cDNA corresponds to a protein of 334 amino acids; this protein was classified in a group with NtGA2ox3 based on multiple sequence alignments and phylogenetic characterization. A plant expression vector pCAEZ1383-StGA2ox1 was established. qRT-PCR showed that the expression of RD28, DREB1, WRKY1, and SnRK2 genes in StGA2ox1 transgenic plant is higher than that in non-transformed control under dehydration, low temperature conditions, and abscisic acid treatments. Overexpression of StGA2ox1 cDNA in transgenic potato plants exhibited an improved salt, drought, exogenous hormone, and low temperature stress tolerance in comparison to the non-transformed plant. The enhanced stress tolerance may be associated with the subsequent accumulation of proline osmoprotectant in addition to a better control of chlorophyll, carotenoids, and water loss. These data suggest that the StGA2ox1 is involved in the regulation of plant growth and tolerance in potato by regulating the synthesis of gibberellin.

Keywords

Potato GA2ox1 Cloning Generation and transformation Abiotic stress 

Notes

Funding

This work was supported by the fund of basic research project of Qinghai province (2013-Z-720), basal research fund of central public-interest scientific institution (161016201707), National key laboratory of cotton biology (CB2017C14), and cotton industry technology system of Henan province (S2013-07-G01).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

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Authors and Affiliations

  1. 1.State Key Laboratory of Cotton BiologyInstitute of Cotton Research of CAASAnyangChina
  2. 2.Key Lab of Qinghai-Tibet Plateau Biotechnology/Key Laboratory of Germplasm Innovation and Utilization of Plateau Crop, Qinghai Academy of Agricultural and Forestry SciencesQinghai UniversityXiningChina

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