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Applied Biochemistry and Biotechnology

, Volume 187, Issue 2, pp 474–492 | Cite as

Removal of Fat Components in High TDS Leather Wastewater by Saline-Tolerant Lipase-Assisted Nanoporous-Activated Carbon

  • Maharaja Pounsamy
  • Swarnalatha Somasundaram
  • Saravanan Palanivel
  • Sekaran GanesanEmail author
Article
  • 75 Downloads

Abstract

The present investigation was carried out for the degradation of fatty components in high TDS containing wastewater (soak liquor) discharged from leather industry, and the degradation was achieved by saline-tolerant lipase-immobilized functionalized nanoporous-activated carbon (STLNPAC). The lipase was extracted from the halophilic organism, Bacillus cereus. The optimum conditions for lipase production such as time, 60 h; temperature, 50 °C; pH, 10; and substrate concentration, 2.5% (w/v) were determined through response surface methodology (RSM). The functionalization of NPAC was done by ethylenediamine/glutaraldehyde covalent interaction technique followed by the immobilization of saline-tolerant lipase onto FNPAC. The functional properties of STLNPAC were analyzed through instrumentation techniques such as TGA-DSC, FT-IR, XRD, and SEM images. The lipid content of soak liquor was removed by > 99% at HRT of 60 min using STLNPAC-packed bed reactor. The efficiency was evaluated by using UV-visible and FT-IR spectroscopic analyses. The degradation of lipids was best obeyed by pseudo first-order rate kinetics, and the rate constant was found to be 1.6 × 10−3 min−1. The biodegradability index of soak liquor was increased from 0.322 to 0.426, highly favorable for the complete removal of organic components in subsequent operations.

Keywords

Saline wastewater Soak liquor Immobilization Fats Nanoporous-activated carbon Lipase Leather industry 

Abbreviations

COD

Chemical oxygen demand

BOD5

Biochemical oxygen demand

TDS

Total dissolved solids

TS

Total solids

TSS

Total suspended solids

APHA

American Public Health Association

TOC

Total organic carbon

TKN

Total Kjeldahl nitrogen

TGA

Thermo gravimetric analysis

DSC

Differential scanning colorimetry

SEM

Scanning electron microscopy

XRD

X-ray powder diffraction (XRD)

FT-IR

Fourier-transform infrared spectroscopy

UV-Vis

Ultraviolet-visible spectrophotometry

HRT

Hydraulic retention time

MPs

Mucopolysaccharides

NPAC

Nanoporous-activated carbon

FNPAC

Functionalized nanoporous-activated carbon

STLNPAC

Saline-tolerant lipase-immobilized nanoporous-activated carbon

RO

Reverse osmosis

CETP

Common effluent treatment plant

SOAR

Sequential oxic-anoxic bioreactor

RSM

Response surface methodology

Notes

Acknowledgements

The authors acknowledge Director, CSIR-CLRI, India, for granting permission to carry out this research work. This research work was carried out as a part of Ph.D. program registered with University of Madras, Chennai, India.

Compliance with Ethical Standards

Conflict of Interest

We enclosed the revised manuscript entitled “Removal of fat components in high TDS leather wastewater by saline tolerant lipase assisted nanoporous activated carbon” which we are submitting for exclusive consideration of publication as an article in one of the most privileged journals “Applied Biochemistry and Biotechnology.” The present work lies on the degradation of fat content of soak liquor discharged from leathery industries to increase the biodegradability index by using the efficient saline-tolerant lipase-immobilized nanoporous-activated carbon (STLNPAC) and the lipase was produced from halophilic organism Bacillus cereus. It would be the part of remediation for the disposal of hazardous contaminated salt eject from tanneries by way of effective treatment of high TDS containing tannery soak liquor.

All authors are aware of and agree to the content of the paper. We certify that the submission is our original work and not under review at any other publication. Considering the scientific merit, and novelty of the work, I would be happy and thankful if you can consider this manuscript for publication in the esteemed journal. In addition, all the authors of this manuscript wish and agree to submit the work to “Applied Biochemistry and Biotechnology” declaring that the work has not been published/submitted or being submitted to another journal.

Supplementary material

12010_2018_2833_MOESM1_ESM.docx (416 kb)
ESM 1 (DOCX 416 kb)

References

  1. 1.
    Cammarota, M. C., Teixeira, G. A., & Freire, D. M. G. (2001). Enzymatic pre-hydrolysis and anaerobic degradation of wastewaters with high fat contents. Biotechnology Letters, 23(19), 1591–1595.Google Scholar
  2. 2.
    Masse, L., Kennedy, K. J., & Chou, S. (2001). Testing of alkaline and enzymatic hydrolysis pretreatments for fat particles in slaughterhouse wastewater. Bioresource Technology, 77(2), 145–155.Google Scholar
  3. 3.
    Luste, S., Luostarinen, S., & Sillanpaa, M. (2009). Effect of pre-treatments on hydrolysis and methane production potentials of by-products from meat-processing industry. Journal of Hazardous Materials, 164(1), 247–255.Google Scholar
  4. 4.
    Jayathilakan, K., Sultana, K., Radhakrishna, K., & Bawa, A. S. (2012). Utilization of byproducts and waste materials from meat, poultry and fish processing industries: A review. J Food Sci Technol, 49(3), 278–293.Google Scholar
  5. 5.
    Masse, L., Masse, D. I., & Kennedy, K. J. (2003). Effect of hydrolysis pretreatment on fat degradation during anaerobic digestion of slaughterhouse wastewater. Process Biochemistry, 38(9), 1365–1372.Google Scholar
  6. 6.
    Zupancic, G. D., & Jemec, A. (2010). Anaerobic digestion of tannery waste: Semi-continuous and anaerobic sequencing batch reactor processes. Bioresource Technology, 101(1), 26–33.Google Scholar
  7. 7.
    Li, J., Yun, Y. Q., Xing, L., & Song, L. (2017). Novel bioflocculant produced by salt-tolerant, alkaliphilic strain Oceanobacilluspolygoni HG6 and its application in tannery wastewater treatment. Bioscience, Biotechnology, and Biochemistry, 1–8.Google Scholar
  8. 8.
    Lefebvre, O., Vasudevan, N., Torrijos, M., Thanasekaran, K., & Moletta, R. (2005). Halophilic biological treatment of tannery soak liquor in a sequencing batch reactor. Water Research, 39(8), 1471–1480.Google Scholar
  9. 9.
    Rajeswari, S., Vidhya, S., Sundarapandiyan, S., Saravanan, P., Ponmariappan, S., & Vidya, K. (2016). Improvement in treatment of soak liquor by combining electro-oxidation and biodegradation. RSC Advances, 6(53), 47220–47228.Google Scholar
  10. 10.
    Boopathy, R., & Sekaran, G. (2013). Electrochemical treatment of evaporated residue of soak liquor generated from leather industry. Journal of Hazardous Materials, 260, 286–295.Google Scholar
  11. 11.
    Bautista, M. E., Pérez, L., García, M. T., Cuadros, S., & Marsal, A. (2015). Valorization of tannery wastes: Lipoamino acid surfactant mixtures from the protein fraction of process wastewater. Chemical Engineer, 262, 399–408.Google Scholar
  12. 12.
    Sekaran, G., Karthikeyan, S., Boopathy, R., Maharaja, P., Gupta, V. K., & Anandan, C. (2014). Response surface modeling for optimization heterocatalytic Fenton oxidation of persistence organic pollution in high total dissolved solid containing wastewater. Environmental Science and Pollution Research, 21(2), 1489–1502.Google Scholar
  13. 13.
    CPCB. (2009). Central Pollution Control Board (CPCB). Ministry of Environment & Forests Control of Urban Pollution Series, Cups/2009–10.Google Scholar
  14. 14.
    Petruy, R., & Lettinga, G. (1997). Digestion of a milk-fat emulsion. Bioresource Technology, 61(2), 141–149.Google Scholar
  15. 15.
    Lefebvre, O., & Moletta, R. (2006). Treatment of organic pollution in industrial saline wastewater: a literature review. Water Research, 40(20), 3671–3682.Google Scholar
  16. 16.
    Boopathy, R., Karthikeyan, S., Mandal, A. B., & Sekaran, G. (2013). Characterisation and recovery of sodium chloride from salt-laden solid waste generated from leather industry. Clean Technologies and Environmental Policy, 15(1), 117–124.Google Scholar
  17. 17.
    Mirbolooki, H., Amirnezhad, R., & Pendashteh, A. R. (2017). Treatment of high saline textile wastewater by activated sludge microorganisms. Journal of Applied Research and Technology, 15(2), 167–172.Google Scholar
  18. 18.
    Dincer, A. R., & Kargi, F. (2001). Performance of rotating biological disc system treating saline wastewater. Process Biochemistry, 36(8), 901–906.Google Scholar
  19. 19.
    Jenkins, D., Richard, M. G., & Daigger, G. T. (1993). Manual on the causes and control of activated sludge bulking and foaming (second ed.). Michigan: Lewis Publishers.Google Scholar
  20. 20.
    Vidal, G., Carvalho, A., Mendez, R., & Lema, J. M. (2000). Influence of the content in fats and proteins on the anaerobic biodegradability of dairy wastewaters. Bioresource Technology, 74(3), 231–239.Google Scholar
  21. 21.
    Boopathy, R., & Sekaran, G. (2014). Electrochemical treatment of reverse osmosis concentrate generated by the leather industry using a Cu–graphite electrode. RSC Advances, 4(20), 9971–9979.Google Scholar
  22. 22.
    Li, D., & Wang, H. (2010). Recent developments in reverse osmosis desalination membranes. Journal of Materials Chemistry, 20(22), 4551–4566.Google Scholar
  23. 23.
    Maharaja, P., Mahesh, M., Chitra, C., Kalaivani, D., Srividya, R., Swarnalatha, S., & Sekaran, G. (2017). Sequential oxic-anoxic bioreactor for the treatment of tannery saline wastewater using halophilic and filamentous bacteria. Journal of Water Process Engineering, 18, 47–57.Google Scholar
  24. 24.
    Mendes, A. A., Castro, H. F. D., Pereira, E. B., & Furigo Junior, A. (2005). Application of lipases for wastewater treatment containing high levels of lipids. Quimica Nova, 28(2), 296–305.Google Scholar
  25. 25.
    Cao, L. (2006). Carrier-bound immobilized enzymes: Principles, application and design. Wiley.Google Scholar
  26. 26.
    Sheldon, R. A., & Van Pelt, S. (2013). Enzyme immobilisation in biocatalysis: Why, what and how. Chemical Society Reviews, 42(15), 6223–6235.Google Scholar
  27. 27.
    Mahesh, M., Arivizhivendhan, K. V., Maharaja, P., Boopathy, R., Hamsavathani, V., & Sekaran, G. (2016). Production, purification and immobilization of pectinase from Aspergillus ibericus onto functionalized nanoporous activated carbon (FNAC) and its application on treatment of pectin containing wastewater. Journal of Molecular Catalysis B: Enzymatic, 133, 43–54.Google Scholar
  28. 28.
    Maharaja, P., Mahesh, M., Sunkapur, L. K., Swarnalatha, S., & Sekaran, G. (2017). Treatment of tannery saline wastewater by using effective immobilized protease catalyst produced from salt tolerant Enterococcus feacalis. Journal of Environmental Chemical Engineering, 5(2), 2042–2055.Google Scholar
  29. 29.
    APHA. (1998). AWWA, WEF, standard methods for the examination of water and wastewater, 20th edn.Google Scholar
  30. 30.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. boil. Chem., 193(1), 265–275.Google Scholar
  31. 31.
    Frings, C. S., Fendley, T. W., Dunn, R. T., & Queen, C. A. (1972). Improved determination of total serum lipids by the sulfo-phospho-vanillin reaction. Clinical Chemistry, 18(7), 673–674.Google Scholar
  32. 32.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. T., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356.Google Scholar
  33. 33.
    Jeejeebhoy, K. N., Ahmad, S., & Kozak, G. (1970). Determination of fecal fats containing both medium and long chain triglycerides and fatty acids. Clinical Biochemistry, 3(2), 157–163.Google Scholar
  34. 34.
    Lambert, M., & Neish, A. C. (1950). Rapid method for estimation of glycerol in fermentation solutions. Canadian Journal of Research, 28(3), 83–89.Google Scholar
  35. 35.
    Kanmani, P., Kumaresan, K., & Aravind, J. (2015). Utilization of coconut oil mill waste as a substrate for optimized lipase production, oil biodegradation and enzyme purification studies in Staphylococcus pasteuri. Electronic Journal of Biotechnology, 18(1), 20–28.Google Scholar
  36. 36.
    Kennedy, L. J., Vijaya, J. J., & Sekaran, G. (2004). Effect of two-stage process on the preparation and characterization of porous carbon composite from rice husk by phosphoric acid activation. Industrial and Engineering Chemistry Research, 43(8), 1832–1838.Google Scholar
  37. 37.
    Sekaran, G., Karthikeyan, S., Gupta, V. K., Boopathy, R., & Maharaja, P. (2013). Immobilization of Bacillus sp. in mesoporous activated carbon for degradation of sulphonated phenolic compound in wastewater. Materials Science and Engineering: C, 33(2), 735–745.Google Scholar
  38. 38.
    Karthikeyan, S., Sekaran, G., & Gupta, V. K. (2013). Nanoporous activated carbon fluidized bed catalytic oxidations of aqueous o, p and m-cresols: kinetic and thermodynamic studies. Environmental Science and Pollution Research International, 20(7), 4790–4806.Google Scholar
  39. 39.
    Sekaran, G., Karthikeyan, S., Nagalakshmi, C., & Mandal, A. B. (2013). Integrated Bacillus sp. immobilized cell reactor and Synechocystis sp. algal reactor for the treatment of tannery wastewater. Environmental Science and Pollution Research, 20(1), 281–291.Google Scholar
  40. 40.
    Barbosa, O., Torres, R., Ortiz, C., Berenguer-Murcia, Á., Rodrigues, R. C., & Fernandez-Lafuente, R. (2013). Heterofunctional supports in enzyme immobilization: From traditional immobilization protocols to opportunities in tuning enzyme properties. Biomacromolecules, 14(8), 2433–2462.Google Scholar
  41. 41.
    Zhang, Y., Ge, J., & Liu, Z. (2015). Enhanced activity of immobilized or chemically modified enzymes. ACS Catalysis, 5(8), 4503–4513.Google Scholar
  42. 42.
    Mateo, C., Palomo, J. M., Fernandez-Lorente, G., Guisan, J. M., & Fernandez-Lafuente, R. (2007). Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme and Microbial Technology, 40(6), 1451–1463.Google Scholar
  43. 43.
    Ozkaya, B. (2006). Adsorption and desorption of phenol on activated carbon and a comparison of isotherm models. Journal of Hazardous Materials, 129(1), 158–163.Google Scholar
  44. 44.
    Maharaja, P., Gokul, E., Prabhakaran, N., Karthikeyan, S., Boopathy, R., Swarnalatha, S., & Sekaran, G. (2016). Simultaneous removal of NH 4+-N and refractory organics through sequential heterogeneous Fenton oxidation process and struvite precipitation: Kinetic study. RSC Advances, 6(5), 4250–4261.Google Scholar
  45. 45.
    Beg, M. A. A., & Qaiser, M. A. (1992). Thermal behaviour of bis (diphenyltrifluoromethylphosphine) dichloroplatinum (II). Thermochimica Acta, 210, 123–132.Google Scholar
  46. 46.
    Manivannan, A., Chirila, M., Giles, N. C., & Seehra, M. S. (1999). Microstructure, dangling bonds and impurities in activated carbons. Carbon, 37(11), 1741–1747.Google Scholar
  47. 47.
    Silva, G. S., Oliveira, P. C., Giordani, D. S., & Castro, H. F. D. (2011). Chitosan/siloxane hybrid polymer: synthesis, characterization and performance as a support for immobilizing enzyme. Journal of the Brazilian Chemical Society, 22(8), 1407–1417.Google Scholar
  48. 48.
    Perle, M., Kimchie, S., & Shelef, G. (1995). Some biochemical aspects of the anaerobic degradation of dairy wastewater. Water Research, 29(6), 1549–1554.Google Scholar
  49. 49.
    Lin, J. G., Chang, C. N., & Chang, S. C. (1997). Enhancement of anaerobic digestion of waste activated sludge by alkaline solubilization. Bioresource Technology, 62(3), 85–90.Google Scholar
  50. 50.
    LaPara, T. M., & Alleman, J. E. (1999). Thermophilic aerobic biological wastewater treatment. Water Research, 33(4), 895–908.Google Scholar
  51. 51.
    Sung, J. H., Ahn, S. J., Kim, N. Y., Jeong, S. K., Kim, J. K., Chung, J. K., & Lee, H. H. (2010). Purification, molecular cloning, and biochemical characterization of subtilis in JB1 from a newly isolated Bacillus subtilis JB1. Applied Biochemistry and Biotechnology, 162(3), 900–911.Google Scholar
  52. 52.
    Cammarota M, Freire DG, Anna Jr GLS, Russo C, Freire DDDC and Castilho LDR (2001). Cammarota Magali Christe, Guimaraes Freire Denise Maria, Geraldo LippelSant Anna and Jr. Production process and composition of an enzymatic preparation, and its use for the treatment of domestic and industrial effluents of high fat, protein and/or carbohydrate content. U.S. Patent Application 10/399, 266.Google Scholar
  53. 53.
    Angelidaki, I., & Ahring, B. K. (1992). Effects of free long-chain fatty acids on thermophilic anaerobic digestion. Applied Microbiology and Biotechnology, 37(6), 808–812.Google Scholar
  54. 54.
    Czarnik-Matusewicz, B., Murayama, K., Wu, Y., & Ozaki, Y. (2000). Two-dimensional attenuated total reflection/infrared correlation spectroscopy of adsorption-induced and concentration-dependent spectral variations of β-lactoglobulin in aqueous solutions. The Journal of Physical Chemistry. B, 104(32), 7803–7811.Google Scholar
  55. 55.
    Mannacharaju, M., Natarajan, P., Villalan, A. K., Jothieswari, M., Somasundaram, S., & Ganesan, S. (2017). An innovative approach to minimize excess sludge production in sewage treatment using integrated bioreactors. Journal of Environmental Sciences, 67, 67–77.  https://doi.org/10.1016/j.jes.2017.07.020.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Maharaja Pounsamy
    • 1
    • 2
  • Swarnalatha Somasundaram
    • 2
  • Saravanan Palanivel
    • 1
  • Sekaran Ganesan
    • 2
    Email author
  1. 1.Leather Process Technology Lab, Central Leather Research Institute (CLRI)Council of Scientific & Industrial Research (CSIR)ChennaiIndia
  2. 2.Environmental Science and Engineering Division, Central Leather Research Institute (CLRI)Council of Scientific & Industrial Research (CSIR)ChennaiIndia

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