Applied Biochemistry and Biotechnology

, Volume 187, Issue 1, pp 310–322 | Cite as

Anti-Diabetic and Anti-Nephritic Activities of Grifola frondosa Mycelium Polysaccharides in Diet-Streptozotocin-Induced Diabetic Rats Via Modulation on Oxidative Stress

  • Ling Kou
  • Mingzhao Du
  • Peijing Liu
  • Baohai Zhang
  • Yizhi Zhang
  • Ping Yang
  • Mengyuan Shang
  • Xiaodong Wang


Grifola frondosa is an edible fungus with a variety of potential pharmacological activities. This study investigates the hypoglycemic, anti-diabetic nephritic, and antioxidant properties of G. frondosa polysaccharides in diet-streptozotocin-induced diabetic rats. After a 4-week treatment with 100 mg/kg of metformin and 200 mg/kg of one of four different G. frondosa polysaccharide mixtures (especially GFPS3 and GFPS4), diabetic rats had enhanced body weight and suppressed plasma glucose, indicating the hypoglycemic activities of the G. frondosa polysaccharides. G. frondosa polysaccharides regulated the level of serum creatinine, blood urea nitrogen, N-acetyl-β-d-glucosaminidase, and albuminuria; inhibited the serum levels of interleukin (IL)-2, IL-6, and TNF-α; and enhanced the serum levels of matrix metalloproteinase 9 and interferon-α, confirming their anti-diabetic nephritic activities. G. frondosa polysaccharides ameliorated the pathological alterations in the kidneys of diabetic rats. Moreover, G. frondosa polysaccharides modulated the serum levels of oxidant factors such as superoxide dismutase, glutathione peroxidase, catalase, malondialdehyde, and reactive oxygen species, revealing their antioxidant properties. Furthermore, the administration of G. frondosa polysaccharides inhibited nuclear factor kappa B activities in the serum and kidneys. All of the data revealed that the activation of nuclear factor kappa B plays a central role in G. frondosa polysaccharide-mediated anti-diabetic and anti-nephritic activities.


Grifola frondosa Anti-diabetes Nephritic protection Oxidative stress Nuclear factor kappa B 



This work is supported by the Project from Health and Family Planning Commission Project from Jiangsu province (no. H201536) and Scientific Research Program of the Affiliated Hospital of Jiangsu University in China (no. jdfyRC-2015004).

Compliance with Ethical Standards

Conflict of Interest

The authors have declared that there is no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ling Kou
    • 1
  • Mingzhao Du
    • 1
  • Peijing Liu
    • 1
  • Baohai Zhang
    • 1
  • Yizhi Zhang
    • 2
  • Ping Yang
    • 1
  • Mengyuan Shang
    • 1
  • Xiaodong Wang
    • 1
  1. 1.Affiliated Hospital of Jiangsu UniversityJiangsu UniversityZhenjiangChina
  2. 2.Department of Neurology, the Second Hospital of Jilin UniversityJilin UniversityChangchunChina

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