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Applied Biochemistry and Biotechnology

, Volume 187, Issue 2, pp 649–661 | Cite as

Enhancement of Tryptic Digestibility of Milk β-Lactoglobulin Through Treatment with Recombinant Rice Glutathione/Thioredoxin and NADPH Thioredoxin Reductase/Thioredoxin Systems

  • Tahere Shahriari-Farfani
  • Azar ShahpiriEmail author
  • Asghar Taheri-Kafrani
Article
  • 73 Downloads

Abstract

β-Lactoglobulin (BLG), a member of lipocalin family, is one of the major bovine milk allergens. This protein exists as a dimer of two identical subunits and contains two intramolecular disulfide bonds that are responsible for its resistance to trypsin digestion and allergenicity. This study aimed to evaluate the effect of reduction of disulfide bonds of BLG with different rice thioredoxins (Trxs) on its digestibility and allergenicity. Therefore, the active recombinant forms of three rice Trx isoforms (OsTrx1, OsTrx20, and OsTrx23) and one rice NADPH-dependent Trx reductase isoform (OsNTRB) were expressed in Escherichia coli. Based on SDS-PAGE, HPLC analysis, and competitive ELISA, the reduction of disulfide bonds of BLG with OsNTRB/OsTrx23, OsNTRB/OsTrx1, GSH/OsTrx1, or GSH/OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity. The finding of this study opens new insights for application of plant Trxs in the improvement of food protein digestibility. Especially, the use of OsTrx20 and OsTrx1 are more cost-effective than E. coli and animal Trxs due to their reduction by GSH and no need to NADPH and Trx reductase as mediator enzyme.

Keywords

Milk β-Lactoglobulin Allergenicity Digestibility Thioredoxin NADPH-dependent thioredoxin reductase Glutathione 

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Tahere Shahriari-Farfani
    • 1
  • Azar Shahpiri
    • 1
    Email author
  • Asghar Taheri-Kafrani
    • 2
  1. 1.Department of Biotechnology, College of AgricultureIsfahan University of TechnologyIsfahanIran
  2. 2.Department of Biotechnology, Faculty of advanced Sciences and TechnologiesUniversity of IsfahanIsfahanIran

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