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Applied Biochemistry and Biotechnology

, Volume 187, Issue 2, pp 628–648 | Cite as

Antimicrobial Potential of Fungal Endophytes from Moringa oleifera

  • Daljit Singh AroraEmail author
  • Navdeep Kaur
Article

Abstract

The present study was aimed to isolate the endophytic fungi having antimicrobial potential from Moringa oleifera. Out of the active isolates, the endophytic fungal isolate DSE 17 obtained from the bark of the plant was selected for further studies and identified as Aspergillus fumigatus. The classical method for optimization strategy revealed inoculum size of four discs in Czapek dox’s medium at a temperature of 25 °C and pH 7 with the incubation period of 6 days to be the best. Sucrose as carbon source (1%) and sodium nitrate as nitrogen source (0.2%) were found to be the best for antimicrobial activity. Response surface methodology was effective in optimizing the selected medium components in Plackett–Burman design, i.e. magnesium sulphate, dipotassium phosphate and sodium nitrate, which resulted in increase in antimicrobial activity by 1.7-fold. Chloroform was found to be the best extractant amongst different solvents. The minimum inhibitory concentration (MIC) values of the chloroformic extract ranged from 0.05 to 0.5 mg/ml, and the viable cell count studies revealed it to be bactericidal in its nature. The post-antibiotic effect (PAE) of the chloroformic extracts ranged from 2 to 20 h. Ames mutagenicity testing and MTT assay revealed the crude extract neither cytotoxic nor mutagenic, thus showing it to be biosafe. Thus, the study suggests that endophytes from this miracle plant could be potential source for the production of broad-spectrum antimicrobial compound/s.

Keywords

Endophytes Antimicrobial Moringa oleifera Biosafety 

Notes

Authors’ Contributions

DSA, as a principle investigator (PI), contributed substantially in the interpretation of data. NK performed the experimental work. Both the authors contributed in designing the experiments and drafting the manuscript.

Funding Information

The authors are thankful to the University Grants Commission (UGC), New Delhi, for providing financial assistance in the form of Major Research Project (MRP) sanctioned to Daljit Singh Arora.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Ethics Approval

Not applicable.

Informed Consent

This article does not contain any studies with human participants performed by any of the authors, so the consent to participate is not applicable.

Consent for Publication

Not applicable.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Microbial Technology Laboratory, Department of MicrobiologyGuru Nanak Dev UniversityAmritsarIndia

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