Detoxification of Organosolv-Pretreated Pine Prehydrolysates with Anion Resin and Cysteine for Butanol Fermentation
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Bioconversion of lignocellulose to biofuels suffers from the degradation compounds formed during pretreatment and acid hydrolysis. In order to achieve an efficient biomass to biofuel conversion, detoxification is often required before enzymatic hydrolysis and microbial fermentation. Prehydrolysates from ethanol organosolv-pretreated pine wood were used as substrates in butanol fermentation in this study. Six detoxification approaches were studied and compared, including overliming, anion exchange resin, nonionic resin, laccase, activated carbon, and cysteine. It was observed that detoxification by anion exchange resin was the most effective method. The final butanol yield after anion exchange resin treatment was comparable to the control group, but the fermentation was delayed for 72 h. The addition of Ca(OH)2 was found to alleviate this delay and improve the fermentation efficiency. The combination of Ca(OH)2 and anion exchange resin resulted in completion of fermentation within 72 h and acetone–butanol–ethanol (ABE) production of 11.11 g/L, corresponding to a yield of 0.21 g/g sugar. The cysteine detoxification also resulted in good detoxification performance, but promoted fermentation towards acid production (8.90 g/L). The effect of salt on ABE fermentation was assessed and the possible role of Ca(OH)2 was to remove the salts in the prehydrolysates by precipitation.
KeywordsDetoxification Fermentation Butanol Organosolv pretreatment
This study received financial support from the National Science Foundation (NSF-CBET 1555633), Southeastern Sun Grant Center, United States Department of Agriculture (USDA-2010-38502-21854), and the United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) through the Integrated Biomass Supply Systems (IBSS) project (2011-68005-30410).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 1.Sheelanere, P., & Kulshreshtha, S. (2013). Sustainable biofuel production: opportunities for rural development. International Journal of Environment and Resource, 2, 1–13.Google Scholar
- 5.Klinke, H. B., Olsson, L., Thomsen, A. B., & Ahring, B. K. (2003). Potential inhibitors from wet oxidation of wheat straw and their effect on ethanol production of Saccharomyces cerevisiae: wet oxidation and fermentation by yeast. Biotechnology and Bioengineering, 81(6), 738–747.CrossRefGoogle Scholar
- 14.Persson, P., Andersson, J., Gorton, L., Larsson, S., Nilvebrant, N.-O., & Jönsson, L. J. (2002). Effect of different forms of alkali treatment on specific fermentation inhibitors and on the fermentability of lignocellulose hydrolysates for production of fuel ethanol. Journal of Agricultural and Food Chemistry, 50(19), 5318–5325.CrossRefGoogle Scholar
- 16.Ranatunga, T. D., Jervis, J., Helm, R. F., McMillan, J. D., & Wooley, R. J. (2000). The effect of overliming on the toxicity of dilute acid pretreated lignocellulosics: the role of inorganics, uronic acids and ether-soluble organics. Enzyme and Microbial Technology, 27(3-5), 240–247.CrossRefGoogle Scholar
- 18.Liu, K., Atiyeh, H. K., Pardo-Planas, O., Ezeji, T. C., Ujor, V., Overton, J. C., Berning, K., Wilkins, M. R., & Tanner, R. S. (2015). Butanol production from hydrothermolysis-pretreated switchgrass: quantification of inhibitors and detoxification of hydrolyzate. Bioresource Technology, 189, 292–301.CrossRefGoogle Scholar
- 23.Pan, X., Arato, C., Gilkes, N., Gregg, D., Mabee, W., Pye, K., Xiao, Z., Zhang, X., & Saddler, J. (2005). Biorefining of softwoods using ethanol organosolv pulping: preliminary evaluation of process streams for manufacture of fuel-grade ethanol and co-products. Biotechnology and Bioengineering, 90(4), 473–481.CrossRefGoogle Scholar
- 25.Pan, X., Xie, D., Kang, K.-Y., Yoon, S.-L., & Saddler, J. N. (2007). Effect of organosolv ethanol pretreatment variables on physical characteristics of hybrid poplar substrates. Appl Biochem Biotecnol, 136-140, 367–378.Google Scholar
- 26.Pan, X., Gilkes, N., Kadla, J., Pye, K., Saka, S., Gregg, D., Ehara, K., Xie, D., Lam, D., & Saddler, J. (2006). Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: optimization of process yields. Biotechnology and Bioengineering, 94(5), 851–861.CrossRefGoogle Scholar
- 34.Qureshi, N., Saha, B. C., Hector, R. E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M. J., Sarath, G., & Cotta, M. A. (2010). Production of butanol (a biofuel) from agricultural residues: part II—use of corn Stover and switchgrass hydrolysates. Biomass and Bioenergy, 34(4), 566–571.CrossRefGoogle Scholar
- 40.Luo, H., Ge, L., Zhang, J., Zhao, Y., Ding, J., Li, Z., He, Z., Chen, R., & Shi, Z. (2015). Enhancing butanol production under the stress environments of co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae integrated with exogenous butyrate addition. PLoS One, 10(10), e0141160.CrossRefGoogle Scholar
- 41.Heluane, H., Evans, M. R., Dagher, S. F., & Bruno-Bárcena, J. M. (2011). Meta-analysis and functional validation of nutritional requirements of solventogenic Clostridia growing under butanol stress conditions and coutilization of D-glucose and D-xylose. Applied and Environmental Microbiology, 77(13), 4473–4485.CrossRefGoogle Scholar
- 43.Maddox, I., Qureshi, N., & Roberts-Thomson, K. (1995). Production of acetone-butanol-ethanol from concentrated substrate using clostridium acetobutylicum in an integrated fermentation-product removal process. Process Biochem, 30, 209–215.Google Scholar
- 44.Qureshi, N., Saha, B. C., Hector, R. E., & Cotta, M. A. (2008). Removal of fermentation inhibitors from alkaline peroxide pretreated and enzymatically hydrolyzed wheat straw: production of butanol from hydrolysate using Clostridium beijerinckii in batch reactors. Biomass and Bioenergy, 32(12), 1353–1358.CrossRefGoogle Scholar