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Applied Biochemistry and Biotechnology

, Volume 186, Issue 3, pp 779–788 | Cite as

Valorization of Bone Waste of Saudi Arabia by Synthesizing Hydroxyapatite

  • Touseef Amna
Article
  • 63 Downloads

Abstract

At present, hydroxyapatite is being frequently used for diverse biomedical applications as it possesses excellent biocompatibility, osteoconductivity, and non-immunogenic characteristics. The aim of the present work was to recycle bone waste for synthesis of hydroxyapatite nanoparticles to be used as bone extracellular matrix. For this reason, we for the first time utilized bio-waste of cow bones of Albaha city. The residual bones were utilized for the extraction of natural bone precursor hydroxyapatite. A facile scientific technique has been used to synthesize hydroxyapatite nanoparticles through calcinations of wasted cow bones without further supplementation of chemicals/compounds. The obtained hydroxyapatite powder was ascertained using physicochemical techniques such as XRD, SEM, FTIR, and EDX. These analyses clearly show that hydroxyapatite from native cow bone wastes is biologically and physicochemically comparable to standard hydroxyapatite, commonly used for biomedical functions. The cell viability and proliferation over the prepared hydroxyapatite was confirmed with CCk-8 colorimetric assay. The morphology of the cells growing over the nano-hydroxyapatite shows that natural hydroxyapatite promotes cellular attachment and proliferation. Hence, the as-prepared nano-hydroxyapatite can be considered as cost-effective source of bone precursor hydroxyapatite for bone tissue engineering. Taking into account the projected demand for reliable bone implants, the present research work suggested using environment friendly methods to convert waste of Albaha city into nano-hydroxyapatite scaffolds. Therefore, besides being an initial step towards accomplishment of projected demands of bone implants in Saudi Arabia, our study will also help in reducing the environmental burden by recycling of bone wastes of Albaha city.

Keywords

Implants Hydroxyapatite Native cow Recycling Bones 

Notes

Funding Information

This research (Proposal No. 54-1436) was supported by the Deanship for Scientific Research, University of Albaha, Albaha, Kingdom of Saudi Arabia (KSA), funded by the Ministry of Higher Education. Prof. Dr. Touseef Amna sincerely acknowledges the research grant.

Compliance with Ethical Standards

Conflict of Interest

The author declares that he has no conflict of interest.

Ethical Statement

In the present study, there is no use of experimental animals. All the experiments were done under in vitro conditions following standard scientific procedures and ethics.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biology, Faculty of ScienceAlbaha UniversityAlbahaKingdom of Saudi Arabia

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